Rice Functional Genomics by Transposon Mutagenesis

2002 ◽  
Vol 06 (24) ◽  
pp. 930-935 ◽  
Author(s):  
Chang-deok Han
Keyword(s):  
Tissue Culture ◽  
Transposable Elements ◽  
Functional Genomics ◽  
Large Scale ◽  
Expression Patterns ◽  
Transposon Mutagenesis ◽  
Transposon Tagging ◽  
Genomic Sequences ◽  
Knock Out ◽  
Genetic Cross

Transposable elements are powerful mutagens. Along with genomic sequences, knock-out phenotypes and expression patterns are important information to elucidate the function of genes. In this review, I propose a strategy to develop tranposant lines on a large scale by combining genetic cross and tissue culture of Ac and Ds lines. Based on the facts that Ds tends to be inactive in F2 or later generation and Ds becomes reactivated via tissue culture, a large scale of transposants can be produced by tissue culture of seeds carrying Ac and inactive Ds. In this review, I describe limitations and considerations in operating transposon tagging systems in rice. Also, I discuss the efficiency of our gene trap system and technical procedures to clone Ds flanking DNA.

Molecular Cloning of the Maize R-nj Allele by Transposon Tagging with Ac

1988 ◽  
pp. 263-282 ◽  
Author(s):  
Stephen L. Dellaporta ◽  
Irwin Greenblatt ◽  
Jerry L. Kermicle ◽  
James B. Hicks ◽  
Susan R. Wessler
Keyword(s):  
Molecular Cloning ◽  
Transposon Tagging

Transposon Tagging Technologies in Maize

1994 ◽  
pp. 385-401 ◽  
Author(s):  
W. E. Brown ◽  
J. L. Bennetzen
Keyword(s):  
Transposon Tagging

scholarly journals Isolation of cyanobacterial mutants exhibiting growth defects under microoxic conditions by transposon tagging mutagenesis of Synechocystis sp. PCC 6803

2017 ◽  
Vol 63 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Kazuki Terauchi ◽  
Riho Sobue ◽  
Yuho Furutani ◽  
Rina Aoki ◽  
Yuichi Fujita
Keyword(s):  
Transposon Tagging ◽  
Growth Defects ◽  
Pcc 6803

Transposon Tagging with Mutator

1994 ◽  
pp. 243-249 ◽  
Author(s):  
Paul S. Chomet
Keyword(s):  
Transposon Tagging

Selection and characterization of transposon tagging mutants ofSynechocystis sp. PCC 6803 sensitive to high-light and oxidative Stresses

2004 ◽  
Vol 47 (4) ◽  
pp. 289-299 ◽  
Author(s):  
Eun Kyeong Song ◽  
Ismayil S. Zulfugarov ◽  
Jin-Hong Kim ◽  
Eun Ha Kim ◽  
Woo Sung Lee ◽  
...  
Keyword(s):  
High Light ◽  
Transposon Tagging ◽  
Oxidative Stresses ◽  
Pcc 6803
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