Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins

2021 ◽  
pp. 223-232
Author(s):  
Marta Alonso-Gardón ◽  
Raúl Estévez
Keyword(s):  
G Protein ◽  
Tobacco Etch Virus ◽  
Interacting Proteins ◽  
G Protein Coupled Receptor ◽  
Receptor Interacting Proteins ◽  
G Protein Coupled

scholarly journals GRK Inhibition Potentiates Glucagon-Like Peptide-1 Action

2021 ◽  
Vol 12 ◽  
Author(s):  
Seunghun P. Lee ◽  
Jenson Qi ◽  
Guozhang Xu ◽  
Matthew M. Rankin ◽  
James Littrell ◽  
...  
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptor ◽  
Dipeptidyl Peptidase 4 ◽  
Receptor Kinases ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
Receptor Interacting Proteins ◽  
G Protein Coupled

The glucagon-like peptide-1 receptor (GLP-1R) is a G-protein-coupled receptor (GPCR) whose activation results in suppression of food intake and improvement of glucose metabolism. Several receptor interacting proteins regulate the signaling of GLP-1R such as G protein-coupled receptor kinases (GRK) and β-arrestins. Here we evaluated the physiological and pharmacological impact of GRK inhibition on GLP-1R activity leveraging small molecule inhibitors of GRK2 and GRK3. We demonstrated that inhibition of GRK: i) inhibited GLP-1-mediated β-arrestin recruitment, ii) enhanced GLP-1-induced insulin secretion in isolated islets and iii) has additive effect with dipeptidyl peptidase 4 in mediating suppression of glucose excursion in mice. These findings highlight the importance of GRK to modulate GLP-1R function in vitro and in vivo. GRK inhibition is a potential therapeutic approach to enhance endogenous and pharmacologically stimulated GLP-1R signaling.

Interactions between G-protein-coupled receptors and periplakin: a selective means to regulate G-protein activation

2004 ◽  
Vol 32 (5) ◽  
pp. 878-880 ◽  
Author(s):  
G. Milligan ◽  
H. Murdoch ◽  
E. Kellett ◽  
J.H. White ◽  
G.-J. Feng
Keyword(s):  
G Protein ◽  
G Protein Coupled Receptors ◽  
Interacting Proteins ◽  
Yeast Two Hybrid ◽  
Protein Activation ◽  
G Protein Activation ◽  
Melanin Concentrating Hormone ◽  
Receptor Interacting Proteins ◽  
G Protein Coupled ◽  
Two Hybrid

A substantial number of G-protein-coupled receptor-interacting proteins have been identified initially by the use of yeast two-hybrid screens. Using the C-terminal tail of both opioid receptors and the melanin concentrating hormone receptor-1 as bait, the actin and intermediate filament-binding protein periplakin was isolated. In each case, the site of interaction is within helix VIII of the receptor and periplakin limits agonist-mediated G-protein activation potentially by competing with G-protein for this region of the receptor.

Studying G protein-coupled receptor activation using split-tobacco etch virus assays

2011 ◽  
Vol 412 (2) ◽  
pp. 141-152 ◽  
Author(s):  
Minou S. Djannatian ◽  
Sabrina Galinski ◽  
Tobias M. Fischer ◽  
Moritz J. Rossner
Keyword(s):  
G Protein ◽  
Receptor Activation ◽  
Tobacco Etch Virus ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Virus Assays
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