Influence of Roflumilast on Airway Reactivity and Apoptosis in Ovalbumin-Sensitized Guinea Pigs

2014 ◽  
pp. 11-18 ◽  
Author(s):  
I. Medvedova ◽  
M. Prso ◽  
A. Eichlerova ◽  
D. Mokra ◽  
P. Mikolka ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Airway Reactivity

Effects of Selective Inhibition of PDE4 by YM976 on Airway Reactivity and Cough in Ovalbumin-Sensitized Guinea Pigs

2016 ◽  
pp. 61-70 ◽  
Author(s):  
J. Mokrý ◽  
A. Urbanová ◽  
I. Medvedová ◽  
M. Kertys ◽  
P. Mikolka ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Selective Inhibition ◽  
Airway Reactivity

Effects of Selective Inhibition of PDE4 and PDE7 on Airway Reactivity and Cough in Healthy and Ovalbumin-Sensitized Guinea Pigs

2012 ◽  
pp. 57-64 ◽  
Author(s):  
Juraj Mokry ◽  
Marta Joskova ◽  
Daniela Mokra ◽  
Ingrid Christensen ◽  
Gabriela Nosalova
Keyword(s):  
Guinea Pigs ◽  
Selective Inhibition ◽  
Airway Reactivity

Effects of Propranolol and Indomethacin on Muscarinic Airway Reactivity in Unanesthetized Guinea Pigs

1986 ◽  
Vol 181 (4) ◽  
pp. 569-574 ◽  
Author(s):  
J. H. Roum ◽  
C. G. Murlas
Keyword(s):  
Guinea Pigs ◽  
Airway Reactivity

Antagonism of airway reactivity induced by ovalbumin antigen in guinea pigs by 5-amino-4-imidazolecarboxamide riboside

1991 ◽  
Vol 88 (4) ◽  
pp. 604-612 ◽  
Author(s):  
D BERGREN ◽  
R TOWNLEY ◽  
D MARQUARDT ◽  
H GRUBER ◽  
V BERGREN
Keyword(s):  
Guinea Pigs ◽  
Airway Reactivity

Toluene diisocyanate-induced airway hyperreactivity in guinea pigs depleted of granulocytes

1988 ◽  
Vol 64 (5) ◽  
pp. 1773-1778 ◽  
Author(s):  
W. Cibulas ◽  
S. M. Brooks ◽  
C. G. Murlas ◽  
M. L. Miller ◽  
R. T. McKay
Keyword(s):  
Guinea Pigs ◽  
Bronchial Hyperreactivity ◽  
Airway Hyperreactivity ◽  
Toluene Diisocyanate ◽  
High Concentration ◽  
Airway Reactivity ◽  
Cyclophosphamide Treatment ◽  
Tracheal Tissue ◽  
Unanesthetized Animals ◽  
And Control

The influence of cyclophosphamide-induced granulocyte depletion on toluene diisocyanate (TDI)-related changes in airway reactivity and pathology was assessed in guinea pigs. Twelve cyclophosphamide-treated and 12 control animals comprising each group were studied physiologically before and 2 h after a single 10-min exposure to 3 ppm of TDI. Reactivity was determined in intact unanesthetized animals by measuring specific airway conductance before and during intravenous acetylcholine infusion. After testing, tracheal tissue for light microscopic examination was obtained from three hyperreactive guinea pigs in each exposed group and compared with tissue from treated and control animals (n = 3 each) that had not been TDI exposed. Cyclophosphamide treatment caused substantial decreases in both circulating and airway granulocyte counts. However, the incidence and degree of bronchial hyperreactivity that occurred 2 h post-TDI was similar in the untreated and treated groups. Our results indicate that TDI-induced bronchial hyperreactivity 1) occurs shortly after a brief high concentration exposure and 2) appears independent of circulating or airway granulocyte counts.

Inhaled roflumilast and tadalafil supress airway reactivity and inflammation in ovalbumin-sensitized guinea pigs

2016 ◽  
Author(s):  
Juraj Mokry ◽  
Anna Urbanova ◽  
Martin Kertys ◽  
Ivana Medvedova ◽  
Peter Mikolka ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Airway Reactivity

Effect of Sodium Hypochlorite on Specific Airway Resistance and Airway Reactivity in Guinea Pigs

1993 ◽  
Vol 5 (2) ◽  
pp. 251-263 ◽  
Author(s):  
Taro Mizutani ◽  
Takahiro Kobayashi ◽  
Hiroshi Naito
Keyword(s):  
Sodium Hypochlorite ◽  
Guinea Pigs ◽  
Airway Resistance ◽  
Airway Reactivity ◽  
Specific Airway Resistance

scholarly journals Chronic tobacco smoke exposure increases airway sensitivity to capsaicin in awake guinea pigs

2001 ◽  
Vol 90 (2) ◽  
pp. 695-704 ◽  
Author(s):  
Dale R. Bergren
Keyword(s):  
Tobacco Smoke ◽  
Guinea Pigs ◽  
Airway Hyperreactivity ◽  
The Other ◽  
Tobacco Smoke Exposure ◽  
Neurokinin A ◽  
Airway Reactivity ◽  
C Fibers ◽  
P Content ◽  
Neurokinin Antagonists

Tobacco smoke (TS) exposure induces airway hyperreactivity, particularly in sensitive individuals with asthma. However, the mechanism of this airway hyperreactivity is not well understood. To investigate the relative susceptibility of atopic and nonatopic individuals to TS-induced airway hyperreactivity, we exposed ovalbumin (OA)-sensitized and nonsensitized guinea pigs to TS exposure (5 mg/l air, 30-min exposure, 7 days/wk for 120–156 days). Two similar groups exposed to compressed air served as controls. Airway reactivity was assessed as an increase in enhanced pause (Penh) units using a plethysmograph that allowed free movement of the animals. After 90 days of exposure, airway reactivity increased in OA-TS guinea pigs challenged with capsaicin, bradykinin, and neurokinin A fragment 4–10 aerosols. In addition, substance P content increased in lung perfusate of OA-TS guinea pigs in response to acute TS challenge compared with that of the other groups. Airway hyperirritability was not enhanced by phosphoramidon but was attenuated by a cocktail of neurokinin antagonists, nor was airway hyperreactivity observed after either methacholine or histamine challenge in OA-TS guinea pigs. Chronic TS exposure enhanced neither airway reactivity to histamine or methacholine nor contractility of isolated tracheal rings. In conclusion, chronic TS exposure increased airway reactivity to capsaicin and bradykinin aerosol challenge, and OA-TS guinea pigs were most susceptible to airway dysfunction as the result of exposure to TS compared with the other groups. Increased airway reactivity to capsaicin suggests a mechanism involving neurogenic inflammation, such as increased activation of lung C fibers.

scholarly journals Effects of Roflumilast, Selective PDE4 Inhibitor, on Airway Reactivity in Ovalbumin-Sensitized Guinea Pigs

2015 ◽  
Vol 5 (1) ◽  
pp. 12-19
Author(s):  
Nirmathan Tharmalingam ◽  
I. Medvedova ◽  
A. Eichlerova ◽  
M. Prso ◽  
D. Mokra ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Guinea Pigs ◽  
Bronchoalveolar Lavage Fluid ◽  
Inflammatory Cells ◽  
Lavage Fluid ◽  
Whole Body ◽  
Pde4 Inhibitor ◽  
Airway Reactivity

Abstract Background: Roflumilast as a phosphodiesterase 4 inhibitor has shown to increase lung functions and decrease the number of exacerbations in chronic obstructive lung disease. In this study, its ability to decrease the airway hyperresponsiveness in a model of eosinophil inflammation was evaluated. Methods: Healthy adult male guinea pigs were divided into groups as follows: the first group was considered as a healthy control group (without sensitization and therapy), animals in the second group were sensitized with ovalbumin, but left without further treatment, and the animals in the third group were sensitized with ovalbumin and treated with roflumilast perorally for 7 consecutive days. In vivo airway reactivity was evaluated using double-chamber whole body plethysmograph and measuring the specific airway resistance after nebulization of histamine aerosol. In vitro experiments were performed with tissue strips of trachea and lungs in organ bath, where their contractile responses to cumulative doses of acetylcholine and histamine were registered. The numbers of inflammatory cells in blood and bronchoalveolar lavage fluid were measured using standard staining. Results: Guinea pigs with roflumilast treatment showed decreased in vivo and in vitro airway reactivity associated with suppressed recruitment of inflammatory cells (especially eosinophils) in blood and bronchoalveolar lavage fluid. Conclusion: Roflumilast has demonstrated the therapeutic potential in the model of ovalbumin induced eosinophil inflammation typically present in patients with bronchial asthma.

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