Understanding the mechanism of ice binding by type III antifreeze proteins

2001 ◽  
Vol 305 (4) ◽  
pp. 875-889 ◽  
Author(s):  
Alfred A. Antson ◽  
Derek J. Smith ◽  
David I. Roper ◽  
Sally Lewis ◽  
Leo S.D. Caves ◽  
...  
Keyword(s):  
Antifreeze Proteins ◽  
Type Iii ◽  
Ice Binding

scholarly journals Ice-binding site of surface-bound type III antifreeze protein partially decoupled from water

2018 ◽  
Vol 20 (42) ◽  
pp. 26926-26933 ◽  
Author(s):  
Dominique Verreault ◽  
Sarah Alamdari ◽  
Steven J. Roeters ◽  
Ravindra Pandey ◽  
Jim Pfaendtner ◽  
...  
Keyword(s):  
Binding Site ◽  
Antifreeze Proteins ◽  
Antifreeze Protein ◽  
Water Interface ◽  
Native State ◽  
Type Iii ◽  
Ice Binding ◽  
Air Water Interface

Combined SFG/MD analysis together with spectral calculations revealed that type III antifreeze proteins adsorbed at the air–water interface maintains a native state and adopts an orientation that leads to a partial decoupling of its ice-binding site from water.

scholarly journals Molecular basis of ice-binding and cryopreservation activities of type III antifreeze proteins

2021 ◽  
Vol 19 ◽  
pp. 897-909
Author(s):  
Seo-Ree Choi ◽  
Jaewang Lee ◽  
Yeo-Jin Seo ◽  
Hyun Sun Kong ◽  
Minjae Kim ◽  
...  
Keyword(s):  
Molecular Basis ◽  
Antifreeze Proteins ◽  
Type Iii ◽  
Ice Binding

Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout

Cryobiology ◽  
2008 ◽  
Vol 57 (1) ◽  
pp. 46-51 ◽  
Author(s):  
Yu Hirano ◽  
Yoshiyuki Nishimiya ◽  
Shuichiro Matsumoto ◽  
Michiaki Matsushita ◽  
Satoru Todo ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Antifreeze Proteins ◽  
Type Iii ◽  
Hypothermic Preservation

scholarly journals Janus effect of antifreeze proteins on ice nucleation

2016 ◽  
Vol 113 (51) ◽  
pp. 14739-14744 ◽  
Author(s):  
Kai Liu ◽  
Chunlei Wang ◽  
Ji Ma ◽  
Guosheng Shi ◽  
Xi Yao ◽  
...  
Keyword(s):  
Liquid Water ◽  
Molecular Level ◽  
Simulation Analysis ◽  
Antifreeze Proteins ◽  
Freezing Temperature ◽  
Ice Nucleation ◽  
Dynamics Simulation ◽  
Ice Binding ◽  
Solid Substrates ◽  
Unique Capability

The mechanism of ice nucleation at the molecular level remains largely unknown. Nature endows antifreeze proteins (AFPs) with the unique capability of controlling ice formation. However, the effect of AFPs on ice nucleation has been under debate. Here we report the observation of both depression and promotion effects of AFPs on ice nucleation via selectively binding the ice-binding face (IBF) and the non–ice-binding face (NIBF) of AFPs to solid substrates. Freezing temperature and delay time assays show that ice nucleation is depressed with the NIBF exposed to liquid water, whereas ice nucleation is facilitated with the IBF exposed to liquid water. The generality of this Janus effect is verified by investigating three representative AFPs. Molecular dynamics simulation analysis shows that the Janus effect can be established by the distinct structures of the hydration layer around IBF and NIBF. Our work greatly enhances the understanding of the mechanism of AFPs at the molecular level and brings insights to the fundamentals of heterogeneous ice nucleation.

Hydration Shell of Antifreeze Proteins: Unveiling the Role of Non-Ice-Binding Surfaces

2019 ◽  
Vol 123 (30) ◽  
pp. 6474-6480 ◽  
Author(s):  
Laura Zanetti-Polzi ◽  
Akash Deep Biswas ◽  
Sara Del Galdo ◽  
Vincenzo Barone ◽  
Isabella Daidone
Keyword(s):  
Antifreeze Proteins ◽  
Hydration Shell ◽  
Ice Binding

scholarly journals A peek at ice binding by antifreeze proteins

2011 ◽  
Vol 108 (18) ◽  
pp. 7281-7282 ◽  
Author(s):  
K. A. Sharp
Keyword(s):  
Antifreeze Proteins ◽  
Ice Binding

Multivalent Display of Antifreeze Proteins by Fusion to Self-Assembling Protein Cages Enhances Ice-Binding Activities

Biochemistry ◽  
2016 ◽  
Vol 55 (49) ◽  
pp. 6811-6820 ◽  
Author(s):  
Sean W. Phippen ◽  
Corey A. Stevens ◽  
Tyler D. R. Vance ◽  
Neil P. King ◽  
David Baker ◽  
...  
Keyword(s):  
Antifreeze Proteins ◽  
Protein Cages ◽  
Self Assembling ◽  
Ice Binding ◽  
Multivalent Display

scholarly journals Ice-binding mechanism of winter flounder antifreeze proteins

1997 ◽  
Vol 73 (6) ◽  
pp. 2851-2873 ◽  
Author(s):  
A. Cheng ◽  
K.M. Merz
Keyword(s):  
Antifreeze Proteins ◽  
Winter Flounder ◽  
Binding Mechanism ◽  
Ice Binding
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