scholarly journals Sequence Parameters That Determine Specificity of Binding of the Replication-Associated Protein to Its Cognate Site in Two Strains of Tomato Leaf Curl Virus–New Delhi

Virology ◽  
2000 ◽  
Vol 273 (2) ◽  
pp. 341-350 ◽  
Author(s):  
Anju Chatterji ◽  
Udayan Chatterji ◽  
Roger N. Beachy ◽  
Claude M. Fauquet
Keyword(s):  
Tomato Leaf ◽  
New Delhi ◽  
Tomato Leaf Curl Virus ◽  
Specificity Of Binding ◽  
Sequence Parameters ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals First Report of Association of Tomato leaf curl virus-New Delhi with Yellow Mosaic Disease of Luffa cylindrica in India

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
S. S. Sohrab ◽  
B. Mandal ◽  
R. P. Pant ◽  
A. Varma
Keyword(s):  
Mosaic Disease ◽  
Tomato Leaf ◽  
New Delhi ◽  
Luffa Cylindrica ◽  
Yellow Mosaic Disease ◽  
Tomato Leaf Curl Virus ◽  
Sponge Gourd ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Sponge gourd (Luffa cylindrica), an important cucurbitaceous vegetable in India, is affected by a disease (2) causing yellow spots on newly emerged leaves, mosaic, mild leaf curling and distortion, small leaves, and misshapen fruits. Nearly 100% of sponge gourd plants were symptomatic in Delhi. Geminivirus-like particles were observed with electron microscopy of uranyl acetate-stained leaf-dip preparations of the diseased plants collected from experimental fields at the Indian Agricultural Research Institute in New Delhi during May and June of 2002. The virus was transmitted by the whitefly (Bemisia tabaci) to sponge and ridge gourd (L. acutangula) after an acquisition and inoculation access period of 24 h each. Whitefly-inoculated plants produced typical yellow mosaic symptoms and contained geminate particles. Nucleic acid extracted from the field-infected and experimentally infected plants hybridized with 32P-labeled probe to DNA-A of Indian cassava mosaic virus, suggesting association of a begomovirus. The viral DNA, isolated by the alkali denaturation method (1) from the experimentally infected sponge gourd plants, was cloned in pBS SK+ at the EcoRI site. A clone with an insert of 2,658 bp was sequenced (GenBank Accession Nos. AJ557219, AJ555488, and AY309957) which shared 89.6 to 95.1% identity with the DNA-A of different strains of Tomato leaf curl virus-New Delhi (ToLCV-NDe). The highest sequence identity (95.1%) was with the severe strain of ToLCV-NDe (GenBank Accession No. U15015). The data suggest that the begomovirus associated with the yellow mosaic disease of L. cylindrica in India is a putative strain of ToLCV-NDe. Reference: (1) K. M. Srivastava et al. J. Virol. Methods 51:297, 1995. (2) A. Varma and B. K. Giri. Virus diseases. Pages 225–245 in: Cucurbits. N. M. Nayar and T. A More, eds. Oxford and IBH Publishing House Private Ltd., New Delhi, India, 1998.

scholarly journals ​Molecular Characterization of Viruses Associated with Leaf Curl Symptoms on Capsicum (Bell Pepper) under Protected Cultivations in Dharwad, Karnataka

2021 ◽  
Author(s):  
C. Channakeshava. M.S. Patil. B. Gurupad ◽  
N.B. Moger
Keyword(s):  
Tomato Leaf ◽  
Bell Pepper ◽  
Ammonium Bromide ◽  
New Delhi ◽  
Tomato Leaf Curl Virus ◽  
Study Results ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Chilli Leaf Curl Virus ◽  
Leaf Curl

Background: Leaf curl or yellowing symptoms, typical of those caused by begomovirus infection, are commonly observed in capsicum (bell pepper) plants in polyhouses. Three capsicum samples with leaf curl and yellowing symptoms were collected from polyhouse at Hi-tech Horticulture, University of Agricultural Sciences, Dharwad, Karnataka, India, during 2017-2018. Methods: Total nucleic acid was isolated from symptomatic and non-symptomatic samples by following CTAB (Cetyl trimethyl ammonium bromide) method and PCR amplified using degenerate and coat protein gene primers of chilli and tomato leaf curl virus. The virus was confirmed by gel electrophoresis with 0.8 per cent agarose. Amplified PCR product was eluted and sent to sequencing (Chromous biotech Pvt. Ltd., Bengaluru). Result: The primer pair could amplify only Chilli leaf curl virus at ~500 bp but not Tomato leaf curl virus. Sequence homology of ChiLCV isolate form Dharwad matched with ChiLCV-Papaya-New Delhi (HM14036), ChiLCV-Chilli-Jodhpu (HM007104) and ChiLCV-Chilli-Noida (HM007114) respectively. The present study results showed that Chilli leaf curl virus is associated with the leaf curl and yellowing symptoms on capsicum under protected cultivation in Dharwad, Karnataka, India.

scholarly journals Identification of Replication Specificity Determinants in Two Strains of Tomato Leaf Curl Virus from New Delhi

1999 ◽  
Vol 73 (7) ◽  
pp. 5481-5489 ◽  
Author(s):  
Anju Chatterji ◽  
Malla Padidam ◽  
Roger N. Beachy ◽  
Claude M. Fauquet
Keyword(s):  
Binding Site ◽  
Tomato Leaf ◽  
Single Amino Acid ◽  
New Delhi ◽  
Rep Protein ◽  
Tomato Leaf Curl Virus ◽  
Severe Strain ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

ABSTRACT We used two strains of tomato leaf curl virus from New Delhi to investigate specificity in replication of their cognate genomes. The strains share 94% sequence identity and are referred to as severe and mild on the basis of symptoms on tomato and tobacco. Replication assays in tobacco protoplasts and plants showed that a single amino acid change, Asn10 to Asp in the N terminus of Rep protein, determines specificity for replication of the two strains based upon its interaction with the origin of replication (ori) sequences. The change of Asp10 to Asn in Rep protein of the mild strain coupled with point mutations at the 3rd and 10th nucleotides of the 13-mer binding site altered its replication ability, resulting in increased levels of virus accumulation. Similarly, changing Asn10 to Asp in Rep protein of the severe strain impaired replication of the virus and altered its severe phenotype in plants. Site-directed mutations made inori and Asn10 of Rep protein suggested that Asn10 recognizes the third base pair of the putative binding site sequence GGTGTCGGAGTC in the severe strain.

Use of Tomato leaf curl virus (TYLCV) truncated Rep gene sequence to engineer TYLCV resistance in tomato plants

2009 ◽  
Vol 53 (2) ◽  
pp. 99-104 ◽  
Author(s):  
H. Tamarzizt ◽  
S. Chouchane ◽  
R. Lengliz ◽  
D. Maxwell ◽  
M. Marrakchi ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Tomato Leaf ◽  
Tomato Plants ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Evidence for recombination among the tomato leaf curl virus strains/species from Bangalore, India

2002 ◽  
Vol 147 (2) ◽  
pp. 255-272 ◽  
Author(s):  
N. Kirthi ◽  
S. P. Maiya ◽  
M. R. N. Murthy ◽  
H. S. Savithri
Keyword(s):  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Virus Strains ◽  
Leaf Curl

scholarly journals Molecular Characterization of a New Tomato Begomovirus from India

Plant Disease ◽  
2003 ◽  
Vol 87 (5) ◽  
pp. 598-598 ◽  
Author(s):  
S. L. Shih ◽  
W. S. Tsai ◽  
S. K. Green ◽  
P. M. Hanson ◽  
G. B. Valand ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Western India ◽  
Breeding Lines ◽  
Tomato Leaf Curl Virus ◽  
Northern India ◽  
Tomato Breeding ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Virus Isolates ◽  
Leaf Curl

The Asian Vegetable Research and Development Center's (AVRDC) tomato breeding lines derived from Lycopersicon hirsutum f. glabratum B 6013 × L. esculentum H-24 and carrying the Ty-2 resistance gene located on chromosome 11 are tolerant to tomato leaf curl disease in Karnataka State, southern India (3), where several isolates of Tomato leaf curl Virus-Bangalore (GenBank Accession Nos. L11746, Z48182, and AF165098) and Tomato leaf curl virus-Karnataka (GenBank Accession No. U38239) are reported to infect tomatoes. The only area in south and southeast Asia where these AVRDC tomato breeding lines were found susceptible to begomovirus infection is Thailand, where several bipartite Tomato yellow leaf curl virus isolates (GenBank Accession Nos. X63015, X63016; AF141922, AF141897; and AF511529, AF511528) are reported to be prevalent. However, in field trials conducted in the fall of 1999 in Bodeli, Gujarat State, western India, the AVRDC breeding lines showed typical symptoms of begomovirus infection, such as leaf curling and vein clearing. The presence of a different tomato begomovirus was suspected. Viral DNA from a symptomatic plant from Bodeli was amplified by polymerase chain reaction (PCR) using the begomovirus-specific degenerate primer pair PAL1v1978/PAR1c715 (4) and the expected 1.4-kb PCR product was obtained. Based on the sequence of the 1.4-kb DNA product, specific primers were designed to complete the DNA-A sequence. The DNA-A of the virus associated with tomato leaf curl from Bodeli consists of 2,759 nucleotides (GenBank Accession No. AF413671) and contains six open reading frames (ORFs V1, V2, C1, C2, C3, and C4). The DNA-A sequence of the Bodeli isolate had highest sequence identities of 98 and 98.3%, respectively, with viruses causing tomato leaf curl from Varanasi, Uttar Pradesh State, northern India (GenBank Accession No. AF449999) collected in the fall of 1999 and Panchkhal, Nepal (GenBank Accession No. AY234383) collected in early 2000. There was no evidence for the presence of DNA-B in the Bodeli, Panchkhal, or Varanasi virus isolates using DNA-B specific primer pairs DNABLC1/DNABLV2 and DNABLC2/DNABLV2 (2). However, a 1.3-kb DNA-beta was detected in the Panchkhal and Varanasi isolates using the primer pair Beta01/Beta02 (1). Sequence comparisons with begomovirus sequences available in the GenBank database showed that these three virus isolates and GenBank Accession No. AY190290 collected in 2001 from Varanasi shared more than 97% sequence identity with each other and should be considered closely related strains of the same virus. These four virus isolates belong to a new distinct tomato geminivirus species because their sequences share less than 88% sequence identities with the next most closely related virus, Tomato leaf curl virus-Karnataka (GenBank Accession No. U38239). This new tomato leaf curl virus is prevalent in western India, northern India, and Nepal. References: (1) R. W. Briddon et al. Mol. Biotechnol. 20:315, 2002. (2) S. K. Green et al. Plant Dis. 85:1286, 2001. (3) V. Muniyappa et al. HortScience 37:603, 2002. (4) M. R. Rojas et al. Plant Dis. 77:340, 1993.

scholarly journals Effects of Tomato Leaf Curl Virus on Growth and Yield Parameters of Tomato Crop

2021 ◽  
Vol 39 (1) ◽  
pp. 79-83
Author(s):  
Yasir Iftikhar ◽  
◽  
Mustansar Mubeen ◽  
Ashara Sajid ◽  
Mohamed Ahmad Zeshan ◽  
...  
Keyword(s):  
Disease Severity ◽  
Disease Incidence ◽  
Tomato Leaf ◽  
Growth And Yield ◽  
Tomato Crop ◽  
Tomato Leaf Curl Virus ◽  
Yield Parameters ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Iftikhar, Y., M. Mubeen, A. Sajid, M.A. Zeshan, Q. Shakeel, A. Abbas, S. Bashir, M. Kamran and H. Anwaar. 2021. Effects of Tomato Leaf Curl Virus on Growth and Yield Parameters of Tomato Crop. Arab Journal of Plant Protection, 39(1): 79-83. Tomato is an important vegetable crop, belongs to the family Solanaceae and is the second most consumed vegetable following potatoes. The tomato crop is grown all over the world in both summer and winter seasons, and plant viruses are a major threat to tomato production. Among these viruses, tomato leaf curl virus (TLCV) causes considerable yield loss to tomato crop. This virus is transmitted by a whitefly (Bemisia tabaci) vector. In this study, the effect of TLCV infection, on the following tomato growth and yield parameters, was evaluated: plant leaf number and area, plant biomass, plant height, root length, and plant stem diameter and yield. Tomato plants were transplanted in wellprepared plots with 4 replications. The control group was covered with polyethene bag to avoid whitefly infestation. Plants were scored on the 15th and 30th day after inoculation and TLCV disease severity was recorded. Analysis of variance (ANOVA) showed the significant differences between the healthy and infected tomato plants. Moreover, growth and yield parameters were reduced with the increase in disease incidence, disease severity and whitefly infestation. Disease severity was increased with the increase in temperature during the growing season. It can be concluded from this study that TLCV significantly affects growth and yield of the tomato crop. Keywords: Tomato, Tomato leaf curl virus, TLCV, disease incidence, disease severity.

scholarly journals EFFECT OF ENVIRONMENTAL FACTORS ON BIOCHEMICAL PROPERTIES OF TOMATO LEAF CURL VIRUS INFECTED LEAVES OF TOMATO

2019 ◽  
Vol 31 (1) ◽  
pp. 105-111
Author(s):  
Saneela Arooj ◽  
Yasir Iftekhar ◽  
Mustansar Mubeen ◽  
Muhammad I. Ullah ◽  
Ashara Sajid ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Biochemical Properties ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Silencing of tomato CTR1 provides enhanced tolerance against Tomato leaf curl virus infection

2019 ◽  
Vol 14 (3) ◽  
pp. e1565595 ◽  
Author(s):  
Ravindra K. Chandan ◽  
Achuit K. Singh ◽  
Sunita Patel ◽  
Durga Madhab Swain ◽  
Narendra Tuteja ◽  
...  
Keyword(s):  
Virus Infection ◽  
Tomato Leaf ◽  
Tomato Leaf Curl Virus ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl
Sign in / Sign up

Export Citation Format

Share Document