Leaf Rust Resistance Gene LR34 is Involved in Powdery Mildew Resistance of Cimmyt Bread Wheat Line Saar

Author(s):  
M. Lillemo ◽  
R. P. Singh ◽  
J. Huerta-Espino ◽  
X. M. Chen ◽  
Z. H. He ◽  
...  
Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 655-664 ◽  
Author(s):  
Li Huang ◽  
Steven A Brooks ◽  
Wanlong Li ◽  
John P Fellers ◽  
Harold N Trick ◽  
...  

Abstract We report the map-based cloning of the leaf rust resistance gene Lr21, previously mapped to a generich region at the distal end of chromosome arm 1DS of bread wheat (Triticum aestivum L.). Molecular cloning of Lr21 was facilitated by diploid/polyploid shuttle mapping strategy. Cloning of Lr21 was confirmed by genetic transformation and by a stably inherited resistance phenotype in transgenic plants. Lr21 spans 4318 bp and encodes a 1080-amino-acid protein containing a conserved nucleotide-binding site (NBS) domain, 13 imperfect leucine-rich repeats (LRRs), and a unique 151-amino-acid sequence missing from known NBS-LRR proteins at the N terminus. Fine-structure genetic analysis at the Lr21 locus detected a noncrossover (recombination without exchange of flanking markers) within a 1415-bp region resulting from either a gene conversion tract of at least 191 bp or a double crossover. The successful map-based cloning approach as demonstrated here now opens the door for cloning of many crop-specific agronomic traits located in the gene-rich regions of bread wheat.


Euphytica ◽  
2015 ◽  
Vol 206 (1) ◽  
pp. 57-66
Author(s):  
Ai-yong Qi ◽  
Pei-pei Zhang ◽  
Xian-chun Xia ◽  
Zhong-hu He ◽  
Julio Huerta-Espino ◽  
...  

2008 ◽  
Vol 117 (7) ◽  
pp. 1069-1075 ◽  
Author(s):  
X. L. Zhao ◽  
T. C. Zheng ◽  
X. C. Xia ◽  
Z. H. He ◽  
D. Q. Liu ◽  
...  

2012 ◽  
Vol 91 (2) ◽  
pp. 155-161 ◽  
Author(s):  
AMANDEEP KAUR RIAR ◽  
SATINDER KAUR ◽  
H. S. DHALIWAL ◽  
KULDEEP SINGH ◽  
PARVEEN CHHUNEJA

2015 ◽  
Vol 75 (3) ◽  
pp. 301
Author(s):  
Han Liusha ◽  
Li Zaifeng ◽  
Wang Jiazhen ◽  
Shi Lingzhi ◽  
Zhu Lin ◽  
...  

2014 ◽  
Vol 33 (4) ◽  
pp. 931-937 ◽  
Author(s):  
Lifang Xing ◽  
Cuifen Wang ◽  
Xianchun Xia ◽  
Zhonghu He ◽  
Wanquan Chen ◽  
...  

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