scholarly journals Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation

2020 ◽  
pp. 183-199 ◽  
Author(s):  
Asako Isogawa ◽  
Robert P. Fuchs ◽  
Shingo Fujii
Keyword(s):  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Dna Triple Helix

5-Substituted 2-AminopyridineC-Nucleosides as Protonated Cytidine Equivalents:  Increasing Efficiency and Selectivity in DNA Triple-Helix Formation

1997 ◽  
Vol 119 (24) ◽  
pp. 5499-5511 ◽  
Author(s):  
Stefan Hildbrand ◽  
Adrian Blaser ◽  
Serge P. Parel ◽  
Christian J. Leumann
Keyword(s):  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Dna Triple Helix

scholarly journals Stability of an RNA•DNA–DNA triple helix depends on base triplet composition and length of the RNA third strand

2019 ◽  
Vol 47 (14) ◽  
pp. 7213-7222 ◽  
Author(s):  
Charlotte N Kunkler ◽  
Jacob P Hulewicz ◽  
Sarah C Hickman ◽  
Matthew C Wang ◽  
Phillip J McCown ◽  
...  
Keyword(s):  
Relative Stability ◽  
Triple Helix ◽  
Multiple Factors ◽  
Dna And Rna ◽  
Helix Formation ◽  
Canonical Base ◽  
Triple Helix Formation ◽  
Triple Helices ◽  
Dna Triple Helix ◽  
The Stability

AbstractRecent studies suggest noncoding RNAs interact with genomic DNA, forming an RNA•DNA–DNA triple helix that regulates gene expression. However, base triplet composition of pyrimidine motif RNA•DNA–DNA triple helices is not well understood beyond the canonical U•A–T and C•G–C base triplets. Using native gel-shift assays, the relative stability of 16 different base triplets at a single position, Z•X–Y (where Z = C, U, A, G and X–Y = A–T, G–C, T–A, C–G), in an RNA•DNA–DNA triple helix was determined. The canonical U•A–T and C•G–C base triplets were the most stable, while three non-canonical base triplets completely disrupted triple-helix formation. We further show that our RNA•DNA–DNA triple helix can tolerate up to two consecutive non-canonical A•G–C base triplets. Additionally, the RNA third strand must be at least 19 nucleotides to form an RNA•DNA–DNA triple helix but increasing the length to 27 nucleotides does not increase stability. The relative stability of 16 different base triplets in DNA•DNA–DNA and RNA•RNA–RNA triple helices was distinctly different from those in RNA•DNA–DNA triple helices, showing that base triplet stability depends on strand composition being DNA and/or RNA. Multiple factors influence the stability of triple helices, emphasizing the importance of experimentally validating formation of computationally predicted triple helices.

scholarly journals Polyamine-linked oligonucleotides for DNA triple helix formation

1993 ◽  
Vol 21 (23) ◽  
pp. 5489-5494 ◽  
Author(s):  
Ching-Hsuan Tung ◽  
Kenneth J. Breslauer ◽  
Stanley Stein
Keyword(s):  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Dna Triple Helix

DNA triple helix formation at (AT)n tracts

1998 ◽  
Vol 26 (3) ◽  
pp. S261-S261
Author(s):  
Darren M. Gowers ◽  
Keith R. Fox
Keyword(s):  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Dna Triple Helix

Oligonucleotide-Directed DNA Triple-Helix Formation: An Approach to Artificial Repressors?

1991 ◽  
Vol 1 (3) ◽  
pp. 277-281 ◽  
Author(s):  
L. JAMES MAHER ◽  
BARBARA WOLD ◽  
PETER B. DERVAN
Keyword(s):  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Dna Triple Helix

scholarly journals Extension of DNA triple helix formation to a neighbouring (AT) n site

FEBS Letters ◽  
1995 ◽  
Vol 360 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Simon P. Chandler ◽  
Keith R. Fox
Keyword(s):  
Triple Helix ◽  
Helix Formation ◽  
Triple Helix Formation ◽  
Dna Triple Helix
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