Characterization of the Cross-Linking Site of Disintegrins Albolabrin, Bitistatin, Echistatin, and Eristostatin on Isolated Human Platelet Integrin GpIIb/IIIa

Addition of wheat germ agglutinin (WGA) (50 ug/ml) to washed human platelets (3 x 108/ml) resulted in platelet activation and the release of l4C-5HT within the same time scale as 0.05 units/ml thrombin. In contrast, succinyl-WGA (100 ug/ml) induced no platelet response. The increased valency of WGA (4) compared with succinyl-WGA (2) suggests that the activation is induced through the cross-linking (immobilisation ?) of closely associated receptors in the membrane. This finding induced us to attempt to cross-link and thereby identify adjacent molecules in the membrane by “near-neighbour” analysis. Constituent -SH groups were oxidised employing Cu2+/phenanthroline or diamide as catalysts, and polymers formed as a result of intermolecular -S-S- formation between adjacent molecules were identified by SDS-PAGE. Although previous reports have shown that the major human platelet membrane glycoproteins contain -SH groups, no apparent cross-linking of the glycoproteins was located following the incubation of either washed platelets or isolated membranes with Cu2+/phenanthroline or diamide. However bidimensional SDS-PAGE (1st dimension non-reduced, 2nd dimension reduced) showed the presence of several protein polymers including complexes formed by the cross-linking of 3 large polypeptides of M. Wt. 250 000, 220 000 and 200 000. These components were easily eluted from membrane vesicles at pH 10 and may represent closely associated constituents at the cytoplasmic surface of the plasma membrane.

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Synthesis and Characterization of Large-Core Star Polymers and Polymer Networks: Effects of Arm Length and Composition of the Cross-Linking Mixture

Macromolecular Chemistry and Physics ◽

10.1002/macp.200800464 ◽

2009 ◽

Vol 210 (5) ◽

pp. 367-376 ◽

Cited By ~ 6

Author(s):

Demetris Kafouris ◽

Michael Gradzielski ◽

Costas S. Patrickios

Keyword(s):

Polymer Networks ◽

Star Polymers ◽

Synthesis And Characterization ◽

Cross Linking ◽

Large Core ◽

The Cross

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Characterization of the Cross-Linking Nylon-6,6 Blended Reactive Types of Flame Retardants with .GAMMA.-Ray Irradiation by Solid State NMR

KOBUNSHI RONBUNSHU ◽

10.1295/koron.63.799 ◽

2006 ◽

Vol 63 (12) ◽

pp. 799-804 ◽

Cited By ~ 1

Author(s):

Yuko MIWA ◽

Hiroyuki ISHIDA ◽

Toshiyuki KANNO ◽

Hiromasa YANASE ◽

Kiyotaka SHIGEHARA

Keyword(s):

Solid State ◽

Solid State Nmr ◽

Flame Retardants ◽

Gamma Ray ◽

Cross Linking ◽

Nylon 6,6 ◽

Gamma Ray Irradiation ◽

The Cross

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Investigation of a novel approach for the cross-linking characterization of SU-8 photoresist materials by means of optical dispersion measurements

10.1117/12.2039052 ◽

2014 ◽

Author(s):

Ch. Taudt ◽

T. Baselt ◽

E. Koch ◽

P. Hartmann

Keyword(s):

Cross Linking ◽

Optical Dispersion ◽

Novel Approach ◽

The Cross

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Characterization of Thermal Reversible Cross-Linking Agents for Flexible Poly(vinyl chloride)

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.900.3 ◽

2014 ◽

Vol 900 ◽

pp. 3-6 ◽

Cited By ~ 1

Author(s):

Jing Wang ◽

Wei Fang ◽

Yuan Yuan ◽

Xiao Ming Liu

Keyword(s):

Thermal Stability ◽

Vinyl Chloride ◽

Congo Red ◽

Cross Linking ◽

Gel Content ◽

Ethyl Mercaptan ◽

Poly Vinyl Chloride ◽

The Cross ◽

Thermal Stability Of

In order to improve thermal stability of Poly (Vinyl Chloride), a kind of thermal reversible cross-linking agents, the end cyclopentadienyl ethanethiol salts, sodium cyclopentadienyl ethyl mercaptan (CPD-C2H4SNa) was synthesized in this paper. The DSC results proved that the prepared CPD-C2H4SNa has obvious thermal reversible characteristics and the thermal reversible temperature was at 177 °C. The effects of cross-linking agent amount, cross-linking reactive time and temperature on flexible PVC compounds were evaluated through gel content testing. It was showed that the cross-linking agent with 3 Phr is optimum for PVC compounds. Moreover, both the cross-linking reactive time and temperature has effects on gel content of PVC compounds. The gel content of CPD-C2H4SNa cross-linked PVC compounds reaches maximum at 76% for 20 min at 160 °C.The static thermal stability of flexible PVC was measured according to congo red method. The results showed that the thermal stability of PVC compounds was enhanced with increasing cross-linking agent amount.

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