Sensitive Detection of Ralstonia solanacearum Using Serological Methods and Biolog Automated System

2008 ◽  
pp. 45-53
Author(s):  
A. E. Tawfik ◽  
A. M. M. Mahdy ◽  
A. A. O. El Hafez
Keyword(s):  
Ralstonia Solanacearum ◽  
Sensitive Detection ◽  
Automated System

scholarly journals Sensitive detection of Ralstonia solanacearum in soil: a comparison of different detection techniques

2000 ◽  
Vol 49 (4) ◽  
pp. 414-422 ◽  
Author(s):  
P. M. Pradhanang ◽  
J. G. Elphinstone ◽  
R. T. V. Fox
Keyword(s):  
Ralstonia Solanacearum ◽  
Sensitive Detection ◽  
Detection Techniques

Construction of a TRFIC strip for rapid and sensitive detection of Ralstonia solanacearum

Talanta ◽  
2021 ◽  
pp. 123139
Author(s):  
Ziyan Fan ◽  
Liwei Hu ◽  
Yuan Ji ◽  
Shanshan Liu ◽  
Ying Wang ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Sensitive Detection

scholarly journals Detection of Ralstonia solanacearum by Loop-Mediated Isothermal Amplification

2008 ◽  
Vol 98 (9) ◽  
pp. 1045-1051 ◽  
Author(s):  
R. Kubota ◽  
B. G. Vine ◽  
A. M. Alvarez ◽  
D. M. Jenkins
Keyword(s):  
Ralstonia Solanacearum ◽  
Lamp Assay ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Isothermal Method ◽  
Flic Gene ◽  
Magnesium Pyrophosphate ◽  
Gene Coding ◽  
Colony Forming Units ◽  
Amplification Reaction

Ralstonia solanacearum is a pathogenic bacterium that causes wilt in over 200 plant species. Here we report a rapid and sensitive detection of R. solanacearum using an isothermal method for copying DNA known as loop-mediated amplification (LAMP). A set of four primers was designed to replicate the gene coding for the flagellar subunit, fliC, and conditions for detection were optimized to complete in 60 min at 65°C. Magnesium pyrophosphate resulting from the amplification reaction could be detected optically as an increase in the solution turbidity, and the DNA products spread in a reproducible ladder-like banding pattern after electrophoresis in an agarose gel. Replication of the fliC gene was detected only from R. solanacearum. The detection limit of this LAMP assay was between 104 to 106 colony forming units/ml, and the technique may be useful for developing rapid and sensitive detection methods for the R. solanacearum pathogen in soil and water.

scholarly journals Sensitive, Secure Detection of Race 3 Biovar 2 and Native U.S. Strains of Ralstonia solanacearum

Plant Disease ◽  
2016 ◽  
Vol 100 (3) ◽  
pp. 630-639 ◽  
Author(s):  
Tuan Minh Tran ◽  
Jonathan M. Jacobs ◽  
Alejandra Huerta ◽  
Annett Milling ◽  
Jordan Weibel ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Infected Plant ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Nucleic Acid Binding ◽  
Fta Cards ◽  
Latently Infected ◽  
High Concern ◽  
Quarantine Pathogen ◽  
Infected Tomato

Detecting and correctly identifying Ralstonia solanacearum in infected plants is important because the race 3 biovar 2 (R3bv2) subgroup is a high-concern quarantine pathogen, while the related sequevar 7 group is endemic to the southeastern United States. Preventing accidental import of R3bv2 in geranium cuttings demands sensitive detection methods that are suitable for large-volume use both onshore and offshore. However, detection is complicated by frequent asymptomatic latent infections, uneven pathogen distribution within infected plants, pathogen viable-but-not-culturable state, and biosecurity laws that restrict transport of R3bv2 strains for diagnosis. There are many methods to detect R3bv2 strains but their relative utility is unknown, particularly in the realistic context of infected plant hosts. Therefore, we compared the sensitivity, cost, and technical complexity of several assays to detect and distinguish R3bv2 and sequevar 7 strains of R. solanacearum in geranium, tomato, and potato tissue in the laboratory and in naturally infected tomato plants from the field. The sensitivity of polymerase chain reaction (PCR)-based methods in infected geranium tissues was significantly improved by use of Kapa3G Plant, a polymerase with enhanced performance in the presence of plant inhibitors. R3bv2 cells were killed within 60 min of application to Whatman FTA(R) nucleic acid-binding cards, suggesting that samples on FTA cards can be safely transported for diagnosis. Overall, culture enrichment followed by dilution plating was the most sensitive detection method (101 CFU/ml) but it was also most laborious. Conducting PCR from FTA cards was faster, easier, and sensitive enough to detect approximately 104 CFU/ml, levels similar to those found in latently infected geranium plants.

An automated system to assess pilot performance in a Link GAT-1 trainer.

1974 ◽  
Author(s):  
Peter H. Henry ◽  
Roy A. Turner ◽  
Robert B. Matthie
Keyword(s):  
Automated System ◽  
Pilot Performance
Sign in / Sign up

Export Citation Format

Share Document