Unfolding and Refolding of Disulfide Proteins Using the Method Disulfide Scrambling

2011 ◽  
pp. 91-108
Author(s):  
Rowen J. Y. Chang
Keyword(s):  
Disulfide Scrambling

Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms

2018 ◽  
Vol 20 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Tsutomu Arakawa
Keyword(s):  
Ion Exchange ◽  
Mixed Mode ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Charged State ◽  
Partial Structure ◽  
Reverse Phase ◽  
Oligomer Formation ◽  
Structure Rearrangement ◽  
Disulfide Scrambling

Proteins often generate structure isoforms naturally or artificially due to, for example, different glycosylation, disulfide scrambling, partial structure rearrangement, oligomer formation or chemical modification. The isoform formations are normally accompanied by alterations in charged state or hydrophobicity. Thus, isoforms can be fractionated by reverse-phase, hydrophobic interaction or ion exchange chromatography. We have applied mixed-mode chromatography for fractionation of isoforms for several model proteins and observed that cation exchange Capto MMC and anion exchange Capto adhere columns are effective in separating conformational isoforms and self-associated oligomers.

Evaluation of disulfide scrambling during the enzymatic digestion of bevacizumab at various pH values using mass spectrometry

2016 ◽  
Vol 1864 (9) ◽  
pp. 1188-1194 ◽  
Author(s):  
Wang-Chou Sung ◽  
Chiung-Wen Chang ◽  
Sheng-Yu Huang ◽  
Ting-Yu Wei ◽  
Yi-Li Huang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Enzymatic Digestion ◽  
Ph Values ◽  
Disulfide Scrambling

Disulfide scrambling of interleukin-2: HPLC resolution of the three possible isomers

1986 ◽  
Vol 155 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Jeffrey L. Browning ◽  
Robert J. Mattaliano ◽  
E.Pingchang Chow ◽  
Shu-Mei Liang ◽  
Bernard Allet ◽  
...  
Keyword(s):  
Interleukin 2 ◽  
Disulfide Scrambling

Fast and Slow Tracks in Lysozyme Folding Elucidated by the Technique of Disulfide Scrambling

2009 ◽  
Vol 28 (6) ◽  
pp. 300-304 ◽  
Author(s):  
Jui-Yoa Chang ◽  
Bao-Yuan Lu ◽  
Li Li
Keyword(s):  
Disulfide Scrambling
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