The effect of culture and membrane potential on Goα expression in neonatal rat cardiac myocytes

1991 ◽  
pp. 63-72
Author(s):  
K. A. Foster ◽  
P. J. McDermott ◽  
J. D. Robishaw
Keyword(s):  
Membrane Potential ◽  
Cardiac Myocytes ◽  
Neonatal Rat ◽  
Neonatal Rat Cardiac Myocytes ◽  
Rat Cardiac Myocytes

Role of α1-adrenoceptor subtypes which mediate positive chronotropy in neonatal rat cardiac myocytes

Life Sciences ◽  
1994 ◽  
Vol 54 (24) ◽  
pp. PL451-PL456 ◽  
Author(s):  
Hisakazu Kimura ◽  
Shin Kawana ◽  
Noriaki Kanaya ◽  
Shoji Sakano ◽  
Atsushi Miyamoto ◽  
...  
Keyword(s):  
Cardiac Myocytes ◽  
Neonatal Rat ◽  
Neonatal Rat Cardiac Myocytes ◽  
Rat Cardiac Myocytes

scholarly journals Uptake of thyroid hormones in neonatal rat cardiac myocytes.

Endocrinology ◽  
1996 ◽  
Vol 137 (10) ◽  
pp. 4235-4242 ◽  
Author(s):  
M E Everts ◽  
F A Verhoeven ◽  
K Bezstarosti ◽  
E P Moerings ◽  
G Hennemann ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Cardiac Myocytes ◽  
Neonatal Rat ◽  
Neonatal Rat Cardiac Myocytes ◽  
Rat Cardiac Myocytes

Desmin gene expression in cardiac myocytes is responsive to contractile activity and stretch

1996 ◽  
Vol 270 (4) ◽  
pp. C1228-C1235 ◽  
Author(s):  
P. A. Watson ◽  
R. Hannan ◽  
L. L. Carl ◽  
K. E. Giger
Keyword(s):  
Gene Expression ◽  
Cardiac Myocytes ◽  
Blot Analysis ◽  
Contractile Activity ◽  
Neonatal Rat ◽  
Ang Ii ◽  
Similar Treatment ◽  
Neonatal Rat Cardiac Myocytes ◽  
Mrna Content ◽  
Rat Cardiac Myocytes

Experiments were performed to assess the ability of mechanical stimuli, experienced by ventricular cardiac myocytes during the progression of hypertrophic and dilated pathology, to increase the expression of desmin in cultured neonatal rat cardiac myocytes. Results indicate that both contractile activity and load due to passive stretch increase desmin content in neonatal rat cardiac myocytes through increased desmin gene transcription. Western blot analysis demonstrated that contraction induced a selective increase in desmin protein content in neonatal rat cardiac myocytes above increases observed in the content of total cellular protein. Northern blot analysis indicated that desmin mRNA content increased in response to contraction as well as to alpha-adrenergic stimulation. Desmin mRNA content also increased in cultured neonatal myocytes in response to stretch. Angiotensin II (ANG II) treatment of contracting neonatal cardiac myocytes further increased desmin mRNA content, whereas similar treatment in arrested neonatal cardiac myocytes further increased desmin mRNA content, whereas similar treatment in arrested neonatal cardiac myocytes failed to increase desmin mRNA. This contraction-dependent responsiveness to ANG II is not a function of increases in the density or relative subtype composition of ANG II receptors. Treatment of contracting neonatal rat cardiac myocytes with actinomycin D prevented increases in desmin mRNA content, suggesting regulation of transcription of the desmin gene by contraction. Nuclear run-on experiments indicate that contraction. Nuclear run-on experiments indicate that contraction increases transcription of the desmin gene in cardiac myocytes. These results are consistent with the modulation of desmin gene expression secondarily to changes in the mechanical environment that occur in cardiac tissue undergoing dilation or hypertrophy.

scholarly journals cAMP activates an ATP-permeable pathway in neonatal rat cardiac myocytes

2000 ◽  
Vol 279 (1) ◽  
pp. C173-C187 ◽  
Author(s):  
Alan S. Lader ◽  
Yong-Fu Xiao ◽  
Catherine R. O'Riordan ◽  
Adriana G. Prat ◽  
G. Robert Jackson ◽  
...  
Keyword(s):  
Cardiac Myocytes ◽  
Single Channel ◽  
Atp Release ◽  
Neonatal Rat ◽  
Luciferase Assay ◽  
Neonatal Rat Cardiac Myocytes ◽  
Single Channel Currents ◽  
Rat Cardiac Myocytes ◽  
Camp Stimulation ◽  
R Domain

The molecular mechanisms associated with intracellular ATP release by the heart are largely unknown. In this study the luciferin-luciferase assay and patch-clamp techniques were used to characterize the pathways responsible for ATP release in neonatal rat cardiac myocytes (NRCM). Spontaneous ATP release by NRCM was significantly increased after cAMP stimulation under physiological conditions. cAMP stimulation also induced an anion-selective electrodiffusional pathway that elicited linear, diphenylamine-2-carboxylate (DPC)-inhibitable Cl− currents in either symmetrical MgCl2 or NaCl. ATP, adenosine 5′- O-(3-thiotriphosphate), and the ATP derivatives ADP and AMP, permeated this pathway; however, GTP did not. The cAMP-induced ATP currents were inhibited by DPC and glibenclamide and by a monoclonal antibody raised against the R domain of the cystic fibrosis transmembrane conductance regulator (CFTR). The channel-like nature of the cAMP-induced ATP-permeable pathway was also determined by assessing protein kinase A-activated single channel Cl− and ATP currents in excised inside-out patches of NRCM. Single channel currents were inhibited by DPC and the anti-CFTR R domain antibody. Thus the data in this report demonstrate the presence of a cAMP-inducible electrodiffusional ATP transport mechanism in NRCM. Based on the pharmacology, patch-clamping data, and luminometry studies, the data are most consistent with the role of a functional CFTR as the anion channel implicated in cAMP-activated ATP transport in NRCM.

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