Membrane Specialization and Cytoskeletal Structures in the Synapse and Axon Revealed by the Quick-Freeze, Deep-Etch Method

1983 ◽  
pp. 113-141 ◽  
Author(s):  
Nobutaka Hirokawa
Keyword(s):  
Membrane Specialization

scholarly journals Astrocyte membrane structure: changes after circulatory arrest.

1981 ◽  
Vol 88 (3) ◽  
pp. 660-663 ◽  
Author(s):  
D M Landis ◽  
T S Reese
Keyword(s):  
Membrane Structure ◽  
Circulatory Arrest ◽  
Freeze Fracture ◽  
Rapid Freezing ◽  
Normal Numbers ◽  
Membrane Specialization ◽  
Structure Changes ◽  
Glial Membrane ◽  
The Brain

Membranes of the astrocytic processes investing small blood vessels and the surface of the brain contain numerous arrays of orthogonally packed particles as revealed by the freeze-fracture technique. The structure of these particle arrays, which we have termed "assemblies," is the same whether tissue is prepared for freeze-fracture by conventional fixation or by quick excision and rapid freezing. However, assemblies are progressively replaced by amorphous clumps and then disappear as the interval between decapitation and rapid freezing increases. Nearly normal numbers of assemblies may be maintained in cerebellar slices in vitro, but there too they disappear at low PO2 or in the presence of dinitrophenol. No other neuronal or glial membrane specialization exhibits a comparable lability.

The ciliary junction: a unique membrane specialization in the ciliate, Glaucoma ferox

1981 ◽  
Vol 77 (3) ◽  
pp. 360-365 ◽  
Author(s):  
Roberto Montesano ◽  
Pierre Didier ◽  
Lelio Orci
Keyword(s):  
Membrane Specialization

scholarly journals Correction: Neuron Specific Rab4 Effector GRASP-1 Coordinates Membrane Specialization and Maturation of Recycling Endosomes

PLoS Biology ◽  
2010 ◽  
Vol 8 (9) ◽  
Author(s):  
Casper C. Hoogenraad ◽  
Ioana Popa ◽  
Kensuke Futai ◽  
Emma Martinez-Sanchez ◽  
Phebe S. Wulf ◽  
...  
Keyword(s):  
Recycling Endosomes ◽  
Membrane Specialization

New Evidence for Membrane Specialization in the Diatom Thalassiosira Antarctica Comber

1980 ◽  
Vol 38 ◽  
pp. 624-625
Author(s):  
Gregory J. Doucette
Keyword(s):  
Electron Microscopy ◽  
Thin Section ◽  
Freeze Fracture ◽  
Marine Diatom ◽  
Supplementary Data ◽  
Ultra Structure ◽  
Membrane Specialization ◽  
Freeze Fracture Electron Microscopy ◽  
New Evidence ◽  
Fracture Electron

The present investigation was undertaken to examine the nonsiliceous ultra-structure of diatoms (BacilIariophyta) by means of freeze fracture electron microscopy. Freeze fracture procedures are complicated by the difficulties encountered in fracturing silica-based components and the removal of these materials subsequent to replica casting. Supplementary data was obtained through conventional thin section methodologies. This report is a prelim¬inary account of observations made on selected nonsiliceous, cellular con¬stituents of Thalassiosira antarctica Comber, a planktonic, marine diatom.

scholarly journals A simple method for freeze-fracture of monolayer cultures.

1975 ◽  
Vol 67 (3) ◽  
pp. 904-911 ◽  
Author(s):  
T R Collins ◽  
J C Bartholomew ◽  
M Calvin
Keyword(s):  
Freeze Fracture ◽  
Silicon Monoxide ◽  
Cellular Interaction ◽  
3T3 Cells ◽  
Simple Method ◽  
Mouse Embryo Fibroblasts ◽  
Membrane Specialization ◽  
Monolayer Cultures ◽  
Growth Properties

A simple method is described for the freeze-fracture in situ of monolayer cultures grown on gold carriers coated with a thin layer of silicon monoxide. Preliminary observations on 3T3 mouse embryo fibroblasts indicate that this technique exposes large areas of cell membrane, making it possible to determine how areas of membrane specialization are related to the cell as a whole and to regions of cellular interaction. 3T3 cells cultured on silicon monoxide show no modification of growth properties compared to cells growing on Falcon plastic, and other cell lines also appear to grow well on this substrate.

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