Production and Purification of a Recombinant Human hsp60 Epitope Using the Cellulose-Binding Domain inEscherichia coli

1998 ◽  
Vol 14 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Etai Shpigel ◽  
Dana Elias ◽  
Irun R. Cohen ◽  
Oded Shoseyov
Keyword(s):  
Binding Domain ◽  
Inescherichia Coli ◽  
Cellulose Binding Domain ◽  
Cellulose Binding ◽  
Human Hsp60

The cellulose-binding domain (CBDCex) of an exoglucanase fromCellulomonas fimi: Production inEscherichia coli and characterization of the polypeptide

1993 ◽  
Vol 42 (4) ◽  
pp. 401-409 ◽  
Author(s):  
Edgar Ong ◽  
Neil R. Gilkes ◽  
Robert C. Miller ◽  
R. Antony J. Warren ◽  
Douglas G. Kilburn
Keyword(s):  
Binding Domain ◽  
Inescherichia Coli ◽  
Cellulose Binding Domain ◽  
Cellulose Binding

scholarly journals Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor–cellulose-binding domain fusion protein

1999 ◽  
Vol 339 (2) ◽  
pp. 429-434
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fusion Protein ◽  
Stem Cell Factor ◽  
Direct Analysis ◽  
Binding Domain ◽  
Target Cells ◽  
Cellulose Binding Domain ◽  
Binding Characteristics ◽  
Cellulose Binding ◽  
Cellulose Surface

A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF–CBDCex is up to 7-fold more potent than soluble SCF–CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF–CBDCex, activated receptors and SCF–CBDCex co-localize on the cellulose matrix. The strong binding of SCF–CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.

scholarly journals Fusion Endoglucanase Cel12B from Thermotoga maritima with Cellulose Binding Domain

BioResources ◽  
2018 ◽  
Vol 13 (2) ◽  
Author(s):  
Hao Shi ◽  
Yanling Chen ◽  
Wenjian Peng ◽  
Pixiang Wang ◽  
Yuping Zhao ◽  
...  
Keyword(s):  
Thermotoga Maritima ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulose Binding

scholarly journals Acetyl xylan esterase II fromPenicillium purpurogenumis similar to an esterase fromTrichoderma reeseibut lacks a cellulose binding domain

FEBS Letters ◽  
1998 ◽  
Vol 423 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Rodrigo Gutiérrez ◽  
Ella Cederlund ◽  
Lars Hjelmqvist ◽  
Alessandra Peirano ◽  
Francisco Herrera ◽  
...  
Keyword(s):  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Acetyl Xylan Esterase ◽  
Cellulose Binding

Fabrication of cotton fabrics using family III cellulose-binding domain for enhanced surface properties

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105202-105205 ◽  
Author(s):  
Liting Zhang ◽  
Yaofei Sun ◽  
Wenji Yao ◽  
Guoying Dai ◽  
Ping Wang
Keyword(s):  
Surface Properties ◽  
Cotton Fabric ◽  
Surface Functionalization ◽  
Physical Adsorption ◽  
Cotton Fabrics ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Fabric Surface ◽  
Cellulose Binding ◽  
Enhanced Surface

Cotton fabric surface functionalization by physical adsorption of CBDIII through a sample soaking process.

scholarly journals Probing the role of tryptophan residues in a cellulose-binding domain by chemical modification

1996 ◽  
Vol 5 (11) ◽  
pp. 2311-2318 ◽  
Author(s):  
Mark R. Bray ◽  
Neil R. Gilkes ◽  
Douglas G. Kilburn ◽  
R. Antony J. Warren ◽  
Lawrence P. Mcintosh ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Tryptophan Residues ◽  
Cellulose Binding
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