scholarly journals Transformation of Candida albicans with a synthetic hygromycin B resistance gene

Yeast ◽  
2010 ◽  
Vol 27 (12) ◽  
pp. 1039-1048 ◽  
Author(s):  
Luiz R. Basso ◽  
Ann Bartiss ◽  
Yuxin Mao ◽  
Charles E. Gast ◽  
Paulo S. R. Coelho ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Resistance Gene ◽  
Hygromycin B ◽  
Hygromycin B Resistance

Transcriptome Responses of Hygromycin B Resistance Gene-Transformed, Hygromycin B-Adaptive and Wild Nannochloropsis oceanica Strains to Hygromycin B

2020 ◽  
Vol 19 (2) ◽  
pp. 453-458
Author(s):  
Hang Liu ◽  
Zhongyi Zhang ◽  
Sijie Liang ◽  
Li Guo ◽  
Shiyang Sun ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Hygromycin B ◽  
Nannochloropsis Oceanica ◽  
Hygromycin B Resistance

The 3′-terminal region of the hygromycin-B-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum

Gene ◽  
1991 ◽  
Vol 100 ◽  
pp. 247-250 ◽  
Author(s):  
Roland Bilang ◽  
Shigeru Iida ◽  
Alex Peterhans ◽  
Ingo Potrykus ◽  
Jerzy Paszkowski
Keyword(s):  
Escherichia Coli ◽  
Nicotiana Tabacum ◽  
Resistance Gene ◽  
Terminal Region ◽  
Hygromycin B ◽  
Hygromycin B Resistance

Transformation of Botrytis cinerea with the hygromycin B resistance gene, hph

1994 ◽  
Vol 26 (3) ◽  
pp. 251-255 ◽  
Author(s):  
W. Hamada ◽  
Ph. Reignault ◽  
G. Bompeix ◽  
M. Boccara
Keyword(s):  
Botrytis Cinerea ◽  
Resistance Gene ◽  
Hygromycin B ◽  
Hygromycin B Resistance

scholarly journals Use of a bacterial hygromycin B resistance gene as a dominant selectable marker in Neurospora crassa transformation

1989 ◽  
Vol 36 (1) ◽  
pp. 79 ◽  
Author(s):  
C. Staben ◽  
B. Jensen ◽  
M. Singer ◽  
J. Pollock ◽  
M. Schechtman ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Resistance Gene ◽  
Selectable Marker ◽  
Hygromycin B ◽  
Hygromycin B Resistance ◽  
Dominant Selectable Marker

Novel and convenient methods for Candida tropicalis gene disruption using a mutated hygromycin B resistance gene

2001 ◽  
Vol 176 (5) ◽  
pp. 364-369 ◽  
Author(s):  
Akihiro Hara ◽  
Mami Arie ◽  
Tamotsu Kanai ◽  
Toru Matsui ◽  
Hitoshi Matsuda ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Candida Tropicalis ◽  
Gene Disruption ◽  
Hygromycin B ◽  
Hygromycin B Resistance

The Transformation in Trichoderma viride Protoplasts by Restriction Enzyme Mediated Integration (REMI)

2012 ◽  
Vol 518-523 ◽  
pp. 545-548
Author(s):  
Yu Ru You ◽  
Shi Wang Liu ◽  
Yuan Feng Wu ◽  
Jun Huang ◽  
Gong Nian Xiao ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Restriction Enzyme ◽  
Phosphate Buffer ◽  
Trichoderma Viride ◽  
Hygromycin B ◽  
Hygromycin B Resistance ◽  
Protoplast Yield ◽  
Preliminary Identification ◽  
Exogenous Gene

The transformation conditions of the protoplasts from Trichoderma viride mediated by restriction enzyme were studied in this paper. The optimum generation conditions of protoplasts were as followed: 8 mg/ml glucanex was added into the phosphate buffer (pH 6.98), the mycelial that cultured for 24 hr was hydrolyzed for 4 hr at 30°C under 40 r/min shaking speed. The protoplast yield was 4.7×107 cfu/mg. The regeneneration rate of protoplast was 14.5% on CM medium contained 0.3 mol/L KCl and 0.3 mol/L Inositol. Transformants were obtained by transfering hygromycin B resistance gene into T. viride by restriction enzyme mediated integration (REMI), The preliminary identification of the transformants indicated that the exogenous gene had been integrated into T. viride genome.

scholarly journals Effect of Introns and AT-Rich Sequences on Expression of the Bacterial Hygromycin B Resistance Gene in the BasidiomyceteSchizophyllum commune

2001 ◽  
Vol 67 (1) ◽  
pp. 481-483 ◽  
Author(s):  
Karin Scholtmeijer ◽  
Han A. B. Wösten ◽  
Jan Springer ◽  
Joseph G. H. Wessels
Keyword(s):  
Resistance Gene ◽  
Schizophyllum Commune ◽  
Hygromycin B ◽  
Coding Region ◽  
Mrna Accumulation ◽  
Hygromycin B Resistance ◽  
Independent Events ◽  
Affect Expression

ABSTRACT Previously, it was shown that introns are required for efficient mRNA accumulation in Schizophyllum commune and that the presence of AT-rich sequences in the coding region of genes can result in truncation of transcripts in this homobasidiomycete. Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.

scholarly journals Transformation of Penicillium urticae with Plasmids Containing the Hygromycin B Resistance Gene.

1991 ◽  
Vol 55 (12) ◽  
pp. 3053-3057 ◽  
Author(s):  
Naomi KIUCHI ◽  
Atsushi NARUSE ◽  
Hiroki YAMAMOTO ◽  
Junichi SEKIGUCHI
Keyword(s):  
Resistance Gene ◽  
Hygromycin B ◽  
Hygromycin B Resistance

scholarly journals Transformation ofPenicillium urticaewith Plasmids Containing the Hygromycin B Resistance Gene

1991 ◽  
Vol 55 (12) ◽  
pp. 3053-3057
Author(s):  
Naomi Kiuchi ◽  
Atsushi Naruse ◽  
Hiroki Yamamoto ◽  
Junichi Sekiguchi
Keyword(s):  
Resistance Gene ◽  
Hygromycin B ◽  
Hygromycin B Resistance
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