2008 ◽  
Vol 40 (4) ◽  
pp. 380-382 ◽  
Author(s):  
Laura MacConaill ◽  
Matthew Meyerson
Keyword(s):  

BioTechniques ◽  
2001 ◽  
Vol 31 (4) ◽  
pp. 788-793 ◽  
Author(s):  
J. Li ◽  
F. Wang ◽  
V. Kashuba ◽  
C. Wahlestedt ◽  
E.R. Zabarovsky

2002 ◽  
Vol 68 (5) ◽  
pp. 2316-2325 ◽  
Author(s):  
Nathalie Pradel ◽  
Sabine Leroy-Setrin ◽  
Bernard Joly ◽  
Valérie Livrelli

ABSTRACT To identify Shiga toxin-producing Escherichia coli genes associated with severe human disease, a genomic subtraction technique was used with hemolytic-uremic syndrome-associated O91:H21 strain CH014 and O6:H10 bovine strains. The method was adapted to the Shiga toxin-producing E. coli genome: three rounds of subtraction were used to isolate DNA fragments specific to strain CH014. The fragments were characterized by genetic support analysis, sequencing, and hybridization to the genome of a collection of Shiga toxin-producing E. coli strains. A total of 42 fragments were found, 19 of which correspond to previously identified unique DNA sequences in the enterohemorrhagic E. coli EDL933 reference strain, including 7 fragments corresponding to prophage sequences and others encoding candidate virulence factors, such a SepA homolog protein and a fimbrial usher protein. In addition, the subtraction procedure yielded plasmid-related sequences from Shigella flexneri and enteropathogenic and Shiga toxin-producing E. coli virulence plasmids. We found that lateral gene transfer is extensive in strain CH014, and we discuss the role of genomic mobile elements, especially bacteriophages, in the evolution and possible transfer of virulence determinants.


1992 ◽  
Vol 4 (2) ◽  
pp. 119 ◽  
Author(s):  
Tai-ping Sun ◽  
Howard M. Goodman ◽  
Frederick M. Ausubel
Keyword(s):  

Genetics ◽  
1994 ◽  
Vol 138 (2) ◽  
pp. 289-296 ◽  
Author(s):  
S Kang ◽  
F G Chumley ◽  
B Valent

Abstract Using genomic subtraction, we isolated the mating-type genes (Mat1-1 and Mat1-2) of the rice blast fungus, Magnaporthe grisea. Transformation of M. grisea strains of one mating type with a linearized cosmid clone carrying the opposite mating-type gene resulted in many "dual maters," strains that contain both mating-type genes and successfully mate with Mat1-1 and Mat1-2 testers. Dual maters differed in the frequency of production of perithecia in pure culture. Ascospores isolated from these homothallic crosses were either Mat1-1 or Mat1-2, but there were no dual maters. Most conidia from dual maters also had one or the other of the mating-type genes, but not both. Thus, dual maters appear to lose one of the mating-type genes during vegetative growth. The incidence of self-mating in dual maters appears to depend on the co-occurrence of strains with each mating type in vegetative cultures. In rare transformants, the incoming sequences had replaced the resident mating-type gene. Nearly isogenic pairs produced from three M. grisea laboratory strains were mated to investigate their fertility. One transformant with switched mating type appears to have a mutation that impairs the development of asci when its mating partner has a similar genetic background. The M. grisea Mat1-1 and Mat1-2 genes are idiomorphs approximately 2.5 and 3.5 kb in length, respectively.


2011 ◽  
Vol 77 (3) ◽  
pp. 182-193 ◽  
Author(s):  
Ryo Kubota ◽  
Mark A. Schell ◽  
Gabriel D. Peckham ◽  
Joanne Rue ◽  
Anne M. Alvarez ◽  
...  

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