Critical Role of the Acceptor Stem of tRNAsMet in their Aminoacylation by Escherichia coli Methionyl-tRNA Synthetase

1993 ◽  
Vol 229 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Thierry Meinnel ◽  
Yves Mechulam ◽  
Christine Lazennec ◽  
Sylvain Blanquet ◽  
Guy Fayat
Keyword(s):  
Escherichia Coli ◽  
Critical Role ◽  
Trna Synthetase ◽  
Acceptor Stem ◽  
Methionyl Trna Synthetase

SATP (YaaH), a succinate–acetate transporter protein in Escherichia coli

2013 ◽  
Vol 454 (3) ◽  
pp. 585-595 ◽  
Author(s):  
Joana Sá-Pessoa ◽  
Sandra Paiva ◽  
David Ribas ◽  
Inês Jesus Silva ◽  
Sandra Cristina Viegas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Succinic Acid ◽  
Critical Role ◽  
Amino Acid Residues ◽  
E Coli ◽  
Transporter Protein ◽  
Affinity Constants ◽  
In Trans

In the present paper we describe a new carboxylic acid transporter in Escherichia coli encoded by the gene yaaH. In contrast to what had been described for other YaaH family members, the E. coli transporter is highly specific for acetic acid (a monocarboxylate) and for succinic acid (a dicarboxylate), with affinity constants at pH 6.0 of 1.24±0.13 mM for acetic acid and 1.18±0.10 mM for succinic acid. In glucose-grown cells the ΔyaaH mutant is compromised for the uptake of both labelled acetic and succinic acids. YaaH, together with ActP, described previously as an acetate transporter, affect the use of acetic acid as sole carbon and energy source. Both genes have to be deleted simultaneously to abolish acetate transport. The uptake of acetate and succinate was restored when yaaH was expressed in trans in ΔyaaH ΔactP cells. We also demonstrate the critical role of YaaH amino acid residues Leu131 and Ala164 on the enhanced ability to transport lactate. Owing to its functional role in acetate and succinate uptake we propose its assignment as SatP: the Succinate–Acetate Transporter Protein.

scholarly journals Use of β3-methionine as an amino acid substrate of Escherichia coli methionyl-tRNA synthetase

2020 ◽  
Vol 209 (2) ◽  
pp. 107435 ◽  
Author(s):  
Giuliano Nigro ◽  
Sophie Bourcier ◽  
Christine Lazennec-Schurdevin ◽  
Emmanuelle Schmitt ◽  
Philippe Marlière ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Trna Synthetase ◽  
Methionyl Trna Synthetase ◽  
Acid Substrate ◽  
Amino Acid Substrate

scholarly journals Methionyl-tRNA Synthetase from Escherichia coli

2005 ◽  
Vol 127 (3) ◽  
pp. 449-457 ◽  
Author(s):  
David G. BARKER ◽  
Jean-Pierre EBEL ◽  
Ross JAKES ◽  
Chris J. BRUTON
Keyword(s):  
Escherichia Coli ◽  
Trna Synthetase ◽  
Methionyl Trna Synthetase
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