Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System

2021 ◽  
pp. 39-46
Author(s):  
Diego R. Coelho ◽  
Pedro Henrique Carneiro
Keyword(s):  
Ns1 Protein ◽  
Expression And Purification ◽  
Baculovirus System

scholarly journals Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system

2021 ◽  
Author(s):  
Naoki Yamamoto ◽  
Jiro Kikuchi ◽  
Yusuke Furukawa ◽  
Naoya Shibayama
Keyword(s):  
Tyrosine Kinase ◽  
Kinase Activity ◽  
Kinase Inhibitors ◽  
Expression System ◽  
Structural Studies ◽  
Expression And Purification ◽  
Tyrosine Kinase Activity ◽  
Fast Screening ◽  
Baculovirus System

We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae infected by gene-modified nucleopolyhedrovirus, and was purified as an active state. We performed an inhibition assay using 17 potential kinase inhibitors, and succeeded in identifying two potent inhibitors for FLT3-ITD. The result has paved the way for screening FLT3-ITD inhibitors in a fast and easy manner, and also for structural studies.

Expression and Purification of Recombinant Proteins Using the Baculovirus System

2018 ◽  
Vol 123 (1) ◽  
pp. e61
Author(s):  
Cheryl Isaac Murphy ◽  
Helen Piwnica-Worms ◽  
Stefan Grünwald ◽  
William G. Romanow ◽  
Nicole Francis ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Expression And Purification ◽  
Baculovirus System

Expression and Purification of Recombinant Human Tryptase in a Baculovirus System

1996 ◽  
Vol 7 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Kentaro Sakai ◽  
Scott D. Long ◽  
Denise A. Dove Pettit ◽  
Guy A. Cabral ◽  
Lawrence B. Schwartz
Keyword(s):  
Expression And Purification ◽  
Baculovirus System

scholarly journals Expression and purification of Suid Herpesvirus-1 glycoprotein E in the baculovirus system and its use to diagnose Aujeszky’s disease in infected pigs

2013 ◽  
Vol 90 (1) ◽  
pp. 1-8 ◽  
Author(s):  
María Soledad Serena ◽  
Christoph Geisler ◽  
Germán Ernesto Metz ◽  
Santiago Gerardo Corva ◽  
Eduardo Carlos Mórtola ◽  
...  
Keyword(s):  
Expression And Purification ◽  
Glycoprotein E ◽  
Aujeszky's Disease ◽  
Aujeszky’S Disease ◽  
Baculovirus System ◽  
Herpesvirus 1

Expression and Purification of a Recombinant Adenovirus Fiber Knob in a Baculovirus System

Intervirology ◽  
2008 ◽  
Vol 51 (3) ◽  
pp. 189-195 ◽  
Author(s):  
Luis E. Farinha-Arcieri ◽  
Bruna M. Porchia ◽  
Cassiano Carromeu ◽  
Fernando M. Simabuco ◽  
Rodrigo E. Tamura ◽  
...  
Keyword(s):  
Recombinant Adenovirus ◽  
Expression And Purification ◽  
Baculovirus System

Expression and Purification of Enzymatically Active Recombinant Granzyme B in a Baculovirus System

1998 ◽  
Vol 243 (2) ◽  
pp. 384-389 ◽  
Author(s):  
Zhinan Xia ◽  
Chih-Min Kam ◽  
Chifu Huang ◽  
James C. Powers ◽  
Robert J. Mandle ◽  
...  
Keyword(s):  
Granzyme B ◽  
Expression And Purification ◽  
Baculovirus System

Expression and purification of recombinant mouse CRISP4 using a baculovirus system

2020 ◽  
Vol 167 ◽  
pp. 105543
Author(s):  
Avinash S. Gaikwad ◽  
Khai Lee Loh ◽  
Anne E. O'Connor ◽  
Hugh H. Reid ◽  
Moira K. O'Bryan
Keyword(s):  
Expression And Purification ◽  
Recombinant Mouse ◽  
Baculovirus System

scholarly journals Expression and purification of active receptor interacting protein 1 kinase using a baculovirus system

2013 ◽  
Vol 89 (2) ◽  
pp. 156-161 ◽  
Author(s):  
Jenny L. Maki ◽  
J. Tres Brazell ◽  
Xin Teng ◽  
Gregory D. Cuny ◽  
Alexei Degterev
Keyword(s):  
Expression And Purification ◽  
Interacting Protein ◽  
Baculovirus System

High level expression and purification of bioactive bovine interleukin-18 using a baculovirus system

2002 ◽  
Vol 87 (1-2) ◽  
pp. 65-72 ◽  
Author(s):  
Tomoshi Nagata ◽  
Satoko Ishikawa ◽  
Eiko Shimokawa ◽  
Makiyo Kamikawa ◽  
Reiko Kikuma ◽  
...  
Keyword(s):  
Interleukin 18 ◽  
Expression And Purification ◽  
High Level Expression ◽  
Baculovirus System ◽  
High Level
Sign in / Sign up

Export Citation Format

Share Document