nannochloropsis oceanica
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2021 ◽  
Vol 20 (2) ◽  
pp. 169-177
Author(s):  
Haydeé López ◽  
María Cristina Miglio ◽  
Christian Paredes

Se realizaron pruebas de toxicidad con elutriados de sedimentos marinos provenientes de las bahías Los Chimus, Tortugas y Casma, en Perú, empleando las microalgas marinas Isochrysis galbana y Nannochloropsis oceanica. Las muestras de sedimento marino se colectaron en los meses de marzo y agosto de 2016. Estas pruebas de toxicidad se realizaron siguiendo los protocolos estandarizados, mediante pruebas estáticas a 96 h de exposición. Previamente, se determinó la sensibilidad de ambas cepas de microalgas al sulfato de cobre (CuSO4·5H2O) como sustancia de referencia. La Concentración de Inhibición media (CI50) obtenida en este ensayo demostró que I. galbana fue más sensible que N. oceanica. Los elutriados de los sedimentos marinos, provenientes de las zonas de estudio, presentaron diferentes niveles de toxicidad, tales como “no toxicidad” (CI50 = 90% - 100%), “moderada toxicidad” (CI50 = 59% - 81%) y “alta toxicidad” (CI50 < 59%) al emplear la microalga I. galbana; mientras que, para el caso de N. oceanica solo se presentó el de “no toxicidad” (CI50 = 90% - 100%) frente a las mismas muestras de elutriados de sedimentos. Estas pruebas de toxicidad demuestran que la microalga I. galbana detecta diferentes niveles de toxicidad, por lo que se podría emplear para evaluar ambientalmente muestras de sedimento marino como complemento a pruebas químicas, ya que no se discrimina el origen de la toxicidad.


Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 72
Author(s):  
Maria G. Savvidou ◽  
Angelo Ferraro ◽  
Petros Schinas ◽  
Diomi Mamma ◽  
Dimitris Kekos ◽  
...  

Microalgae are used in industrial and pharmaceutical applications. Their performance on biological applications may be improved by their immobilization. This study presents a way of cell immobilization using microalgae carrying magnetic properties. Nannochloropsis oceanica and Scenedasmus almeriensis cells were treated enzymatically (cellulase) and mechanically (glass beads), generating protoplasts as a means of incorporation of magnetic nanoparticles. Scanning electron microscopy images verified the successful cell wall destruction for both of the examined microalgae cells. Subsequently, protoplasts were transformed with magnetic nanoparticles by a continuous electroporation method and then cultured on a magnetic surface. Regeneration of transformed protoplasts was optimized using various organic carbon and amino acid supplements. Both protoplast preparation methods demonstrated similar efficiency. Casamino acids, as source of amino acids, were the most efficient compound for N. oceanica protoplasts regeneration in enzymatic and mechanical treatment, while for S. almeriensis protoplasts regeneration, fructose, as source of organic carbon, was the most effective. Protoplasts transformation efficiency values with magnetic nanoparticles after enzymatic or mechanical treatments for N. oceanica and S. almeriensis were 17.8% and 10.7%, and 18.6% and 15.7%, respectively. Finally, selected magnetic cells were immobilized and grown on a vertical magnetic surface exposed to light and without any supplement.


Author(s):  
Mihris Ibnu Saleem Naduthodi ◽  
Christian Südfeld ◽  
Emmanouil Klimis Avitzigiannis ◽  
Nicola Trevisan ◽  
Eduard van Lith ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana C. M. Vítor ◽  
Alexandra E. Francisco ◽  
Joana Silva ◽  
Mário Pinho ◽  
Sharon A. Huws ◽  
...  

AbstractEicosapentaenoic acid (EPA) from freeze-dried biomass of Nannochloropsis oceanica microalgae resists ruminal biohydrogenation in vitro, but in vivo demonstration is needed. Therefore, the present study was designed to test the rumen protective effects of N. oceanica in lambs. Twenty-eight lambs were assigned to one of four diets: Control (C); and C diets supplemented with: 1.2% Nannochloropsis sp. oil (O); 12.3% spray-dried N. oceanica (SD); or 9.2% N. oceanica (FD), to achieve 3 g EPA /kg dry matter. Lambs were slaughtered after 3 weeks and digestive contents and ruminal wall samples were collected. EPA concentration in the rumen of lambs fed FD was about 50% higher than lambs fed SD or O diets. Nevertheless, the high levels of EPA in cecum and faeces of animals fed N. oceanica biomass, independently of the drying method, suggests that EPA was not completely released and absorbed in the small intestine. Furthermore, supplementation with EPA sources also affected the ruminal biohydrogenation of C18 fatty acids, mitigating the shift from the t10 biohydrogenation pathways to the t11 pathways compared to the Control diet. Overall, our results demonstrate that FD N. oceanica biomass is a natural rumen-protected source of EPA to ruminants.


2021 ◽  
Author(s):  
Christian Südfeld ◽  
Ana Pozo-Rodríguez ◽  
Sara A. Manjavacas Díez ◽  
René H. Wijffels ◽  
Maria J. Barbosa ◽  
...  

2021 ◽  
Vol 59 ◽  
pp. 102427
Author(s):  
Bárbara O. Guimarães ◽  
Kieke de Boer ◽  
Pieter Gremmen ◽  
Anemoon Drinkwaard ◽  
Rick Wieggers ◽  
...  

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