Recording Channelrhodopsin-Evoked Field Potentials and Startle Responses from Larval Zebrafish

2020 ◽  
pp. 201-220
Author(s):  
Yagmur Idil Ozdemir ◽  
Christina A. Hansen ◽  
Mohamed A. Ramy ◽  
Eileen L. Troconis ◽  
Lauren D. McNeil ◽  
...  
Keyword(s):  
Field Potentials ◽  
Larval Zebrafish ◽  
Evoked Field Potentials

scholarly journals Interleukin-17 is Involved in Neuropathic Pain and Spinal Synapse Plasticity on Mice

2021 ◽  
Author(s):  
Jia-Lu Sun ◽  
Wen-Jing Dai ◽  
Xin-Yuan Shen ◽  
Yu-Qiu Zhang ◽  
Ning Lü
Keyword(s):  
Neuropathic Pain ◽  
Mechanical Allodynia ◽  
Intrathecal Injection ◽  
Neutralizing Antibody ◽  
Interleukin 17 ◽  
Field Potentials ◽  
Spinal Dorsal ◽  
Synapse Plasticity ◽  
Evoked Field Potentials ◽  
C Fiber

Abstract Background: Neuropathic pain seriously affects people’s life, but its mechanism is not clear. Interleukin-17 (IL-17) is a proinflammation cytokine and involved in pain regulation. Our previous study found that IL-17 markedly enhanced the excitatory activity of spinal dorsal neurons in mice spinal slices. The present study attempts to explore if IL-17 contributes to neuropathic pain and spinal synapse plasticity.Methods：A model of spared nerve injury (SNI) was established in C57BL/6J mice and IL-17a mutant mice. The pain-like behaviors was tested, and the expression of IL-17 and its receptor, IL-17RA, was detected. C-fiber evoked field potentials were recorded in vivo. Results: In the spinal dorsal horn, IL-17 predominantly expressed in the superficial spinal astrocytes and IL-17RA expressed mostly in neurons and slightly in astrocytes. The SNI-induced static and dynamic allodynia was significantly prevented by pretreatment of neutralizing IL-17 antibody (intrathecal injection, 2 μg/10 μL) and attenuated in IL-17a mutant mice. Post-treatment of IL-17 neutralizing antibody also partially relieved the established mechanical allodynia. Moreover, spinal long-term potentiation (LTP) of C-fiber evoked field potentials, a substrate for central sensitization, was suppressed by IL-17 neutralizing antibody. Intrathecal injection of IL-17 recombinant protein (0.2 μg/10 μL) mimicked the mechanical allodynia and facilitated the spinal LTP. Conclusions: These data implied that IL-17 in the spinal cord played a crucial role in neuropathic pain and central sensitization.

Hippocampal kindling leads to different changes in paired-pulse depression of local evoked field potentials in CA1 area and in fascia dentata

1992 ◽  
Vol 141 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Willem Kamphuis ◽  
Jan A. Gorter ◽  
Wytse J. Wadman ◽  
Fernando H.Lopes da Silva
Keyword(s):  
Field Potentials ◽  
Paired Pulse ◽  
Fascia Dentata ◽  
Evoked Field Potentials ◽  
Ca1 Area ◽  
Paired Pulse Depression

Modeling of evoked field potentials in hippocampal CA1 area describes their dependence on NMDA and GABA receptors

2001 ◽  
Vol 104 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Armen R Sargsyan ◽  
Costas Papatheodoropoulos ◽  
George K Kostopoulos
Keyword(s):  
Gaba Receptors ◽  
Field Potentials ◽  
Evoked Field Potentials ◽  
Hippocampal Ca1 ◽  
Hippocampal Ca1 Area ◽  
Ca1 Area

An in vitro study of retinotectal transmission in the chick: Role of glutamate and GABA in evoked field potentials

1996 ◽  
Vol 13 (4) ◽  
pp. 747-758 ◽  
Author(s):  
J. C. Dye ◽  
H. J. Karten
Keyword(s):  
Optic Nerve ◽  
Field Potential ◽  
In Vitro Study ◽  
Bipolar Electrode ◽  
Field Potentials ◽  
Negative Wave ◽  
Evoked Field Potentials ◽  
Thin Slices ◽  
Retinal Afferents

AbstractWe have developed two brain slice preparations for studying tectofugal visual pathways in the chick: conventional, 400-μm slices (“thin slices”), and “thick slices” which encompass the rostral pole of the optic tectum and the contralateral optic nerve. Stimulation was delivered with a bipolar electrode positioned in stratum opticum in thin slices and in the contralateral optic nerve in thick slices. While the latter preparation provided a means of exclusively and unambiguously activating retinal afferents, several lines of evidence also indicated that the evoked field potentials in thin slices were chiefly consequent to retinal afferent excitation: (1) the similarity of evoked field potentials in thin slices to those in thick slice preparations; (2) their precise localization in retinorecipient layers as shown by prelabeling from retina with FITC-coupled cholera toxin; (3) transmission delays appropriate for retinal afferents as established with the thick slice preparation; (4) patterns of labeled afferents resulting from applications of Dil crystals to slices fixed after recording; and (5) the similarity in transmitter pharmacology between thin and thick slice preparations. Pharmacological manipulations carried out with bath-applied antagonists indicated that glutamate is the principal retinotectal transmitter. The broadly active glutamate receptor blocker, kynurenic acid, reversibly eliminated the postsynaptic component of the field potential as confirmed with 0 Ca2+ saline. A complete block was also effected by the non-NMDA antagonists CNQX and DNQX. The specific NMDA antagonist, APS, caused a smaller and variable reduction in response amplitude. The GABA antagonist, bicuculline, caused a prolongation of the monosynaptic field epsp in retinorecipient layers and an enhancement of the long-latency, negative wave in cellular layers below, supporting a late, excitation-limiting role for this inhibitory transmitter.

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