Determination of fetal lung maturity by the comparison of one-dimensional L/S ratio, enzymatic lecithin and S/A ratio

1991 ◽  
pp. 64-68
Author(s):  
V. Ragosch ◽  
U. Lorenz ◽  
B. Arabin ◽  
S. Jürgens ◽  
H. Weitzel
Keyword(s):  
Fetal Lung ◽  
One Dimensional ◽  
Fetal Lung Maturity ◽  
Lung Maturity

Improved thin-layer chromatographic determination of phospholipids in gastric aspirate from newborns, for assessment of lung maturity.

1988 ◽  
Vol 34 (4) ◽  
pp. 736-738 ◽  
Author(s):  
D Serrano de la Cruz ◽  
E Santillana ◽  
A Mingo ◽  
G Fuenmayor ◽  
A Pantoja ◽  
...  
Keyword(s):  
Thin Layer ◽  
Fetal Lung ◽  
Chromatographic Determination ◽  
Solvent Mixture ◽  
Gastric Aspirate ◽  
One Dimensional ◽  
Acetic Acid Water ◽  
Chromatographic Resolution ◽  
Lung Maturity

Abstract This one-dimensional thin-layer chromatographic method is used for assay of phospholipids in the gastric aspirate of newborns. The solvent mixture (chloroform/hexane/methanol/glacial acetic acid/water, 12/7/4/3/0.3 by vol) completely resolves lecithin, sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol. The method is simple, precise, inexpensive, and rapid (chromatographic development takes less than 25 min) and gives high chromatographic resolution. We used this method to determine the lecithin/sphingomyelin densitometric ratio (L/S ratio) and the phosphatidylglycerol percentage in 200 samples of gastric aspirate and found an L/S ratio of 2.5 to be a satisfactory cutoff value for distinguishing fetal lung maturity and immaturity. We confirmed that the presence of phosphatidylglycerol excluded the possibility of respiratory distress.

Determination of fetal lung maturity by fluorescence polarization of amniotic fluid

1978 ◽  
Vol 130 (7) ◽  
pp. 782-787 ◽  
Author(s):  
Thomas A. Blumenfeld ◽  
Raymond I. Stark ◽  
L. Stanley James ◽  
John D. George ◽  
Inga Dyrenfurth ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Fluorescence Polarization ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity

Determination of fetal lung maturity in rats by fluorescence polarization

1986 ◽  
Vol 35 (8) ◽  
pp. 1259-1261 ◽  
Author(s):  
Pietro Masturzo ◽  
Fernando Ornaghi ◽  
Mario Salmona
Keyword(s):  
Fluorescence Polarization ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity

scholarly journals DETERMINATION OF FETAL LUNG MATURITY BY FLUORESCENT POLARIZATION (FP) OF AMNIOTIC FLUID

1977 ◽  
Vol 11 (4) ◽  
pp. 413-413 ◽  
Author(s):  
John F Vogt ◽  
Steven A Golde ◽  
Luis A Cabal ◽  
Steven Gabbe ◽  
Joan E Hedgman
Keyword(s):  
Amniotic Fluid ◽  
Fetal Lung ◽  
Fetal Lung Maturity ◽  
Lung Maturity
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