Site-Directed Spin Labeling of Membrane Proteins

1994 ◽  
pp. 224-248 ◽  
Author(s):  
Wayne L. Hubbell ◽  
Christian Altenbach
Keyword(s):  
Membrane Proteins ◽  
Spin Labeling ◽  
Site Directed Spin Labeling

scholarly journals Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 763 ◽  
Author(s):  
Indra D. Sahu ◽  
Gary A. Lorigan
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Proteins ◽  
Dynamic Properties ◽  
Conformational Dynamics ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Hydrophobic Nature ◽  
Biophysical Techniques ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic

Membrane proteins possess a variety of functions essential to the survival of organisms. However, due to their inherent hydrophobic nature, it is extremely difficult to probe the structure and dynamic properties of membrane proteins using traditional biophysical techniques, particularly in their native environments. Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling (SDSL) is a very powerful and rapidly growing biophysical technique to study pertinent structural and dynamic properties of membrane proteins with no size restrictions. In this review, we will briefly discuss the most commonly used EPR techniques and their recent applications for answering structure and conformational dynamics related questions of important membrane protein systems.

scholarly journals Analyzing the Viability of Various Native Membrane Mimics for Membrane Proteins using Site-Directed Spin Labeling EPR

2015 ◽  
Vol 108 (2) ◽  
pp. 90a
Author(s):  
Megan M. Dunagan ◽  
Indra D. Sahu ◽  
Rongfu Zhang ◽  
Andrew Craig ◽  
Robert McMarrick ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Spin Labeling ◽  
Membrane Mimics ◽  
Site Directed Spin Labeling

scholarly journals Site-Directed Spin Labeling EPR for Studying Membrane Proteins

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Indra D. Sahu ◽  
Gary A. Lorigan
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Dynamic Properties ◽  
Biological Systems ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Living Organisms ◽  
Electron Paramagnetic

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy is a rapidly expanding powerful biophysical technique to study the structural and dynamic properties of membrane proteins in a native environment. Membrane proteins are responsible for performing important functions in a wide variety of complicated biological systems that are responsible for the survival of living organisms. In this review, a brief introduction of the most popular SDSL EPR techniques and illustrations of recent applications for studying pertinent structural and dynamic properties on membrane proteins will be discussed.

scholarly journals Motional Restrictions of Membrane Proteins: A Site-Directed Spin Labeling Study

2006 ◽  
Vol 91 (9) ◽  
pp. 3341-3348 ◽  
Author(s):  
David Stopar ◽  
Janez Štrancar ◽  
Ruud B. Spruijt ◽  
Marcus A. Hemminga
Keyword(s):  
Membrane Proteins ◽  
Spin Labeling ◽  
Site Directed Spin Labeling ◽  
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