Identification and Characterization of Protein Posttranslational Modifications by Differential Fluorescent Labeling

2015 ◽  
pp. 243-262
Author(s):  
Eric D. Hamlett ◽  
Cristina Osorio ◽  
Oscar Alzate
Keyword(s):  
Posttranslational Modifications ◽  
Fluorescent Labeling ◽  
Protein Posttranslational Modifications ◽  
Identification And Characterization

scholarly journals N-glycan chitobiose core biosynthesis by Agl24 strengthens the hypothesis of an archaeal origin of the eukaryal N-glycosylation

2021 ◽  
Author(s):  
Benjamin H. Meyer ◽  
Ben A. Wagstaff ◽  
Panagiotis S. Adam ◽  
Sonja-Verena Albers ◽  
Helge C. Dorfmueller
Keyword(s):  
Posttranslational Modifications ◽  
Biochemical Characterization ◽  
Similar Process ◽  
List Type ◽  
Histidine Residue ◽  
Functional Importance ◽  
Domains Of Life ◽  
Distant Relation ◽  
Identification And Characterization

AbstractProtein N-glycosylation is the most common posttranslational modifications found in all three domains of life. The crenarchaeal N-glycosylation begins with the synthesis of a lipid-linked chitobiose core structure, identical to that in eukaryotes. Here, we report the identification of a thermostable archaeal beta-1,4-N-acetylglucosaminyltransferase, named archaeal glycosylation enzyme 24 (Agl24), responsible for the synthesis of the N-glycan chitobiose core. Biochemical characterization confirmed the function as an inverting β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol glycosyltransferase. Substitution of a conserved histidine residue, found also in the eukaryotic and bacterial homologs, demonstrated its functional importance for Agl24. Furthermore, bioinformatics and structural modeling revealed strong similarities between Agl24 and both the eukaryotic Alg14/13 and a distant relation to the bacterial MurG, which catalyze the identical or a similar process, respectively. Our data, complemented by phylogenetic analysis of Alg13 and Alg14, revealed similar sequences in Asgardarchaeota, further supporting the hypothesis that the Alg13/14 homologs in eukaryotes have been acquired during eukaryogenesis.HighlightsFirst identification and characterization of a thermostable β-D-GlcNAc-(1→4)-α-D-GlcNAc-diphosphodolichol glycosyltransferase (GT family 28) in Archaea.A highly conserved histidine, within a GGH motif in Agl24, Alg14, and MurG, is essential for function of Agl24.Agl24-like homologs are broadly distributed among Archaea.The eukaryotic Alg13 and Alg14 are closely related to the Asgard homologs, suggesting their acquisition during eukaryogenesis.

scholarly journals Computational identification of miRNAs and their targets from Crocus sativus L.

2012 ◽  
Vol 64 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Praveen Guleria ◽  
Deepmala Goswami ◽  
Kumar Yadav
Keyword(s):  
Posttranslational Modifications ◽  
Stress Responses ◽  
Mrna Export ◽  
Crocus Sativus ◽  
Est Database ◽  
Computational Approaches ◽  
Export Protein ◽  
Identification And Characterization ◽  
Crocus Sativus L

miRNAs are smaller known entities that perform several crucial regulatory roles in plants. Identification and characterization of miRNAs has been a challenging task which has become simplified with the development of computational approaches. In the present study, three novel miRNAs were predicted from Crocus sativus by computational approaches. A total 6767 ESTs were mined from the EST database available at NCBI. The software MicroPC was utilized which resulted with the prediction of three miRNAs, csa-miR1, csa-miR2 and csa-miR3. Targets were predicted for the respective miRNAs using the miRU2 program. The identified targets have a role in plant growth, senescence, disease resistance and various stress responses. In addition, some targets are involved in mRNA export, protein synthesis and posttranslational modifications.

Identification and characterization of peptidases secreted by quahog parasite unknown (QPX), the protistan parasite of hard clams

2016 ◽  
Vol 122 (1) ◽  
pp. 21-33 ◽  
Author(s):  
E Rubin ◽  
GT Werneburg ◽  
E Pales Espinosa ◽  
DG Thanassi ◽  
B Allam
Keyword(s):  
Quahog Parasite Unknown ◽  
Hard Clams ◽  
Identification And Characterization

Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor

2013 ◽  
Vol 103 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Q Jiang ◽  
L Shi ◽  
C Ke ◽  
W You ◽  
J Zhao
Keyword(s):  
Vibrio Harveyi ◽  
Haliotis Diversicolor ◽  
Identification And Characterization

IDENTIFICATION AND CHARACTERIZATION OF 1,2-BN CYCLOHEXENE USING MICROWAVE SPECTROSCOPY

2016 ◽  
Author(s):  
Stephen Kukolich ◽  
Shih-Yuan Liu ◽  
Jacob Ishibashi ◽  
Adam Daly ◽  
Ming Sun
Keyword(s):  
Microwave Spectroscopy ◽  
Identification And Characterization

Identification and characterization of a CRM1/XPO1-dependent nuclear export signal in the human androgen receptor

2016 ◽  
Author(s):  
Stefanie V Schutz ◽  
Axel Merseburger ◽  
Anca Azoitei ◽  
Marcus V Cronauer
Keyword(s):  
Androgen Receptor ◽  
Nuclear Export ◽  
Nuclear Export Signal ◽  
Export Signal ◽  
Identification And Characterization ◽  
Human Androgen Receptor

Clinical evaluation and genetic analysis of patients affected by premature ovarian insufficiency: Identification and characterization of a new mutation of the BMP-15

2020 ◽  
Author(s):  
Francesca Orsolini ◽  
Eleonora Ferrarini ◽  
Patrizia Agretti ◽  
Marco Giuseppina De ◽  
Cosmo Caterina Di ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Clinical Evaluation ◽  
Premature Ovarian Insufficiency ◽  
Ovarian Insufficiency ◽  
New Mutation ◽  
Identification And Characterization

Identification and characterization of aldose reductase in cultured rat mesangial cells

Diabetes ◽  
1992 ◽  
Vol 41 (9) ◽  
pp. 1165-1171 ◽  
Author(s):  
R. Kikkawa ◽  
K. Umemura ◽  
M. Haneda ◽  
N. Kajiwara ◽  
S. Maeda ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
Mesangial Cells ◽  
Identification And Characterization
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