Identification of a Novel Isoform of Estrogen Receptor, a Potential Inhibitor of Estrogen Action, in Vascular Smooth Muscle Cells

1996 ◽  
Vol 219 (3) ◽  
pp. 766-772 ◽  
Author(s):  
Satoshi Inoue ◽  
Shin-jiro Hoshino ◽  
Hideyuki Miyoshi ◽  
Masahiro Akishita ◽  
Takayuki Hosoi ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Estrogen Action ◽  
Potential Inhibitor

Estradiol-mediated ERK phosphorylation and apoptosis in vascular smooth muscle cells requires GPR 30

2009 ◽  
Vol 297 (5) ◽  
pp. C1178-C1187 ◽  
Author(s):  
Qingming Ding ◽  
Robert Gros ◽  
Lee E. Limbird ◽  
Jozef Chorazyczewski ◽  
Ross D. Feldman
Keyword(s):  
Estrogen Receptor ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Pi3 Kinase ◽  
Receptor Expression ◽  
Erk Phosphorylation ◽  
Stimulation Of

Recent studies suggest that the rapid and nongenomic effects of estradiol may be mediated through the G protein-coupled receptor dubbed GPR30 receptor. The present study examines the role of GPR30 versus a classical estrogen receptor (ERα) in mediating the growth regulatory effects of estradiol. GPR30 is readily detectable in freshly isolated vascular tissue but barely detectable in cultured vascular smooth muscle cells (VSMC). In freshly isolated aortic tissue, estradiol stimulated extracellular signal-regulated kinases (ERK) phosphorylation. In contrast, in cultured VSMC, where GPR30 expression is significantly reduced, estradiol inhibits ERK phosphorylation. Transfer of the genes encoding GPR30 led to estradiol stimulation of ERK phosphorylation, which is opposite the effects of estradiol in the primary culture of VSMCs. Transduction of the mineralocorticoid receptor (MR) had no effect on estradiol effects on ERK. Estradiol-mediated stimulation of ERK subsequent to heterologous GPR30 expression was pertussis toxin sensitive and phosphoinositide 3-kinase (PI3 kinase) dependent; under these conditions, estradiol also inhibited protein kinase A (PKA). In contrast, in the absence of GPR30 expression in cultured VSMC, estradiol stimulated PKA activity and inhibited ERK phosphorylation. To determine the functional effect of GPR30 (vs. estrogen receptor expression), we assessed estradiol-mediated apoptosis. In the absence of GPR30 expression, estradiol inhibited apoptosis. This effect was enhanced with ERα expression. In contrast, with GPR30 expression, estradiol stimulated apoptosis in an ERK-dependent manner. Thus the effect of estradiol on vascular smooth muscle cell apoptosis is likely dependent on the balance between ER-mediated PKA activation and GPR30-mediated PKA inhibition and PI3 kinase activation. Taken together, we postulate that modulation of GPR30 expression or activity may be an important determinant of the effects of estradiol in the vasculature.

scholarly journals Estrogen Receptor–Mediated Regulation of MicroRNA Inhibits Proliferation of Vascular Smooth Muscle Cells

2013 ◽  
Vol 33 (2) ◽  
pp. 257-265 ◽  
Author(s):  
Jin Zhao ◽  
Gregory A. Imbrie ◽  
Wendy E. Baur ◽  
Lakshmanan K. Iyer ◽  
Mark J. Aronovitz ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells

Vascular Smooth Muscle Cells Possess Estrogen Receptor and Respond to Estrogena

2006 ◽  
Vol 748 (1) ◽  
pp. 592-594 ◽  
Author(s):  
AKIRA ORIMO ◽  
SATOSHI INOUE ◽  
YASUYOSHI OUCHI ◽  
HAJIME ORIMO
Keyword(s):  
Estrogen Receptor ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells

Methoxyestradiols Mediate the Antimitogenic Effects of Estradiol on Vascular Smooth Muscle Cells via Estrogen Receptor-Independent Mechanisms

2000 ◽  
Vol 278 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Raghvendra K. Dubey ◽  
Delbert G. Gillespie ◽  
Lefteris C. Zacharia ◽  
Marinella Rosselli ◽  
Kenneth R. Korzekwa ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells

scholarly journals Human vascular smooth muscle cells express an estrogen receptor isoform

FEBS Letters ◽  
1995 ◽  
Vol 377 (2) ◽  
pp. 103-108 ◽  
Author(s):  
Richard H. Karas ◽  
Wendy E. Baur ◽  
Martin van Eickles ◽  
Michael E. Mendelsohn
Keyword(s):  
Estrogen Receptor ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells
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