Repeated Sequence Target Sites for Maternal DNA-Binding Proteins in Genes Activated in Early Sea Urchin Development

1994 ◽  
Vol 163 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Roger Anderson ◽  
Roy J. Britten ◽  
Eric H. Davidson
Keyword(s):  
Dna Binding ◽  
Sea Urchin ◽  
Binding Proteins ◽  
Repeated Sequence ◽  
Dna Binding Proteins ◽  
Target Sites

South Western blot mapping: A procedure for simultaneous characterization of DNA binding proteins and their specific genomic DNA target sites

1989 ◽  
Vol 179 (2) ◽  
pp. 299-303 ◽  
Author(s):  
Jean-Claude Lelong ◽  
Grégoire Prevost ◽  
Kyong il Lee ◽  
Michel Crepin
Keyword(s):  
Dna Binding ◽  
Western Blot ◽  
Genomic Dna ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Target Sites

scholarly journals How DNA-Binding Proteins find their Target Sites in Human Cells

2013 ◽  
Vol 104 (2) ◽  
pp. 541a-542a
Author(s):  
Davide Normanno ◽  
Lydia Boudaréne ◽  
Claire Dugast-Darzacq ◽  
Xavier Darzacq ◽  
Maxime Dahan
Keyword(s):  
Dna Binding ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Human Cells ◽  
Target Sites

Cloning of two sea urchin DNA-binding proteins involved in mitochondrial DNA replication and transcription

Gene ◽  
2002 ◽  
Vol 286 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Paola Loguercio Polosa ◽  
Fiammetta Megli ◽  
Barbara Di Ponzio ◽  
Maria Nicola Gadaleta ◽  
Palmiro Cantatore ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Dna Binding ◽  
Sea Urchin ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Mitochondrial Dna Replication

scholarly journals Collaborative Competition Mechanism for Gene Activation In Vivo

2003 ◽  
Vol 23 (5) ◽  
pp. 1623-1632 ◽  
Author(s):  
Joanna A. Miller ◽  
Jonathan Widom
Keyword(s):  
Dna Binding ◽  
Binding Proteins ◽  
Regulatory Protein ◽  
Gene Activation ◽  
Dna Binding Proteins ◽  
Regulatory Proteins ◽  
Target Sites ◽  
Collaborative Competition

ABSTRACT The mechanism by which gene regulatory proteins gain access to their DNA target sites is not known. In vitro, binding is inherently cooperative between arbitrary DNA binding proteins whose target sites are located within the same nucleosome. We refer to such competition-based cooperativity as collaborative competition. Here we show that arbitrarily chosen foreign DNA binding proteins, LexA and Tet repressor, cooperate with an adjacently binding endogenous activator protein, Gcn4, to coactivate expression of chromosomal reporter genes in Saccharomyces cerevisiae. Coactivation requires that the cooperating target sites be within a nucleosome-length distance; it leads to increased occupancy by Gcn4 at its binding site; and it requires both Gcn5 and Swi/Snf which, at an endogenous Gcn4-dependent promoter, act subsequent to Gcn4 binding. These results imply that collaborative competition contributes to gene regulation in vivo. They further imply that, even in the presence of the cell's full wild-type complement of chromatin remodeling factors, competition of regulatory proteins with histone octamer for access to regulatory target sites remains a quantitative determinant of gene expression levels. We speculate that initial target site recognition and binding may occur via spontaneous nucleosomal site exposure, with remodeling factor action required downstream to lock in higher levels of regulatory protein occupancy.

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