Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells

2018 ◽  
pp. 307-321 ◽  
Author(s):  
Tsutomu Motohashi ◽  
Takahiro Kunisada
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Direct Conversion ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts

scholarly journals Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells

Biology Open ◽  
2016 ◽  
Vol 5 (3) ◽  
pp. 311-322 ◽  
Author(s):  
Tsutomu Motohashi ◽  
Natsuki Watanabe ◽  
Masahiro Nishioka ◽  
Yuhki Nakatake ◽  
Piao Yulan ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Neural Crest ◽  
Gene Array ◽  
Neural Crest Cells ◽  
Direct Conversion ◽  
Array Analysis ◽  
Embryonic Fibroblasts ◽  
Gene Array Analysis

Sox10 Functions as an Inducer of the Direct Conversion of Keratinocytes Into Neural Crest Cells

2020 ◽  
Vol 29 (23) ◽  
pp. 1510-1519
Author(s):  
Tsutomu Motohashi ◽  
Norito Kawamura ◽  
Natsuki Watanabe ◽  
Daisuke Kitagawa ◽  
Naoki Goshima ◽  
...  
Keyword(s):  
Neural Crest ◽  
Neural Crest Cells ◽  
Direct Conversion

scholarly journals Nonviral Direct Conversion of Primary Mouse Embryonic Fibroblasts to Neuronal Cells

2012 ◽  
Vol 1 ◽  
pp. e32 ◽  
Author(s):  
Andrew F Adler ◽  
Christopher L Grigsby ◽  
Karina Kulangara ◽  
Hong Wang ◽  
Ryohei Yasuda ◽  
...  
Keyword(s):  
Neuronal Cells ◽  
Direct Conversion ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Primary Mouse

scholarly journals Cell-Permeable Oct4 Gene Delivery Enhances Stem Cell-like Properties of Mouse Embryonic Fibroblasts

2021 ◽  
Vol 22 (17) ◽  
pp. 9357
Author(s):  
Da Hyeon Choi ◽  
Kyeong Eun Lee ◽  
Jiwon Park ◽  
Yoon Jeong Park ◽  
Jue-Yeon Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Epithelial Mesenchymal Transition ◽  
Somatic Cells ◽  
N:P Ratio ◽  
Direct Conversion ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts ◽  
Mesenchymal Transition ◽  
Mesenchymal To Epithelial Transition ◽  
Gene Complexes

Direct conversion of one cell type into another is a trans-differentiation process. Recent advances in fibroblast research revealed that epithelial cells can give rise to fibroblasts by epithelial-mesenchymal transition. Conversely, fibroblasts can also give rise to epithelia by undergoing a mesenchymal to epithelial transition. To elicit stem cell-like properties in fibroblasts, the Oct4 transcription factor acts as a master transcriptional regulator for reprogramming somatic cells. Notably, the production of gene complexes with cell-permeable peptides, such as low-molecular-weight protamine (LMWP), was proposed to induce reprogramming without cytotoxicity and genomic mutation. We designed a complex with non-cytotoxic LMWP to prevent the degradation of Oct4 and revealed that the positively charged cell-permeable LMWP helped condense the size of the Oct4-LMWP complexes (1:5 N:P ratio). When the Oct4-LMWP complex was delivered into mouse embryonic fibroblasts (MEFs), stemness-related gene expression increased while fibroblast intrinsic properties decreased. We believe that the Oct4-LMWP complex developed in this study can be used to reprogram terminally differentiated somatic cells or convert them into stem cell-like cells without risk of cell death, improving the stemness level and stability of existing direct conversion techniques.

scholarly journals Brief exposure to small molecules allows induction of mouse embryonic fibroblasts into neural crest-like precursors

FEBS Letters ◽  
2017 ◽  
Vol 591 (4) ◽  
pp. 590-602 ◽  
Author(s):  
Yuzo Takayama ◽  
Tamami Wakabayashi ◽  
Hiroko Kushige ◽  
Yutaka Saito ◽  
Yoichiro Shibuya ◽  
...  
Keyword(s):  
Neural Crest ◽  
Small Molecules ◽  
Mouse Embryonic Fibroblasts ◽  
Embryonic Fibroblasts

Induction of melanocyte precursors from neural crest cells surrounding the neural tube-like structures developed in vitro using mouse ES cell culture

2007 ◽  
Vol 27 (1) ◽  
pp. 45-52
Author(s):  
Koh-ichi Atoh ◽  
Manae S. Kurokawa ◽  
Hideshi Yoshikawa ◽  
Chieko Masuda ◽  
Erika Takada ◽  
...  
Keyword(s):  
Cell Culture ◽  
Neural Crest ◽  
Neural Tube ◽  
Neural Crest Cells ◽  
Es Cell ◽  
Mouse Es Cell
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