Lysophosphatidic Acid Stimulates p21-Activated Kinase in Vascular Smooth Muscle Cells

2002 ◽  
Vol 291 (3) ◽  
pp. 687-691 ◽  
Author(s):  
Udo Schmitz ◽  
Kerstin Thömmes ◽  
Imke Beier ◽  
Hans Vetter
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Lysophosphatidic Acid ◽  
Muscle Cells ◽  
P21 Activated Kinase

Angiotensin II-induced activation of p21-activated kinase 1 requires Ca2+ and protein kinase Cδ in vascular smooth muscle cells

2005 ◽  
Vol 289 (5) ◽  
pp. C1286-C1294 ◽  
Author(s):  
Elethia A. Woolfolk ◽  
Satoru Eguchi ◽  
Haruhiko Ohtsu ◽  
Hidekatsu Nakashima ◽  
Hikaru Ueno ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Growth Factor Receptor ◽  
Muscle Cells ◽  
Dominant Negative ◽  
Ang Ii ◽  
Signaling Mechanism ◽  
P21 Activated Kinase

ANG II promotes remodeling of vascular smooth muscle cells (VSMCs) in cardiovascular diseases. It has been shown to activate p21-activated kinase (PAK)1, a critical component of signaling pathways implicated in growth and migration. However, the detailed signaling mechanism by which ANG II induces PAK1 activation in VSMCs remains unclear. Therefore, we have examined the mechanism required for activation of PAK1 by ANG II in VSMCs. ANG II, through activation of the ANG II type 1 receptor, rapidly promotes phosphorylation of PAK1 in VSMCs via a pathway independent of transactivation of the epidermal growth factor receptor. Using selective agonists and inhibitors, we demonstrated that mobilization of intracellular Ca2+ and PKCδ activation are required for ANG II-induced PAK1 phosphorylation. Rottlerin, a PKCδ inhibitor, significantly blocked ANG II-induced PAK1 phosphorylation. Further support for this notion was provided through infection of VSMCs with adenovirus encoding a dominant-negative (dn)PKCδ, which also markedly reduced phosphorylation of PAK1 by ANG II. In this pathway, Ca2+ acts upstream of PKCδ because a Ca2+ ionophore rapidly induced PKCδ phosphorylation at Tyr311 and Ca2+-dependent PAK1 phosphorylation was blocked by rottlerin. In addition, dnPYK-2, dnRac, and antioxidants inhibited ANG II-induced PAK1 phosphorylation, suggesting that PYK-2, Rac, and reactive oxygen species are involved in the upstream signaling. Finally, dnPAK1 markedly inhibited ANG II-induced protein synthesis in VSMCs. These data provide a novel signaling pathway by which ANG II may contribute to vascular remodeling.

scholarly journals Lysophosphatidic Acid‐Induced Integrin Activation in Vascular Smooth Muscle Cells Requires Production of Reactive Oxygen Species

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Marius Catalin Staiculescu ◽  
Zhongkui Hong ◽  
Zhe Sun ◽  
Francisco Ramirez‐Perez ◽  
Gerald A. Meininger ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Lysophosphatidic Acid ◽  
Reactive Oxygen ◽  
Muscle Cells ◽  
Oxygen Species ◽  
Integrin Activation

Effects of Lysophosphatidic Acid on Proliferation and Cytosolic Ca++ of Human Adult Vascular Smooth Muscle Cells in Culture

1999 ◽  
Vol 94 (5) ◽  
pp. 317-326 ◽  
Author(s):  
Isabelle Gennero ◽  
Jean-Marie Xuereb ◽  
Marie-Françoise Simon ◽  
Jean-Pierre Girolami ◽  
Jean-Louis Bascands ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Lysophosphatidic Acid ◽  
Muscle Cells ◽  
Cells In Culture ◽  
Human Adult

scholarly journals Angiotensin II Stimulates p21-Activated Kinase in Vascular Smooth Muscle Cells

1998 ◽  
Vol 82 (12) ◽  
pp. 1272-1278 ◽  
Author(s):  
Udo Schmitz ◽  
Takafumi Ishida ◽  
Mari Ishida ◽  
James Surapisitchat ◽  
Mohammed I. Hasham ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
P21 Activated Kinase

Mechanisms of Lysophosphatidic Acid–induced DNA Synthesis in Vascular Smooth Muscle Cells

2003 ◽  
Vol 41 (3) ◽  
pp. 381-387 ◽  
Author(s):  
Yan-Jun Xu ◽  
Satyajeet S. Rathi ◽  
Donald C. Chapman ◽  
Amarjit S. Arneja ◽  
Naranjan S. Dhalla
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Vascular Smooth Muscle Cells ◽  
Lysophosphatidic Acid ◽  
Muscle Cells

Mechanisms of lysophosphatidic acid-induced increase in intracellular calcium in vascular smooth muscle cells

Cell Calcium ◽  
2005 ◽  
Vol 38 (6) ◽  
pp. 569-579 ◽  
Author(s):  
Yan-Jun Xu ◽  
Harjot K. Saini ◽  
Sukhinder K. Cheema ◽  
Naranjan S. Dhalla
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Intracellular Calcium ◽  
Vascular Smooth Muscle Cells ◽  
Lysophosphatidic Acid ◽  
Muscle Cells
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