The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge

2007 ◽  
pp. 62-68 ◽  
Author(s):  
D. R. Hall ◽  
S. E. Harding ◽  
D. J. Winzor
Keyword(s):  
Optical System ◽  
Sedimentation Equilibrium ◽  
Low Speed ◽  
Correct Analysis ◽  
Equilibrium Distributions

scholarly journals Analysis of the molecular size of tomato (Lycopersicon esculentum Mill) fruit polyuronides by gel filtration and low-speed sedimentation equilibrium

1987 ◽  
Vol 245 (2) ◽  
pp. 463-466 ◽  
Author(s):  
G B Seymour ◽  
S E Harding
Keyword(s):  
Cell Wall ◽  
Lycopersicon Esculentum ◽  
Tomato Fruit ◽  
Gel Filtration ◽  
Molecular Size ◽  
Sedimentation Equilibrium ◽  
Low Speed ◽  
Lycopersicon Esculentum Mill ◽  
Wall Structures ◽  
Molecular Size Distribution

The cell-wall structures of tomato (Lycopersicon esculentum Mill) and other fruit are intimately linked with the nature of their polyuronides. Cell-wall polyuronides from unripe and ripe tomato fruit were isolated and purified and their molecular size and molecular-size distributions were compared. It was demonstrated that there is a considerable decrease in the weight-average Mr upon ripening (from 160,000 +/- 10,000 to 96,000 +/- 4000) and a corresponding increase in polydispersity, particularly at the low-Mr end of the distribution. The estimates of polyuronide molecular size and molecular-size distribution were obtained without the need for polyuronide standards of known Mr by using gel-filtration chromatography combined with the absolute method of low-speed sedimentation equilibrium.

scholarly journals Analysis of Sedimentation Equilibrium Distributions Reflecting Nonideal Macromolecular Associations

2000 ◽  
Vol 79 (4) ◽  
pp. 2178-2187 ◽  
Author(s):  
Peter R. Wills ◽  
Michael P. Jacobsen ◽  
Donald J. Winzor
Keyword(s):  
Sedimentation Equilibrium ◽  
Equilibrium Distributions
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