In Vitro Synthesis and Analysis of Plant (1→3)-β-d-glucans and Cellulose: A Key Step Towards the Characterization of Glucan Synthases

2007 ◽  
pp. 123-145 ◽  
Author(s):  
Vincent Bulone
Keyword(s):  
In Vitro Synthesis

scholarly journals Synthesis and Characterization of Mercapturic Acid (N-acetyl-L-cysteine)-Aflatoxin B1 Adduct and Its Quantitation in Rat Urine by an Enzyme Immunoassay

2009 ◽  
Vol 92 (2) ◽  
pp. 487-495 ◽  
Author(s):  
Sujatha Nayak ◽  
Penmatsa Tanuja ◽  
Rao Beedu Sashidhar
Keyword(s):  
Enzyme Immunoassay ◽  
Aflatoxin B1 ◽  
Polyclonal Antibodies ◽  
Mercapturic Acid ◽  
Rat Urine ◽  
In Vitro Synthesis ◽  
Layer Chromatography ◽  
Serum Albumen

Abstract A simple and sensitive indirect noncompetitive enzyme immunoassay to quantitate mercapturic acid-aflatoxin B1 (AFB1) adduct in rat urine is reported. A novel procedure was developed for in vitro synthesis of an immunogen, bovine serum albumen-glutathione-aflatoxin B1 (BSA-GSH-AFB1) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. Sulphydryl group's analysis confirmed the conjugation of SH groups to AFB1. Thin-layer chromatography and spectral analysis (absorption, fluorescence, and Fourier transform infrared) of the conjugates further confirmed the formation of the adducts. Polyclonal antibodies specific to mercapturic acid-AFB1 adduct were produced against BSA-GSH-AFB1. The assay was found to be linear in the range of 100 pg100 ng of the analyte (y ab x). A 50 displacement of BSA-GSH-AFB1 antibodies was achieved at an inhibitory concentration (IC50)of 11.9 ng GSH-AFB1 (r2 0.98) and 1.22 ng N-acetyl-L-cysteine (NAC)-AFB1 (r2 0.98). Spiking 5 g/mL of reference standard to the control rat urine showed a recovery of 98 2. The immunoassay was validated in a rodent model exposed to a single oral dose of 1 mg/kg body mass of pure AFB1. The excretion of NAC-AFB1 adduct was quantitated at the end of 24 h. The concentration of the NAC-AFB1 adduct excreted in urine as determined by the immunoassay was found to be in the range of 3.225.97 g/mg creatinine. The present method may find wide application as a biochemical tool in molecular epidemiological and intervention studies with respect to human exposure to dietary aflatoxins.

Metabolism of imipramine in vitro: synthesis and characterization of N-hydroxydesmethylimipramine

Xenobiotica ◽  
1983 ◽  
Vol 13 (7) ◽  
pp. 391-405 ◽  
Author(s):  
A. H. Beckett ◽  
A. J. Hutt ◽  
G. E. Navas
Keyword(s):  
Synthesis And Characterization ◽  
In Vitro Synthesis

In vitro synthesis and characterization of bacteriochlorophyll-f and its absence in bacteriochlorophyll-e producing organisms

2010 ◽  
Vol 107 (2) ◽  
pp. 133-138 ◽  
Author(s):  
Hitoshi Tamiaki ◽  
Jun Komada ◽  
Michio Kunieda ◽  
Kazuhiro Fukai ◽  
Taichi Yoshitomi ◽  
...  
Keyword(s):  
Synthesis And Characterization ◽  
In Vitro Synthesis ◽  
Bacteriochlorophyll E
Sign in / Sign up

Export Citation Format

Share Document