scholarly journals Isolation of cyanobacterial mutants exhibiting growth defects under microoxic conditions by transposon tagging mutagenesis of Synechocystis sp. PCC 6803

2017 ◽  
Vol 63 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Kazuki Terauchi ◽  
Riho Sobue ◽  
Yuho Furutani ◽  
Rina Aoki ◽  
Yuichi Fujita
Keyword(s):  
Transposon Tagging ◽  
Growth Defects ◽  
Pcc 6803

Selection and characterization of transposon tagging mutants ofSynechocystis sp. PCC 6803 sensitive to high-light and oxidative Stresses

2004 ◽  
Vol 47 (4) ◽  
pp. 289-299 ◽  
Author(s):  
Eun Kyeong Song ◽  
Ismayil S. Zulfugarov ◽  
Jin-Hong Kim ◽  
Eun Ha Kim ◽  
Woo Sung Lee ◽  
...  
Keyword(s):  
High Light ◽  
Transposon Tagging ◽  
Oxidative Stresses ◽  
Pcc 6803

A study of the impact of mazie SBE I on the [alpha]-polyglucan produced in Synechocystis sp. strain PCC 6803

2007 ◽  
Author(s):  
Shayani Deborah Nesaranjani Pieris
Keyword(s):  
The Impact ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Systematic Humanization of the Yeast Cytoskeleton Discerns Functionally Replaceable from Divergent Human Genes

Genetics ◽  
2020 ◽  
Vol 215 (4) ◽  
pp. 1153-1169 ◽  
Author(s):  
Riddhiman K. Garge ◽  
Jon M. Laurent ◽  
Aashiq H. Kachroo ◽  
Edward M. Marcotte
Keyword(s):  
Gene Families ◽  
Growth Defect ◽  
Gene Duplications ◽  
Yeast Gene ◽  
Macromolecular Transport ◽  
Cellular Processes ◽  
Growth Defects ◽  
Yeast Strains ◽  
Human Genes ◽  
Interaction Partners

Many gene families have been expanded by gene duplications along the human lineage, relative to ancestral opisthokonts, but the extent to which the duplicated genes function similarly is understudied. Here, we focused on structural cytoskeletal genes involved in critical cellular processes, including chromosome segregation, macromolecular transport, and cell shape maintenance. To determine functional redundancy and divergence of duplicated human genes, we systematically humanized the yeast actin, myosin, tubulin, and septin genes, testing ∼81% of human cytoskeletal genes across seven gene families for their ability to complement a growth defect induced by inactivation or deletion of the corresponding yeast ortholog. In five of seven families—all but α-tubulin and light myosin, we found at least one human gene capable of complementing loss of the yeast gene. Despite rescuing growth defects, we observed differential abilities of human genes to rescue cell morphology, meiosis, and mating defects. By comparing phenotypes of humanized strains with deletion phenotypes of their interaction partners, we identify instances of human genes in the actin and septin families capable of carrying out essential functions, but failing to fully complement the cytoskeletal roles of their yeast orthologs, thus leading to abnormal cell morphologies. Overall, we show that duplicated human cytoskeletal genes appear to have diverged such that only a few human genes within each family are capable of replacing the essential roles of their yeast orthologs. The resulting yeast strains with humanized cytoskeletal components now provide surrogate platforms to characterize human genes in simplified eukaryotic contexts.

scholarly journals Molecular cloning and sequencing of the psaD gene encoding subunit II of photosystem I from the cyanobacterium, Synechocystis sp. PCC 6803.

1988 ◽  
Vol 263 (33) ◽  
pp. 17658-17662
Author(s):  
P Reilly ◽  
J D Hulmes ◽  
Y C Pan ◽  
N Nelson
Keyword(s):  
Molecular Cloning ◽  
Photosystem I ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Synechocystis Sp ◽  
Pcc 6803

An effective method for elimination of the defects in diamond caused by physical field asymmetry in high-pressure synthesis cavities

CrystEngComm ◽  
2020 ◽  
Vol 22 (47) ◽  
pp. 8266-8273
Author(s):  
Chunxiao Wang ◽  
Hong-an Ma ◽  
Liangchao Chen ◽  
Shuai Fang ◽  
Jian Wang ◽  
...  
Keyword(s):  
High Pressure ◽  
Physical Field ◽  
Diamond Growth ◽  
High Pressure Synthesis ◽  
Growth Defects ◽  
Pressure Synthesis

FEM was used to explain the mechanism of diamond growth defects and methods were proposed to eliminate the growth defects.

scholarly journals Cloning of the psbK gene from Synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K

1991 ◽  
Vol 266 (17) ◽  
pp. 11111-11115
Author(s):  
M. Ikeuchi ◽  
B. Eggers ◽  
G.Z. Shen ◽  
A. Webber ◽  
J.J. Yu ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Cyanobakterien als Biokatalysatoren

BIOspektrum ◽  
2021 ◽  
Vol 27 (2) ◽  
pp. 208-210
Author(s):  
Marc M. Nowaczyk ◽  
Hanna C. Grimm ◽  
Leen Assil-Companioni ◽  
Robert Kourist
Keyword(s):  
Natural Process ◽  
Oxygenic Photosynthesis ◽  
Phototrophic Microorganisms ◽  
Electron Sink ◽  
Pcc 6803

AbstractThe highly optimized natural process of oxygenic photosynthesis leads to the formation of redox equivalents, such as NADPH, that can be used to fuel heterologous biotransformations in phototrophic microorganisms. We investigated the reduction of 2-methylmaleimide by the ene-reductase YqjM in the cyanobacterium Synechocystis sp. PCC 6803 and doubled the productivity of the cells by inactivating flavodiironproteins (FDPs) as competing electron sink under self-shading conditions, reaching 18.3 mmol h−1 L−1.

Sign in / Sign up

Export Citation Format

Share Document