1H Nmr Studies of Cooperative Oxygenation of Normal Human Adult Hemoglobin

Abstract Details of a technic for analysis of hemoglobin mixtures using acrylamide-gel electrophoresis are presented. The method offers increased speed and reproducibility. Measurements on the unstained gel are made at 525 and at 420 m µ in a procedure affording increased precision of measurements for minor components. The gel is then stained with amidoblack to detect and measure nonhemoglobin components. The major component of normal human adult hemoglobin is separated into two components in this medium. The two components (A1, A3) appear in varying relative amounts depending on the source of the specimen. The normal minor component A2 also appears. After staining the pattern with a protein dye such as amidoblack 10B, additional components appear in the electrophoresis patterns of hemolysates from carefully washed red cells. These components have a constant migration ratio referred to hemoglobin A1 and appear to be nonheme-containing intraerythrocytic proteins.

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Characterization of new amino-terminal blocking groups in the normal human adult hemoglobin Hb A1b

European Journal of Mass Spectrometry ◽

10.1255/ejms.152 ◽

1995 ◽

Vol 1 (1) ◽

pp. 195

Author(s):

Danielle Promé ◽

Jean- Claude Promé ◽

Yves Bloquit ◽

Jean Rosa

Keyword(s):

Amino Terminal ◽

Human Adult ◽

Normal Human ◽

Adult Hemoglobin

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Proton nuclear magnetic resonance studies of hemoglobins M Boston (.alpha.58E7 His .fwdarw. Tyr) and M Milwaukee (.beta.67E11 Val .fwdarw. Glu): spectral assignments of hyperfine-shifted proton resonances and of proximal histidine (E7) NH resonances to the .alpha. and .beta. chains of normal human adult hemoglobin

Biochemistry ◽

10.1021/bi00564a007 ◽

1980 ◽

Vol 19 (23) ◽

pp. 5196-5202 ◽

Cited By ~ 60

Author(s):

Seizo Takahashi ◽

Allison Lin ◽

Chien Ho

Keyword(s):

Nuclear Magnetic Resonance ◽

Magnetic Resonance ◽

Proton Nuclear Magnetic Resonance ◽

Magnetic Resonance Studies ◽

Human Adult ◽

Normal Human ◽

Adult Hemoglobin ◽

Proton Resonances ◽

Nuclear Magnetic

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1H NMR studies of [N-MeAib1, Tyr(Me)4]ANGII and [N-MeAib1, Tyr(Me)4, Ile8]ANGII in dimethylsulfoxide by nuclear Overhauser effect (NOE) enhancement spectroscopy: cis-trans Isomerism of the His-Pro bond

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19901106 ◽

1990 ◽

Vol 55 (4) ◽

pp. 1106-1111 ◽

Cited By ~ 1

Author(s):

John Matsoukas ◽

Paul Cordopatis ◽

Raghav Yamdagni ◽

Graham J. Moore

Keyword(s):

1H Nmr ◽

Nuclear Overhauser Effect ◽

Nmr Studies ◽

Restricted Rotation ◽

Overhauser Effect ◽

Major Isomer ◽

Conformational Properties ◽

Nuclear Overhauser

The conformational properties of the Sarmesin analogues [N-MeAib1, Tyr(Me)4]ANGII and [N-MeAib1, Tyr(Me)4, Ile8]ANGII in hexadeutero-dimethysulfoxide were investigated by Nuclear Overhauser Effect (NOE) Enhancement Studies. Cis-trans isomers (ratio 1 : 6) due to restricted rotation of the His-Pro bond were observed. Interresidue interactions between the His Cα proton and the two Pro Cδ protons revealed that the major isomer was the trans.

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