Plantlet Regeneration in vitro via Adventitious Buds and Somatic Embryos in Norway Spruce (Picea Abies)

1988 ◽  
pp. 199-215 ◽  
Author(s):  
Sara von Arnold ◽  
Inger Hakman
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Plantlet Regeneration ◽  
Adventitious Buds ◽  
Regeneration In Vitro

scholarly journals Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Jindřich Bříza ◽  
Daniela Pavingerová ◽  
Josef Vlasák ◽  
Hana Niedermeierová
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Bacillus Thuringiensis ◽  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Marker Gene ◽  
Selection Marker ◽  
Embryogenic Tissue ◽  
35S Promoter ◽  
Rt Pcr

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

scholarly journals Somatic embryos of Picea abies behave like isolated zygotic embryos in vitro but with greatly reduced physiological vigour

2003 ◽  
Vol 69 (2) ◽  
pp. 176-185 ◽  
Author(s):  
C.H. Bornman ◽  
O.S.P. Dickens ◽  
C.F. van der Merwe ◽  
J. Coetzee ◽  
A.-M. Botha ◽  
...  
Keyword(s):  
Picea Abies ◽  
Somatic Embryos ◽  
Zygotic Embryos

Maturation, germination, and conversion of norway spruce (Picea abies L.) somatic embryos to plants

1989 ◽  
Vol 25 (6) ◽  
pp. 575-580 ◽  
Author(s):  
Michael R. Becwar ◽  
Thomas L. Noland ◽  
Judith L. Wyckoff
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos

Possible Effect From Shear Stress on Maturation of Somatic Embryos of Norway Spruce (Picea abies)

2011 ◽  
Vol 108 (5) ◽  
pp. 1089-1099 ◽  
Author(s):  
Hong Sun ◽  
Cyrus K. Aidun ◽  
Ulrika Egertsdotter
Keyword(s):  
Shear Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos

Bud induction on isolated needles of Norway spruce (Picea abies L. Karst.) grown in vitro

1979 ◽  
Vol 15 (4) ◽  
pp. 363-372 ◽  
Author(s):  
Sara Von Arnold ◽  
Tage Eriksson
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Bud Induction
Sign in / Sign up

Export Citation Format

Share Document