scholarly journals Application of immunoelectron microscopy for identification of T lymphocyte subsets and HLA DR antigen positive cells in tissue.

1984 ◽  
Vol 32 (5) ◽  
pp. 547-551 ◽  
Author(s):  
H P Dienes ◽  
K Essling ◽  
H D John
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Suppressor Cells ◽  
T Cell Subsets ◽  
Electron Microscopic Level ◽  
Electron Microscopic ◽  
T Lymphocyte Subsets ◽  
Minimal Loss ◽  
Hla Dr ◽  
Cryostat Sections

The identification of T lymphocyte subsets by means of monoclonal antisera in tissue has so far been restricted to light microscopic observations on cryostat sections, since the conventional fixatives as formaldehyde, B5, or glutaraldehyde seem to denature the surface antigens. Applying a mild fixative, periodate-lysine-paraformaldehyde (PLP), we were able to demonstrate T cell subsets on the electron microscopic level in human tonsils with minimal loss of antigenicity of the surface markers Leu 1, OKT4, OKT8, and HLA DR. The preservation of the tissue could be compared favorably to fixation with glutaraldehyde, and provided an ultrastructural basis to distinguish helper and suppressor cells by morphological features as well.

Immunopathological patterns in the rectal mucosa of patients with shigellosis: expression of HLA-DR antigens and T-lymphocyte subsets

Apmis ◽  
1994 ◽  
Vol 102 (1-6) ◽  
pp. 371-380 ◽  
Author(s):  
RUBHANA Raqib ◽  
FINN P. Reinholt ◽  
PRADEEP KUMAR Bardhan ◽  
ANDERS KÄRnell ◽  
ALF A. Lindberg
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Rectal Mucosa ◽  
T Lymphocyte Subsets ◽  
Hla Dr

scholarly journals Immunologic Monitoring of T-Lymphocyte Subsets and HLA-DR-Positive Monocytes in Kidney Transplant Recipients

Medicine ◽  
2015 ◽  
Vol 94 (44) ◽  
pp. e1902 ◽  
Author(s):  
Jang-Hee Cho ◽  
Young-Deuk Yoon ◽  
Hye Min Jang ◽  
Eugene Kwon ◽  
Hee-Yeon Jung ◽  
...  
Keyword(s):  
Kidney Transplant ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
T Lymphocyte Subsets ◽  
Hla Dr ◽  
Immunologic Monitoring

IN VIVO BIODOSIMETRY OF PORCINE T-LYMPHOCYTE SUBSETS AND NK CELLS

2019 ◽  
Vol 186 (2-3) ◽  
pp. 181-185
Author(s):  
Lenka Andrejsová ◽  
Zuzana Šinkorová ◽  
Jiří Šinkora ◽  
Aleš Tichý ◽  
Alžběta Filipová ◽  
...  
Keyword(s):  
Nk Cells ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Peripheral Blood Lymphocytes ◽  
T Cell Subsets ◽  
T Helper ◽  
Large White ◽  
T Lymphocyte Subsets ◽  
Significant Difference

Abstract The aim of the present study was to evaluate the biodosimetric potential of peripheral blood lymphocytes, particularly of T-cell subsets (null and T helper) and natural killer cells (NK), upon exposure to gamma irradiation (60Co) in vivo. For this purpose, the change in relative numbers of NK cells and T-lymphocyte subsets, as well as in the H2AX phosphorylation rate, were evaluated as potential early markers of the lymphocytic response to irradiation in vivo. These experiments were performed on a Large White Pig model. As a result, significant but not dose-dependent changes in the proportion of lymphocyte subpopulations (NK cells, null and T helper cells) were found after exposure to ionising radiation in vivo. On the other hand, circulating NK cells showed relatively higher radioresistance capacity when compared to the T-lymphocyte subsets; however, gamma-H2AX expression showed no significant difference between the evaluated lymphocyte subsets.

Activated (HLA-DR+) T-Lymphocyte Subsets in Cervical Carcinoma and Effects of Radiotherapy and Immunotherapy with Sizofiran on Cell-Mediated Immunity and Survival

1995 ◽  
Vol 56 (3) ◽  
pp. 412-420 ◽  
Author(s):  
Kohji Miyazaki ◽  
Hiroshi Mizutani ◽  
Hidetaka Katabuchi ◽  
Keizo Fukuma ◽  
Shunichi Fujisaka ◽  
...  
Keyword(s):  
Cervical Carcinoma ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Cell Mediated Immunity ◽  
T Lymphocyte Subsets ◽  
Hla Dr

Activated (HLA-DR+) T-Lymphocyte Subsets in Early Epithelial Ovarian Cancer and Malignant Ovarian Germ Cell Tumors

1995 ◽  
Vol 58 (3) ◽  
pp. 362-367 ◽  
Author(s):  
K. Miyazaki ◽  
K. Shimada ◽  
H. Katabuchi ◽  
F. Arakane ◽  
S. Arao ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Germ Cell ◽  
Epithelial Ovarian Cancer ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Germ Cell Tumors ◽  
T Lymphocyte Subsets ◽  
Hla Dr

scholarly journals Clinical Investigations. T-Lymphocyte Subset Absolute Counts in the Peripheral Blood of Mediterranean Spotted Fever Patients: Relations to Disease Severity / Абсолютное Количество Субпопуляций Т-Лимфоцитов В Перифери- Ческой Крови Пациентов Со Средиземноморской Пятнистой Лихо- Радкой: Установление Связи С Тяжестью Заболевания

Folia Medica ◽  
2015 ◽  
Vol 57 (2) ◽  
pp. 93-103 ◽  
Author(s):  
Ivan G. Baltadzhiev ◽  
Pavel I. Pavlov
Keyword(s):  
T Cell ◽  
Disease Severity ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
Spotted Fever ◽  
Lymphocyte Subset ◽  
T Cell Subsets ◽  
Mediterranean Spotted Fever ◽  
T Lymphocyte Subsets ◽  
The Absolute

AbstractINTRODUCTION: Mediterranean spotted fever (MSF) in Bulgaria is caused by Rickettsia conorii conorii. Aim: This study aims at investigating the absolute counts of T-lymphocyte subsets in the peripheral blood of patients with MSF in order to establish relationships with disease severity. MATERIALS AND METHODS: The absolute counts of T-lymphocyte subsets were tested in the blood of 62 patients in the acute stage of MSF. They were assigned into three age and sex matched groups, based on the severity of disease - with mild, moderate or severe forms. Controls were 32 age and sex matched healthy individuals. The diagnosis was confirmed by an immunofluorescence assay. Immunophenotyping was performed using Epics XL-MCL Coulter, USA flow-cytometer. RESULTS: The absolute counts of immune competent (CD3+) cells, as well as the counts of helper/inducer (CD3+CD4+) and suppressor/ cytotoxic (CD3+CD8+) T-cell subsets decreased in parallel with disease severity. Naïve (CD4+CD45RA+) and activated memory (CD4+CD45RO+) T-cell subsets were reduced, particularly in severe MSF. Taken as a whole, the counts of activated (CD3+HLA-DR+) and that of presenting accessory (CD28+) or stimulatory (CD38+) molecules Т-cell subsets was increased, but in the first two subsets the trend from mild to severe forms of the disease was descending. CONCLUSION: Reduced T-lymphocyte subset counts are likely related to trans-migration into perivascular inflammatory foci. The increased number of T-lymphocytes bearing activation molecules reflects a mobilization of the cell-mediated immune response. An important issue of this study is the possible prognostic value of T-cell subsets counting, predicting the evolution of a clinical condition to clinical forms, according to the disease severity.

scholarly journals Effects of herpes virus carrier status on peripheral T lymphocyte subsets

Blood ◽  
1987 ◽  
Vol 70 (2) ◽  
pp. 516-523 ◽  
Author(s):  
JW Gratama ◽  
AM Naipal ◽  
MA Oosterveer ◽  
T Stijnen ◽  
HC Kluin-Nelemans ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocyte ◽  
Herpes Virus ◽  
Lymphocyte Subsets ◽  
T Cell Subsets ◽  
Carrier Status ◽  
Nonspecific Esterase ◽  
T Lymphocyte Subsets ◽  
Herpès Virus ◽  
Virus Carrier

Abstract We studied the effects of herpes virus carrier status on peripheral blood T lymphocyte subsets in 334 healthy individuals. IgG-class antibodies against cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpes simplex virus (HSV), and varicella-zoster virus (VZV) were used as markers for the carrier status of those viruses. CMV carrier status was associated with significant increases in the numbers of some T cell subsets, whereas the carrier status of EBV, HSV, and VZV had no significant effects. The 159 CMV-seropositive individuals had higher numbers of HNK1+ T cells than did the 175 CMV-seronegative individuals [mean (SD), 292 (196)/microL v 164 (89)/microL, respectively], including the CD4+HNK1+ T cells [38 (48)/microL v 9 (13)/microL, respectively] and the CD8+HNK1+ T cells [166 (146)/microL v 73 (54)/microL, respectively]. Morphological and cytochemical studies showed that the expression of HNK1 by the CD4+ and CD8+ T cells was associated with the occurrence of azurophilic cytoplasmatic granules and a loss of nonspecific esterase activity. The numbers of CD4+HNK1+ and CD8+HNK1+ T cells increased proportionally to the levels of the IgG- class CMV antibody titers. We suggest that the increased numbers of CD4+HNK1+ and CD8+HNK1+ granular T cells in CMV carriers reflect the persistent interaction between CMV and the immune system of its hosts.

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