Activated (HLA-DR+) T-Lymphocyte Subsets in Cervical Carcinoma and Effects of Radiotherapy and Immunotherapy with Sizofiran on Cell-Mediated Immunity and Survival

To explore the antitumor activity of crude polysaccharides of Dioscorea bulbifera L. (DBLP) and its possible mechanism, the effects of DBLP on the tumor growth in vivo, thymus and spleen weight were examined. Apoptosis of thymus, spleen and tumor tissue in tumor-bearing mice was detected by the flow cytometry. The number of CD4+ and CD8+ T-lymphocyte subsets of peripheral blood was also determined by means of FACScan flow cytometer. Our results showed that U14 cervical carcinoma growth could be inhibited, thymus weight be increased, apoptosis of thymus and spleen be decreased, and apoptosis of tumor be increased by DBLP in tumor-bearing mice. Furthermore, DBLP had the abilities to reduce the ratio of CD4+/CD8+ T-lymphocyte according to CTX group. These results indicated that DBLP could suppress the cervical carcinoma through modulating immune response of the tumor-bearing mice and inducing apoptosis of tumor tissue.

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Immunologic Monitoring of T-Lymphocyte Subsets and HLA-DR-Positive Monocytes in Kidney Transplant Recipients

Medicine ◽

10.1097/01.md.0000479946.86789.21 ◽

2016 ◽

Vol 95 (3) ◽

pp. e8921

Keyword(s):

Kidney Transplant ◽

T Lymphocyte ◽

Lymphocyte Subsets ◽

Transplant Recipients ◽

Kidney Transplant Recipients ◽

T Lymphocyte Subsets ◽

Hla Dr ◽

Immunologic Monitoring

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T-Lymphocyte Subsets and HLA-DR-Expressing Cells in the Osteoarthritic Synovialis

Scandinavian Journal of Rheumatology ◽

10.3109/03009748509100403 ◽

1985 ◽

Vol 14 (3) ◽

pp. 259-264 ◽

Cited By ~ 17

Author(s):

Olof Johnell ◽

Anders Hulth ◽

Anders Henricson

Keyword(s):

T Lymphocyte ◽

Lymphocyte Subsets ◽

T Lymphocyte Subsets ◽

Hla Dr

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Application of immunoelectron microscopy for identification of T lymphocyte subsets and HLA DR antigen positive cells in tissue.

Journal of Histochemistry & Cytochemistry ◽

10.1177/32.5.6232311 ◽

1984 ◽

Vol 32 (5) ◽

pp. 547-551 ◽

Cited By ~ 14

Author(s):

H P Dienes ◽

K Essling ◽

H D John

Keyword(s):

T Lymphocyte ◽

Lymphocyte Subsets ◽

Suppressor Cells ◽

T Cell Subsets ◽

Electron Microscopic Level ◽

Electron Microscopic ◽

T Lymphocyte Subsets ◽

Minimal Loss ◽

Hla Dr ◽

Cryostat Sections

The identification of T lymphocyte subsets by means of monoclonal antisera in tissue has so far been restricted to light microscopic observations on cryostat sections, since the conventional fixatives as formaldehyde, B5, or glutaraldehyde seem to denature the surface antigens. Applying a mild fixative, periodate-lysine-paraformaldehyde (PLP), we were able to demonstrate T cell subsets on the electron microscopic level in human tonsils with minimal loss of antigenicity of the surface markers Leu 1, OKT4, OKT8, and HLA DR. The preservation of the tissue could be compared favorably to fixation with glutaraldehyde, and provided an ultrastructural basis to distinguish helper and suppressor cells by morphological features as well.

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