Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline

2013 ◽  
pp. 99-109
Author(s):  
Raphaela Rid ◽  
Harald Hundsberger ◽  
Kamil Önder
Keyword(s):  
Transcriptional Profiling ◽  
Primary Keratinocytes ◽  
Compound Screening ◽  
Human Primary Keratinocytes

scholarly journals GATA3 regulates FLG and FLG2 expression in human primary keratinocytes

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jana Zeitvogel ◽  
Neele Jokmin ◽  
Samira Rieker ◽  
Ilona Klug ◽  
Christina Brandenberger ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Human Primary Keratinocytes

scholarly journals Wnt5a/β-Catenin Signaling Drives Calcium-Induced Differentiation of Human Primary Keratinocytes

2014 ◽  
Vol 134 (8) ◽  
pp. 2183-2191 ◽  
Author(s):  
Tanja Popp ◽  
Dirk Steinritz ◽  
Andreas Breit ◽  
Janina Deppe ◽  
Virginia Egea ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Induced Differentiation ◽  
Human Primary Keratinocytes

Isolation and culture of human primary keratinocytes – a methods review

2018 ◽  
Author(s):  
Aneta Ścieżyńska ◽  
Aleksandra Nogowska ◽  
Maria Sikorska ◽  
Justyna Konys ◽  
Anita Karpińska ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Human Primary Keratinocytes

scholarly journals Interferon-Lambda 1 Inhibits Staphylococcus aureus Colonization in Human Primary Keratinocytes

2021 ◽  
Vol 12 ◽  
Author(s):  
Xia Wu ◽  
Yan Zhao ◽  
Ying Gu ◽  
Kun Li ◽  
Xiaojie Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mrna Expression ◽  
Human Keratinocytes ◽  
Skin Lesions ◽  
Inhibitory Effect ◽  
Oxidase Inhibitor ◽  
Primary Keratinocytes ◽  
Intracellular Ros ◽  
Normal Human ◽  
Human Primary Keratinocytes

Atopic dermatitis (AD) is a common inflammatory skin disease. Staphylococcus aureus (S. aureus) colonization in skin lesions occurs in approximately 70% of AD patients. It has been found that IFN-λ1 can inhibit the colonization of S. aureus in normal human nasal mucosa. IFN-λ1 can increase IL-28RA in infected human keratinocytes. In this study, we found that IFN-λ1 can increase mRNA expression of FLG and antimicrobial peptides (AMPs) and inhibit TSLP mRNA expression in infected human keratinocytes. IFN-λ1 can increase intracellular ROS level, decrease STAT1 phosphorylation, and inhibit the colonization of S. aureus in human primary keratinocytes. These effects were attenuated by knocking-down IL-28R and NADPH oxidase inhibitor, suggesting that this function was mediated by JAK-STAT1 signaling pathway. These results suggest that IFN-λ1 might have an inhibitory effect on S. aureus colonization in AD lesions. Our findings might have potential value in the treatment for AD.

scholarly journals Nicotinamide Metabolism Modulates the Proliferation/Differentiation Balance and Senescence of Human Primary Keratinocytes

2019 ◽  
Vol 139 (8) ◽  
pp. 1638-1647.e3 ◽  
Author(s):  
Chye Ling Tan ◽  
Toby Chin ◽  
Christina Yan Ru Tan ◽  
Holly A. Rovito ◽  
Ling Shih Quek ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Human Primary Keratinocytes

scholarly journals Effects of anthrax lethal toxin on human primary keratinocytes

2008 ◽  
Vol 105 (6) ◽  
pp. 1756-1767 ◽  
Author(s):  
S.S. Koçer ◽  
M. Matic ◽  
M. Ingrassia ◽  
S.G. Walker ◽  
E. Roemer ◽  
...  
Keyword(s):  
Lethal Toxin ◽  
Anthrax Lethal Toxin ◽  
Primary Keratinocytes ◽  
Human Primary Keratinocytes

scholarly journals Protocol for the long‐term culture of human primary keratinocytes from the normal colorectal mucosa

2019 ◽  
Vol 234 (7) ◽  
pp. 9895-9905 ◽  
Author(s):  
Elena Torreggiani ◽  
Marika Rossini ◽  
Ilaria Bononi ◽  
Silvia Pietrobon ◽  
Elisa Mazzoni ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Long Term Culture ◽  
Normal Colorectal Mucosa ◽  
Colorectal Mucosa ◽  
Human Primary Keratinocytes

Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion

2016 ◽  
pp. 135-147 ◽  
Author(s):  
Gerhard E. Strittmatter ◽  
Martha Garstkiewicz ◽  
Jennifer Sand ◽  
Serena Grossi ◽  
Hans-Dietmar Beer
Keyword(s):  
Protein Secretion ◽  
Primary Keratinocytes ◽  
Caspase 1 ◽  
Human Primary Keratinocytes
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