Comparative Studies of Phase-Cycling Schemes for Multiple π-Pulse Sequences

1997 ◽  
Vol 127 (2) ◽  
pp. 144-146 ◽  
Author(s):  
Tatyana I. Igumenova ◽  
Dan J. Mitchell ◽  
Jeremy N.S. Evans
2020 ◽  
Vol 22 (9) ◽  
pp. 092001
Author(s):  
Andreas Wituschek ◽  
Oleg Kornilov ◽  
Tobias Witting ◽  
Laura Maikowski ◽  
Frank Stienkemeier ◽  
...  

2002 ◽  
Vol 16 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Youlin Xia ◽  
Kong Hung Sze ◽  
Ning Li ◽  
Pang Chui Shaw ◽  
Guang Zhu

Protein dynamics can be characterized by relaxation parameters obtained from traditional 2D HSQC based NMR experiments. This approach is hampered when applied to proteins with severe spectral overlap. In the present work, several novel 3D TROSY-HNCO and 3D HSQC-HNCO based NMR experiments were applied for measuring15NT1,T2and1H-15N NOE with improved spectral dispersion by introducing a third13C dimension. The number of phase cycling steps in these 3D pulse sequences was restricted to two in order to minimize the time required to perform the dynamics measurements. For a uniformly 100%15N, 100%13C, and 70%2H-labelled trichosanthin sample (~27 kDa, 1.0 mM) at 30°C, the sensitivity of 3D TROSY-HNCO based experiment was, on the average, enhanced by 72% compared to that of 3D HSQC-HNCO based experiments. However, the 3D HSQC-HNCO based experiments should be more effective for non-deuterated proteins with smaller molecular weights and seriously overlapped 2D HSQC spectra. Results from the 3D TROSY-HNCO and 3D HSQC-HNCO based experiments were in good agreement with those obtained from traditional 2D HSQC based experiments.


1950 ◽  
Vol 16 (1) ◽  
pp. 91-103 ◽  
Author(s):  
John A. Layne ◽  
F.R. Schemm ◽  
W.W. Hurst

1972 ◽  
Vol 5 (1) ◽  
pp. 306-309
Author(s):  
Arthur J. O'Shea ◽  
Mervin D. Lynch ◽  
Thomas F. Harrington
Keyword(s):  

2001 ◽  
Vol 30 (5) ◽  
pp. 276-284 ◽  
Author(s):  
M Sakamoto ◽  
T Sasano ◽  
S Higano ◽  
S Takahashi ◽  
T Nagasaka ◽  
...  

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