Multiphoton Microscopy for Noninvasive and Label-Free Imaging of Human Skin and Oral Mucosa Equivalents

2019 ◽  
pp. 195-212 ◽  
Author(s):  
Gopu Sriram ◽  
Thankiah Sudhaharan ◽  
Graham D. Wright
Keyword(s):  
Human Skin ◽  
Oral Mucosa ◽  
Multiphoton Microscopy ◽  
Label Free

scholarly journals In Vivo Multiphoton Microscopy for Investigating Biomechanical Properties of Human Skin

2010 ◽  
Vol 4 (2) ◽  
pp. 231-238 ◽  
Author(s):  
Xing Liang ◽  
Benedikt W. Graf ◽  
Stephen A. Boppart
Keyword(s):  
Human Skin ◽  
Multiphoton Microscopy ◽  
Biomechanical Properties

Label-free imaging of spinal cords injured tissues with multiphoton microscopy

2021 ◽  
Author(s):  
Xiwen Chen ◽  
Shuang Wang ◽  
Jianxin Chen
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free

In-vivo multiphoton microscopy (MPM) of laser-induced optical breakdown (LIOB) in human skin (Conference Presentation)

2017 ◽  
Author(s):  
Mihaela Balu ◽  
Griffin Lentsch ◽  
Dorota Korta ◽  
Karsten Konig ◽  
Kristen M. Kelly ◽  
...  
Keyword(s):  
Human Skin ◽  
Multiphoton Microscopy ◽  
Optical Breakdown

scholarly journals Label-free multiphoton microscopy reveals altered tissue architecture in hippocampal sclerosis

Epilepsia ◽  
2016 ◽  
Vol 58 (1) ◽  
pp. e1-e5 ◽  
Author(s):  
Ortrud Uckermann ◽  
Roberta Galli ◽  
Susann Leupold ◽  
Roland Coras ◽  
Matthias Meinhardt ◽  
...  
Keyword(s):  
Hippocampal Sclerosis ◽  
Multiphoton Microscopy ◽  
Label Free ◽  
Tissue Architecture

scholarly journals Differential influence of Streptococcus mitis on host response to metals in reconstructed human skin and oral mucosa

2020 ◽  
Vol 83 (5) ◽  
pp. 347-360
Author(s):  
Lin Shang ◽  
Dongmei Deng ◽  
Sanne Roffel ◽  
Susan Gibbs
Keyword(s):  
Human Skin ◽  
Oral Mucosa ◽  
Host Response ◽  
Streptococcus Mitis ◽  
Reconstructed Human Skin

scholarly journals Label-free, three-dimensional multiphoton microscopy of the connective tissue in the anterior vaginal wall

2014 ◽  
Vol 26 (5) ◽  
pp. 685-691
Author(s):  
Michal Sikora ◽  
David Scheiner ◽  
Cornelia Betschart ◽  
Daniele Perucchini ◽  
José María Mateos ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Multiphoton Microscopy ◽  
Three Dimensional ◽  
Vaginal Wall ◽  
Anterior Vaginal Wall ◽  
Label Free

Correlative multiphoton microscopy and infrared nanospectroscopy of label-free collagen

2019 ◽  
Author(s):  
Jérémie Mathurin ◽  
Gervaise Mosser ◽  
Alexandre Dazzi ◽  
Ariane Deniset-Besseau ◽  
Marie-Claire Schanne-Klein ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free

scholarly journals Ultraviolet A irradiation induces ultraweak photon emission with characteristic spectral patterns from biomolecules present in human skin

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Katsuhiko Tsuchida ◽  
Masaki Kobayashi
Keyword(s):  
Oxidative Stress ◽  
Human Skin ◽  
Skin Diseases ◽  
Photon Emission ◽  
Skin Tissue ◽  
Label Free ◽  
Ultraviolet A ◽  
Living Body ◽  
Non Invasive ◽  
Spectral Patterns

AbstractOxidative stress is associated with photoaging of the skin as well as with skin cancer, and is therefore, critical to monitor. Ultraweak photon emission (UPE) is extremely weak light generated during the oxidative process in the living body and has been used as a non-invasive and label-free marker for the evaluation of oxidative stress. However, the mechanism of UPE generation is not clear. Therefore, we aimed to elucidate the molecular mechanism underlying UPE generation by analyzing the spectra of UPE generated from biomolecules in the skin during ultraviolet A (UVA) exposure. The spectra of UVA-induced UPE generated from linoleic acid, linolenic acid, elastin, phospholipids, and 5,6-dihydroxyindole-2-carboxylic acid were measured, and the spectrum of human skin tissue was also obtained. The spectral patterns varied for the different biomolecules and the peaks were distinct from those of the skin tissue. These results suggested that the UPE generated from skin tissue is a collection of light emitted by biomolecules. Moreover, we proposed that UPE is generated through a photosensitization reaction and energy transfer. The identified characteristic spectral patterns of UPE can be useful to elucidate UVA-induced oxidative stress in the skin, with implications for prevention and treatment of photoaging and skin diseases.

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