Maturation, germination, and conversion of norway spruce (Picea abies L.) somatic embryos to plants

1989 ◽  
Vol 25 (6) ◽  
pp. 575-580 ◽  
Author(s):  
Michael R. Becwar ◽  
Thomas L. Noland ◽  
Judith L. Wyckoff
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos

scholarly journals Norway spruce (Picea abies) genetic transformation with modified Cry3A gene of Bacillus thuringiensis.

2013 ◽  
Vol 60 (3) ◽  
Author(s):  
Jindřich Bříza ◽  
Daniela Pavingerová ◽  
Josef Vlasák ◽  
Hana Niedermeierová
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Bacillus Thuringiensis ◽  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Marker Gene ◽  
Selection Marker ◽  
Embryogenic Tissue ◽  
35S Promoter ◽  
Rt Pcr

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by biolistics. The modified Cry3A genes were driven by a 35S promoter and the nptII screenable selection marker gene was used in all vectors. The transgenic ETs were molecularly characterized and converted into mature somatic embryos. Germinating embryos formed plantlets which were finally planted into perlite and their Cry3A gene transcription activities were demonstrated by RT-PCR.

Possible Effect From Shear Stress on Maturation of Somatic Embryos of Norway Spruce (Picea abies)

2011 ◽  
Vol 108 (5) ◽  
pp. 1089-1099 ◽  
Author(s):  
Hong Sun ◽  
Cyrus K. Aidun ◽  
Ulrika Egertsdotter
Keyword(s):  
Shear Stress ◽  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos

Evaluation of parameters to characterise germination-competent mature somatic embryos of Norway spruce (Picea abies)

2021 ◽  
Vol 203 ◽  
pp. 55-59
Author(s):  
Kim-Cuong Le ◽  
Aruna B. Weerasekara ◽  
Sonali S. Ranade ◽  
E-M Ulrika Egertsdotter
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Evaluation Of Parameters

scholarly journals Storage lipid dynamics in somatic embryos of Norway spruce (Picea abies): histochemical and quantitative analyses

2007 ◽  
Vol 27 (11) ◽  
pp. 1533-1540 ◽  
Author(s):  
M. Grigova ◽  
M. Kubes ◽  
N. Drazna ◽  
T. Rezanka ◽  
H. Lipavska
Keyword(s):  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Lipid Dynamics ◽  
Storage Lipid ◽  
Quantitative Analyses

scholarly journals Polyamines during somatic embryo development in Norway spruce (Picea abies [L.])

2009 ◽  
Vol 55 (No. 2) ◽  
pp. 75-80 ◽  
Author(s):  
J. Malá ◽  
M. Cvikrová ◽  
P. Máchová ◽  
O. Martincová
Keyword(s):  
Somatic Embryogenesis ◽  
Picea Abies ◽  
Norway Spruce ◽  
Somatic Embryos ◽  
Developmental Stages ◽  
Embryogenic Tissue ◽  
Growth Media ◽  
Mature Embryos ◽  
Proliferation Medium ◽  
Maturation Medium

Contents of free polyamines (putrescine, spermidine and spermine) were determined in different developmental stages of Norway spruce (<I>Picea abies</I> [L.] Karst.) somatic embryos by means of HPLC. Determinations were performed embryogenic tissue after 4 weeks of the growth on proliferation medium, after 2 and 5 weeks of the culturing on maturation medium, and 2 weeks after desiccation. Maturation of somatic embryos (after 5 weeks) was accompanied by increase of concentrations of putrescine (2.3 times) and spermidine (3.2 times). In comparison with above mentioned polyamines, spermine concentrations were significantly lower (4.3 times). Two weeks after desiccation, the concentrations of putrescine decreased 5.4 times and spermidine 2.2 times in comparison with mature embryos. To improve the efficiency of somatic embryogenesis of less responsive genotypes, the supplementation of growth media by polyamines is discussed.

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