Spectracular: minimizing spectral overlap in multicolor flow cytometry experiments

Designing multicolor antibody panels for flow cytometry is complex and time-consuming. Poorly designed panels can result in repeated rounds of optimization and irreproducible results due to spectral overlap between fluorochromes. However, few tools exist to help researchers when designing panels, and none automatically considers spectral overlap. Here we present Spectracular, a web server which utilizes a complexity optimization algorithm to suggest fluorochrome combinations with minimal or near-minimal spectral overlap. Spectracular is available at https://biosurf.org/spectracular.html.

Time-resolved analysis of photoluminescence at a single wavelength for ratiometric and multiplex biosensing and bioimaging

Simultaneous analysis of luminescence signals of multiple probes can improve the accuracy and efficiency of biosensing and bioimaging. Analysis of multiple signals at different wavelengths usually suffers from spectral overlap,...

A biocompatible dual-AIEgen system without spectral overlap for quantitation of microbial viability and monitoring of biofilm formation

A dual-AIEgen system for microbial imaging and metabolic status sensing has been realized through chemistry strategies. This dual-AIEgen system can detect general microbes and identify their viabilities as well as their microbial biofilms.

Time-absorbance profile ratio background correction: introducing TAP to correct for spectral overlap in high-resolution continuum source graphite furnace atomic absorption spectrometry

A new method to correct for spectral overlap when using HR CS GFAAS is proposed.

Excited States Engineering Enables Efficient Near-Infrared Lasing in Nanographenes

The spectral overlap between stimulated emission (SE) and absorption from dark states (i.e. charges and triplets) especially in the near-infrared (NIR), represents one of the most effective gain loss channel...