Immunohistochemical Analysis of the Brachyury Protein in Wild-Type and Mutant Mouse Embryos

1994 ◽  
Vol 161 (1) ◽  
pp. 179-193 ◽  
Author(s):  
Andreas Kispert ◽  
Bernhard G. Herrmann
Keyword(s):  
Mutant Mouse ◽  
Immunohistochemical Analysis ◽  
Mouse Embryos ◽  
Wild Type ◽  
Mutant Mouse Embryos

Heightened activation of embryonic megakaryocytes causes aneurysms in the developing brain of mice lacking podoplanin

Blood ◽  
2021 ◽  
Author(s):  
Christopher Michael Hoover ◽  
Yuji Kondo ◽  
Bojing Shao ◽  
Michael McDaniel ◽  
Robert Lee ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Platelet Activation ◽  
Mutant Mouse ◽  
Neural Tissue ◽  
Mouse Embryos ◽  
Wild Type ◽  
Primitive Hematopoiesis ◽  
Mutant Mouse Embryos ◽  
Granule Release ◽  
Angiopoietin 1

During early embryonic development in mammals, including humans and mice, megakaryocytes first originate from primitive hematopoiesis in the yolk sac. These embryonic megakaryocytes (eMk) circulate in the vasculature with unclear function. Here we report that podoplanin (PDPN), the ligand of C-type lectin-like receptor (CLEC-2) on megakaryocytes/platelets, is temporarily expressed in neural tissue during midgestation in mice. Loss of PDPN or CLEC-2 resulted in aneurysms and spontaneous hemorrhage specifically in the lower diencephalon during midgestation. Surprisingly, more eMks/platelets had enhanced granule release and localized to lower diencephalon in mutant mouse embryos than wild-type littermates prior to hemorrhage. We found that PDPN counteracted the collagen I-induced secretion of angiopoietin-1 from fetal megakaryocytes, which coincided with enhanced TIE2 activation in aneurysm-like sprouts of PDPN-deficient embryos. Blocking platelet activation prevented the PDPN-deficient embryo from developing vascular defects. Our data reveal a new role for PDPN in regulating eMk function during midgestation.

scholarly journals Analysis of HeyL expression in wild-type and Notch pathway mutant mouse embryos

2000 ◽  
Vol 98 (1-2) ◽  
pp. 175-178 ◽  
Author(s):  
Cornelia Leimeister ◽  
Nina Schumacher ◽  
Christian Steidl ◽  
Manfred Gessler
Keyword(s):  
Mutant Mouse ◽  
Notch Pathway ◽  
Mouse Embryos ◽  
Wild Type ◽  
Mutant Mouse Embryos

scholarly journals Morphology, topology and dimensions of the heart and arteries of genetically normal and mutant mouse embryos at stages S21-S23

2017 ◽  
Vol 231 (4) ◽  
pp. 600-614 ◽  
Author(s):  
Stefan H. Geyer ◽  
Lukas F. Reissig ◽  
Markus Hüsemann ◽  
Cordula Höfle ◽  
Robert Wilson ◽  
...  
Keyword(s):  
Mutant Mouse ◽  
Mouse Embryos ◽  
Mutant Mouse Embryos

scholarly journals Anterior neural plate regionalization in cripto null mutant mouse embryos in the absence of node and primitive streak

2003 ◽  
Vol 264 (2) ◽  
pp. 537-549 ◽  
Author(s):  
Giovanna L Liguori ◽  
Diego Echevarría ◽  
Raffaele Improta ◽  
Massimo Signore ◽  
Eileen Adamson ◽  
...  
Keyword(s):  
Mutant Mouse ◽  
Primitive Streak ◽  
Neural Plate ◽  
Mouse Embryos ◽  
Null Mutant ◽  
Mutant Mouse Embryos ◽  
Anterior Neural Plate ◽  
Null Mutant Mouse

The behaviour in vitro of epidermal cells from normal and pupoid foetus mutant mouse embryos

1985 ◽  
Vol 79 (1) ◽  
pp. 305-315
Author(s):  
S. Anderson ◽  
D.A. Ede
Keyword(s):  
Mutant Mouse ◽  
Cell Mass ◽  
Time Lapse ◽  
Epidermal Cells ◽  
Mouse Embryos ◽  
Sensory Nerves ◽  
Recessive Lethal Mutation ◽  
Mutant Mouse Embryos ◽  
Peripheral Sensory

Pupoid foetus (pf/pf) is a recessive lethal mutation of the mouse causing epidermal hypertrophy and disorganization of peripheral sensory nerves and mesoderm. The comparative behaviour of epidermal cells from normal and pupoid foetus mutant mouse embryos was studied in vitro. Epidermal explants from the snout region of 12.5- to 13-day embryos were grown in culture for periods of up to 2 weeks. Cultures from both phenotypes were filmed using time-lapse cinemicrography for up to 3 days following explantation. Paths of individual cells were traced as they migrated from the explant, and their rate of locomotion and directional persistence were calculated. Differences in these parameters between the two phenotypes were tested statistically. The overall morphology of the cultures, and the tendency of the cells to detach from the periphery of the cell mass were also compared. The results show that, between 25 and 60 h after explantation, epidermal cells from pupoid foetus embryos move consistently more slowly than normal cells, and follow a more erratic path. This situation is reversed, however, between 16 and 25 h. This suggests that the pf mutation causes an alteration in epidermal cells that affects their locomotion, and which is maintained for a minimum of 3 days in the absence of other influencing factors.

Control of somite number in normal and amputated mutant mouse embryos: an experimental and a theoretical analysis

Development ◽  
1978 ◽  
Vol 45 (1) ◽  
pp. 189-202
Author(s):  
O. P. Flint ◽  
D. A. Ede ◽  
O. K. Wilby ◽  
J. Proctor
Keyword(s):  
Quantitative Analysis ◽  
Theoretical Analysis ◽  
Mutant Mouse ◽  
Mouse Embryos ◽  
Mammalian Embryo ◽  
Size Number ◽  
Mutant Mouse Embryos ◽  
Recessive Mutant ◽  
Post Implantation ◽  
Somite Number

A regulation is shown for size and number of serially repeated axial structures, the somites, in a mammalian embryo. The mammalian embryo is normally inaccessible to operation at post-implantation stages. This problem is resolved by the quantitative analysis of somite size, number and development in a recessive mutant of the mouse, amputated, whose axial length is greatly reduced. The effect of the gene simulates an experiment ablating part of the embryonic tissue available for somitic segmentation. Regulation occurs at the time when the somite is first formed, by control of the quantity of cells included in each new somite. A model is devised for the control of somitic segmentation which explains most of the features observed and which can be simulated on a computer.

scholarly journals Microarray analysis of Foxa2 mutant mouse embryos reveals novel gene expression and inductive roles for the gastrula organizer and its derivatives

BMC Genomics ◽  
2008 ◽  
Vol 9 (1) ◽  
pp. 511 ◽  
Author(s):  
Owen J Tamplin ◽  
Doris Kinzel ◽  
Brian J Cox ◽  
Christine E Bell ◽  
Janet Rossant ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Mutant Mouse ◽  
Mouse Embryos ◽  
Novel Gene ◽  
Mutant Mouse Embryos
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