Extraction and Characterization of the Insecticidal Toxin Hirsutellin A Produced by Hirsutella thompsonii var. thompsonii

1995 ◽  
Vol 19 (4) ◽  
pp. 254-262 ◽  
Author(s):  
Wei-Zhen Liu ◽  
Drion G. Boucias ◽  
Clayton W. McCoy
2014 ◽  
Vol 10 (8) ◽  
pp. e1004288 ◽  
Author(s):  
Wen-Jen Chen ◽  
Feng-Chia Hsieh ◽  
Fu-Chiun Hsu ◽  
Yi-Fang Tasy ◽  
Je-Ruei Liu ◽  
...  
Keyword(s):  

Toxins ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 2024-2040 ◽  
Author(s):  
Ihsan Ullah ◽  
Eun-Kyung Jang ◽  
Min-Sung Kim ◽  
Jin-Ho Shin ◽  
Gun-Seok Park ◽  
...  

2010 ◽  
Vol 58 (23) ◽  
pp. 12343-12349 ◽  
Author(s):  
Je-Ruei Liu ◽  
Yung-Di Lin ◽  
Shying-Tzu Chang ◽  
Yi-Fang Zeng ◽  
San-Lan Wang

Toxins ◽  
2018 ◽  
Vol 10 (6) ◽  
pp. 233
Author(s):  
Zhen Xiao ◽  
Yunxiao Zhang ◽  
Jiao Zeng ◽  
Songping Liang ◽  
Cheng Tang ◽  
...  

2017 ◽  
Vol 398 (1) ◽  
pp. 135-142 ◽  
Author(s):  
Miriam Olombrada ◽  
Pilar Medina ◽  
Flor Budia ◽  
José G. Gavilanes ◽  
Álvaro Martínez-del-Pozo ◽  
...  

Abstract Metarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against ribosomes and suggested as potential insecticides. Here we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are of interest.


Toxicon ◽  
2007 ◽  
Vol 50 (6) ◽  
pp. 861-867 ◽  
Author(s):  
Nobuto Matsushita ◽  
Masahiro Miyashita ◽  
Atsushi Sakai ◽  
Yoshiaki Nakagawa ◽  
Hisashi Miyagawa

Toxins ◽  
2014 ◽  
Vol 6 (5) ◽  
pp. 1490-1504 ◽  
Author(s):  
Leopoldo Palma ◽  
Delia Muñoz ◽  
Colin Berry ◽  
Jesús Murillo ◽  
Primitivo Caballero

Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.


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