Isolation and Characterization of 26- and 30-kDa Rat Liver Proteins Immunoreactive to Anti-Sterol Carrier Protein-2 Antibodies

1998 ◽  
Vol 13 (3) ◽  
pp. 337-348 ◽  
Author(s):  
Lixia Pu ◽  
William B. Foxworth ◽  
Ann B. Kier ◽  
Roland S. Annan ◽  
Steven A. Carr ◽  
...  
Keyword(s):  
Rat Liver ◽  
Carrier Protein ◽  
Sterol Carrier Protein ◽  
Isolation And Characterization ◽  
Sterol Carrier Protein 2

scholarly journals Subcellular localization of sterol carrier protein-2 in rat hepatocytes: its primary localization to peroxisomes.

1989 ◽  
Vol 108 (4) ◽  
pp. 1353-1361 ◽  
Author(s):  
G A Keller ◽  
T J Scallen ◽  
D Clarke ◽  
P A Maher ◽  
S K Krisans ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Rat Liver ◽  
Polyclonal Antibodies ◽  
Rat Hepatocytes ◽  
Carrier Protein ◽  
Sterol Carrier Protein ◽  
Rat Liver Cells ◽  
Ultrathin Frozen Sections ◽  
Sterol Carrier Protein 2

Sterol carrier protein-2 (SCP-2) is a nonenzymatic protein of 13.5 kD which has been shown in in vitro experiments to be required for several stages in cholesterol utilization and biosynthesis. The subcellular localization of SCP-2 has not been definitively established. Using affinity-purified rabbit polyclonal antibodies against electrophoretically pure SCP-2 from rat liver, we demonstrate by immunoelectron microscopic labeling of ultrathin frozen sections of rat liver that the largest concentration of SCP-2 is inside peroxisomes. In addition the immunolabeling indicates that there are significant concentrations of SCP-2 inside mitochondria, and associated with the endoplasmic reticulum and the cytosol, but not inside the Golgi apparatus, lysosomes, or the nucleus. These results were confirmed by immunoblotting experiments with proteins from purified subcellular fractions of the rat liver cells carried out with the anti-SCP-2 antibodies. The large concentration of SCP-2 inside peroxisomes strongly supports the proposal that peroxisomes are critical sites of cholesterol utilization and biosynthesis. The presence of SCP-2 inside peroxisomes and mitochondria raises questions about the mechanisms involved in the differential targeting of SCP-2 to these organelles.

scholarly journals Primary sequence and structural analysis of sterol carrier protein 2 from rat liver: homology with immunoglobulins.

1987 ◽  
Vol 262 (27) ◽  
pp. 13219-13227 ◽  
Author(s):  
A Pastuszyn ◽  
B J Noland ◽  
J F Bazan ◽  
R J Fletterick ◽  
T J Scallen
Keyword(s):  
Structural Analysis ◽  
Rat Liver ◽  
Carrier Protein ◽  
Sterol Carrier Protein ◽  
Primary Sequence ◽  
Sterol Carrier Protein 2

scholarly journals Characterization of the yellow fever mosquito sterol carrier protein-2 like 3 gene and ligand-bound protein structure

2009 ◽  
Vol 326 (1-2) ◽  
pp. 67-77 ◽  
Author(s):  
David H. Dyer ◽  
Irina Vyazunova ◽  
Jeffery M. Lorch ◽  
Katrina T. Forest ◽  
Que Lan
Keyword(s):  
Protein Structure ◽  
Yellow Fever ◽  
Carrier Protein ◽  
Sterol Carrier Protein ◽  
Yellow Fever Mosquito ◽  
Sterol Carrier Protein 2

Elevated cholesterol and decreased sterol carrier protein-2 in peroxisomes from AS-30D hepatoma compared to normal rat liver

1991 ◽  
Vol 285 (2) ◽  
pp. 238-245 ◽  
Author(s):  
H.T. Lyons ◽  
A. Kharroubi ◽  
N. Wolins ◽  
Scott Tenner ◽  
R.F. Chanderbhan ◽  
...  
Keyword(s):  
Rat Liver ◽  
Carrier Protein ◽  
Sterol Carrier Protein ◽  
Elevated Cholesterol ◽  
Normal Rat ◽  
Sterol Carrier Protein 2
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