Genetic Modification of Human Primary Keratinocytes by Lentiviral Vectors

2019 ◽  
pp. 113-123
Author(s):  
Ana Freije ◽  
Natalia Sanz-Gómez ◽  
Alberto Gandarillas
Keyword(s):  
Genetic Modification ◽  
Lentiviral Vectors ◽  
Primary Keratinocytes ◽  
Human Primary Keratinocytes

scholarly journals GATA3 regulates FLG and FLG2 expression in human primary keratinocytes

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jana Zeitvogel ◽  
Neele Jokmin ◽  
Samira Rieker ◽  
Ilona Klug ◽  
Christina Brandenberger ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Human Primary Keratinocytes

scholarly journals Pseudotyping Lentiviral Vectors: When the Clothes Make the Virus

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1311 ◽  
Author(s):  
Alexis Duvergé ◽  
Matteo Negroni
Keyword(s):  
Gene Therapy ◽  
Genetic Modification ◽  
Viral Vectors ◽  
Molecular Level ◽  
Lentiviral Vectors ◽  
Human Cells ◽  
Surface Proteins ◽  
Holy Grail ◽  
Viral Therapy

Delivering transgenes to human cells through transduction with viral vectors constitutes one of the most encouraging approaches in gene therapy. Lentivirus-derived vectors are among the most promising vectors for these approaches. When the genetic modification of the cell must be performed in vivo, efficient specific transduction of the cell targets of the therapy in the absence of off-targeting constitutes the Holy Grail of gene therapy. For viral therapy, this is largely determined by the characteristics of the surface proteins carried by the vector. In this regard, an important property of lentiviral vectors is the possibility of being pseudotyped by envelopes of other viruses, widening the panel of proteins with which they can be armed. Here, we discuss how this is achieved at the molecular level and what the properties and the potentialities of the different envelope proteins that can be used for pseudotyping these vectors are.

scholarly journals Wnt5a/β-Catenin Signaling Drives Calcium-Induced Differentiation of Human Primary Keratinocytes

2014 ◽  
Vol 134 (8) ◽  
pp. 2183-2191 ◽  
Author(s):  
Tanja Popp ◽  
Dirk Steinritz ◽  
Andreas Breit ◽  
Janina Deppe ◽  
Virginia Egea ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Induced Differentiation ◽  
Human Primary Keratinocytes

Isolation and culture of human primary keratinocytes – a methods review

2018 ◽  
Author(s):  
Aneta Ścieżyńska ◽  
Aleksandra Nogowska ◽  
Maria Sikorska ◽  
Justyna Konys ◽  
Anita Karpińska ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Human Primary Keratinocytes

scholarly journals Interferon-Lambda 1 Inhibits Staphylococcus aureus Colonization in Human Primary Keratinocytes

2021 ◽  
Vol 12 ◽  
Author(s):  
Xia Wu ◽  
Yan Zhao ◽  
Ying Gu ◽  
Kun Li ◽  
Xiaojie Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mrna Expression ◽  
Human Keratinocytes ◽  
Skin Lesions ◽  
Inhibitory Effect ◽  
Oxidase Inhibitor ◽  
Primary Keratinocytes ◽  
Intracellular Ros ◽  
Normal Human ◽  
Human Primary Keratinocytes

Atopic dermatitis (AD) is a common inflammatory skin disease. Staphylococcus aureus (S. aureus) colonization in skin lesions occurs in approximately 70% of AD patients. It has been found that IFN-λ1 can inhibit the colonization of S. aureus in normal human nasal mucosa. IFN-λ1 can increase IL-28RA in infected human keratinocytes. In this study, we found that IFN-λ1 can increase mRNA expression of FLG and antimicrobial peptides (AMPs) and inhibit TSLP mRNA expression in infected human keratinocytes. IFN-λ1 can increase intracellular ROS level, decrease STAT1 phosphorylation, and inhibit the colonization of S. aureus in human primary keratinocytes. These effects were attenuated by knocking-down IL-28R and NADPH oxidase inhibitor, suggesting that this function was mediated by JAK-STAT1 signaling pathway. These results suggest that IFN-λ1 might have an inhibitory effect on S. aureus colonization in AD lesions. Our findings might have potential value in the treatment for AD.

scholarly journals Nicotinamide Metabolism Modulates the Proliferation/Differentiation Balance and Senescence of Human Primary Keratinocytes

2019 ◽  
Vol 139 (8) ◽  
pp. 1638-1647.e3 ◽  
Author(s):  
Chye Ling Tan ◽  
Toby Chin ◽  
Christina Yan Ru Tan ◽  
Holly A. Rovito ◽  
Ling Shih Quek ◽  
...  
Keyword(s):  
Primary Keratinocytes ◽  
Human Primary Keratinocytes
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