Improved thin-layer chromatographic determination of phospholipids in gastric aspirate from newborns, for assessment of lung maturity.

1988 ◽  
Vol 34 (4) ◽  
pp. 736-738 ◽  
Author(s):  
D Serrano de la Cruz ◽  
E Santillana ◽  
A Mingo ◽  
G Fuenmayor ◽  
A Pantoja ◽  
...  
Keyword(s):  
Thin Layer ◽  
Fetal Lung ◽  
Chromatographic Determination ◽  
Solvent Mixture ◽  
Gastric Aspirate ◽  
One Dimensional ◽  
Acetic Acid Water ◽  
Chromatographic Resolution ◽  
Lung Maturity

Abstract This one-dimensional thin-layer chromatographic method is used for assay of phospholipids in the gastric aspirate of newborns. The solvent mixture (chloroform/hexane/methanol/glacial acetic acid/water, 12/7/4/3/0.3 by vol) completely resolves lecithin, sphingomyelin, phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, and phosphatidylglycerol. The method is simple, precise, inexpensive, and rapid (chromatographic development takes less than 25 min) and gives high chromatographic resolution. We used this method to determine the lecithin/sphingomyelin densitometric ratio (L/S ratio) and the phosphatidylglycerol percentage in 200 samples of gastric aspirate and found an L/S ratio of 2.5 to be a satisfactory cutoff value for distinguishing fetal lung maturity and immaturity. We confirmed that the presence of phosphatidylglycerol excluded the possibility of respiratory distress.

Radial "high-performance" thin-layer chromatography used to assess fetal lung maturity

1990 ◽  
Vol 36 (5) ◽  
pp. 728-731 ◽  
Author(s):  
J R Mackenzie ◽  
M Truesdale
Keyword(s):  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Fetal Lung ◽  
Additional Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Lung Maturity

Abstract A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report results of application of these methods to greater than 2000 specimens of amniotic fluid from both diabetic and nondiabetic cases.

Thin Layer Chromatographic Determination of Sterigmatocystin in Cheese

1985 ◽  
Vol 68 (4) ◽  
pp. 643-645
Author(s):  
Octave J Francis ◽  
George M Ware ◽  
Allens Carman ◽  
Shia S Kuan
Keyword(s):  
Thin Layer ◽  
Aluminum Chloride ◽  
Chromatographic Method ◽  
Chromatographic Determination ◽  
One Dimensional ◽  
Sample Extract ◽  
Ether Mixture ◽  
Final Purification

Abstract A one-dimensional thin layer chromatographic method has been developed for determining sterigmatocystin in cheese. Cheese is extracted with acetonitrile—4% KC1 (85 + 15). A simplified liquid-liquid partition cleanup is used, and the sample extract is passed through a cupric carbonate column for final purification. Sterigmatocystin is visualized by spraying the plate with aluminum chloride. The fluorescence of the : spot is enhanced 10-fold by additional plate spraying with a silicone- ' ether mixture, enabling sterigmatocystin detection and quantitation at 2 land5 μg/kg, respectively. Average recoveries were 88.3 and 86.4% at the 10 and 25 μg/kg levels, respectively.

High-performance thin-layer chromatographic determination of rosiglitazone in its dosage form

2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination

Improved thin-layer chromatography of disaturated phosphatidylcholine in amniotic fluid.

1981 ◽  
Vol 27 (2) ◽  
pp. 239-242 ◽  
Author(s):  
M Y Tsai ◽  
M Cain ◽  
M W Josephson
Keyword(s):  
Respiratory Distress ◽  
Thin Layer ◽  
Amniotic Fluid ◽  
Thin Layer Chromatography ◽  
Fetal Lung ◽  
Dipalmitoyl Phosphatidylcholine ◽  
Fetal Lung Maturity ◽  
Coefficients Of Variation ◽  
Layer Chromatography ◽  
Lung Maturity

Abstract We describe an indirect test of fetal lung maturity: the quantitation of disaturated phosphatidylcholine in amniotic fluid. The lipids in samples of amniotic fluid from 172 patients were reacted with osmium tetroxide, and disaturated phosphatidylcholine was then isolated by thin-layer chromatography. Interfering substances were retained by a pre-adsorbent layer. The charred disaturated phosphatidylcholine, quantitated by densitometry, was compared to standard dipalmitoyl phosphatidylcholine. Both within-run and between-run coefficients of variation were about 10%. Blood and meconium do not interfere. Six infants developed respiratory distress when disaturated phosphatidylcholine concentrations of amniotic fluid drawn within 72 h of delivery were less than 5.5 mg/L. A concurrently determined lecithin/sphingomyelin ratio falsely predicted lung maturity in one of these. In seven other samples for which lecithin/sphingomyelin ratios suggested lung immaturity but disaturated phosphatidyl-choline predicted maturity, none of the infants developed respiratory distress. In normal pregnancies, measurement of disaturated phosphatidylcholine in amniotic fluid appears to be a better predictor of fetal lung maturity than is measurement of the lecithin/sphingomyelin ratio. Further studies are needed to determine if this analysis is a better predictor in diabetic pregnancies.

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