scholarly journals 1300. Lentiviral Vectors Utilizing the Chicken β-Globin 5′HS4 Insulator and the Human Interferon-α Scaffold Attachment Region for High Level and Sustained Factor VIII Gene Expression

2002 ◽  
Vol 5 (5) ◽  
pp. S425 ◽  
Keyword(s):  
Gene Expression ◽  
Factor Viii ◽  
Lentiviral Vectors ◽  
Human Interferon ◽  
Interferon Α ◽  
Factor Viii Gene ◽  
Attachment Region ◽  
Scaffold Attachment Region ◽  
High Level ◽  
Human Interferon Α

83 EFFECTS OF HUMAN INTERFERON-α ON GENE EXPRESSION IN THE BOVINE ENDOMETRIUM IN COMPARISON TO DAYS 15 AND 18 OF PREGNANCY

2012 ◽  
Vol 24 (1) ◽  
pp. 154
Author(s):  
S. Bauersachs ◽  
S. E. Ulbrich ◽  
H. D. Reichenbach ◽  
M. Reichenbach ◽  
M. Büttner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endometrial Tissue ◽  
Differentially Expressed ◽  
Human Interferon ◽  
Control Group ◽  
Interferon Α ◽  
Type I ◽  
Fatty Acid Binding ◽  
General Role ◽  
Human Interferon Α

Interferon-τ (IFNT), a Type-I interferon (IFN), is the pregnancy recognition signal produced by the ruminant conceptus (Godkin et al. 1984; Hansen et al. 1985; Helmer et al. 1987; Spencer et al. 2007). In addition to these specific functions of IFNT in ruminants, many studies suggest that IFNs play a general role in establishment of pregnancy and conceptus attachment/implantation in most mammalian species (Bazer et al. 2009; Bazer et al. 2010; Johnson et al. 2009; Roberts et al. 2008). To characterise the effects of prototype Type-I IFNs on bovine endometrium, in experiment one, Simmental heifers were treated from Day 14 to Day 16 of the oestrous cycle with a rod-shaped intrauterine device releasing human interferon-α (IFNA) or placebo lipid extrudates or PBS only as controls (n = 4 each). Lipid formulation and concentration of human IFNA were adjusted to release 8–9 × 107 IU of IFNA over a period of 2 days in in vitro release experiments. On Day 16, endometrial biopsy samples were collected after flushing the uterus. In experiment 2, endometrial tissue samples were obtained on Day 12, 15 and 18 post-mating from nonpregnant or pregnant heifers. All samples from both experiments were analysed with an Affymetrix Bovine Genome Array (Santa Clara, CA). In experiment one, IFNA treatment resulted in differential gene expression in the bovine endometrium. Significant differences were found between the IFNA group and both control groups, whereas no differences were observed between the placebo and the PBS control group. In experiment 2, differentially expressed genes were found between pregnant and nonpregnant endometria on Day 15 and 18, but not on Day 12, with many of them known IFN-stimulated genes. The comparison of the data sets from both experiments showed very similar gene expression changes for most of the typical IFN-stimulated genes. In addition, several genes were identified which were differentially expressed after IFNA treatment but not different at Day 15 or 18 of pregnancy compared with nonpregnant animals. Conversely, some genes were found as differentially expressed during pregnancy but not after IFNA treatment. Differential expression of selected genes was verified by quantitative real-time PCR and 4 genes, namely jumonji C domain containing histone demethylase 1 homologue D (JHDM1D), indoleamine 2,3-dioxygenase 1 (IDO1), fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor) (FABP3) and dickkopf homologue 1 (DKK1), were selected for localization of mRNA expression in endometrial tissue sections. The findings of this study suggest that there may be differential effects of bovine IFNT compared with human IFNA and that some pregnancy-specific changes in the endometrium are elicited by conceptus-derived factors other than IFNT. This study was supported by the German Ministry for Education and Research (BMBF, FUGATO-plus, COMPENDIUM) and the German Research Foundation (DFG FOR478). The authors are part of the European Union COST action GEMINI.

scholarly journals A strategy for high-level expression of soluble and functional human interferon α as a GST-fusion protein in E.coli

2007 ◽  
Vol 20 (5) ◽  
pp. 201-209 ◽  
Author(s):  
Imen Rabhi-Essafi ◽  
Amine Sadok ◽  
Noureddine Khalaf ◽  
Dahmani M. Fathallah
Keyword(s):  
Fusion Protein ◽  
Human Interferon ◽  
Interferon Α ◽  
High Level Expression ◽  
Gst Fusion Protein ◽  
High Level ◽  
Human Interferon Α

A high-level and regulatable production system for recombinant glycoproteins using a human interferon-α promoter-based expression vector

Gene ◽  
1994 ◽  
Vol 144 (2) ◽  
pp. 289-293 ◽  
Author(s):  
Mori Tetsuya ◽  
Yamamoto Kozo ◽  
Ohta Tsunetaka ◽  
Sakamoto Chiyo ◽  
Sato Michiyo ◽  
...  
Keyword(s):  
Production System ◽  
Expression Vector ◽  
Human Interferon ◽  
Interferon Α ◽  
Recombinant Glycoproteins ◽  
High Level ◽  
Human Interferon Α

Detection of human interferon-α-encoding gene expression

Gene ◽  
1993 ◽  
Vol 127 (2) ◽  
pp. 261-266 ◽  
Author(s):  
Evelyn R. Brandt ◽  
Rodney J. Devenish ◽  
Brian F. Cheetham ◽  
Anthony W. Linnane
Keyword(s):  
Gene Expression ◽  
Human Interferon ◽  
Interferon Α ◽  
Encoding Gene ◽  
Human Interferon Α

scholarly journals Generation of transgenic fibroblasts producing doxycycline-inducible human interferon-α or erythropoietin for a bovine mammary bioreactor

2012 ◽  
Vol 12 (1) ◽  
pp. 1137-1144 ◽  
Author(s):  
HEE YOUNG KANG ◽  
EUI-JU HONG ◽  
KYU-CHAN HWANG ◽  
NAM-HYUNG KIM ◽  
WOO-SUK HWANG ◽  
...  
Keyword(s):  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Long-term stabilization of recombinant human interferon α 2b in aqueous solution without serum albumin

2003 ◽  
Vol 264 (1-2) ◽  
pp. 57-72 ◽  
Author(s):  
Llamil Ruiz ◽  
Nuria Reyes ◽  
Ladys Duany ◽  
Abrisleyda Franco ◽  
Kethia Aroche ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Serum Albumin ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α

Human interferon-α induces immobility in the mouse forced swimming test: involvement of the opioid system

2000 ◽  
Vol 852 (2) ◽  
pp. 482-484 ◽  
Author(s):  
Mitsuhiro Makino ◽  
Yutaka Kitano ◽  
Chika Komiyama ◽  
Masaaki Hirohashi ◽  
Morihiro Kohno ◽  
...  
Keyword(s):  
Forced Swimming Test ◽  
Forced Swimming ◽  
Opioid System ◽  
Human Interferon ◽  
Interferon Α ◽  
Swimming Test ◽  
Human Interferon Α

A genetic study of human interferon-α-induced repair of DNA damage in hepatitis B patients

1991 ◽  
Vol 262 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Peilin Wang ◽  
Shuying Cong ◽  
Xiaoyong Dong ◽  
Zaichun Chen ◽  
Chuiping Ma
Keyword(s):  
Dna Damage ◽  
Hepatitis B ◽  
Genetic Study ◽  
Human Interferon ◽  
Interferon Α ◽  
Human Interferon Α
Sign in / Sign up

Export Citation Format

Share Document