The Use of Cell Culture Methods for Exploring Teratogenic Susceptibility

1982 ◽  
pp. 59-92 ◽  
Author(s):  
Ruth M. Clayton ◽  
Ahmet Zehir
Keyword(s):  
Cell Culture ◽  
Culture Methods ◽  
Cell Culture Methods

Cell Culture Methods to Evaluate the Biocompatibility of Implant Materials

1992 ◽  
Vol 20 (1) ◽  
pp. 52-60
Author(s):  
Gabriela Ciapetti ◽  
Elisabetta Cenni ◽  
Daniela Cavedagna ◽  
Loredana Pratelli ◽  
Arturo Pizzoferrato
Keyword(s):  
Cell Culture ◽  
Quantitative Data ◽  
Cell Function ◽  
Culture Methods ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Biological Compatibility ◽  
Compatibility Of Materials ◽  
Cell Culture Methods

Cell culture techniques are usually used in the field of biomaterials research and development in order to detect toxic components. Morphological assays are the most widely used methods and give the very first information about the biological compatibility of materials. Cell function assays give more quantitative data, but the comparison of data between different laboratories is difficult. Some of the cell culture methods that are used for biocompatibility studies are described briefly here, and results from our laboratory are reported. Despite some inherent limitations of the cell culture techniques, they are an accurate and reliable method of predicting the biological compatibility of materials to be implanted in vivo.

Abstract P469: Scale-up And Characterization Of Therapeutic Mesenchymal Stromal Cell-derived Extracellular Vesicles

2021 ◽  
Vol 129 (Suppl_1) ◽  
Author(s):  
Selen Uman ◽  
Jason A Burdick
Keyword(s):  
Cell Culture ◽  
Extracellular Vesicles ◽  
Animal Studies ◽  
Scale Up ◽  
Spinner Flask ◽  
Perfusion Bioreactor ◽  
Large Animal ◽  
Culture Methods ◽  
Therapeutic Benefits ◽  
Cell Culture Methods

Introduction: Early studies have shown therapeutic benefits of mesenchymal stromal cells (MSCs) in cardioprotection due to their angiogenic, proliferative, anti-apoptotic and anti-inflammatory properties, which are now attributed to secreted factors such as extracellular vesicles (EVs). While MSC-EVs have shown promise in small animals for cardiovascular therapies, large animal studies are required to evaluate the therapeutic benefit of MSC-EVs for clinical translation. One of the biggest challenges for large animal studies is the need to generate clinically-relevant quality and quantity of EVs without batch-to-batch variations that could compromise efficacy. This study aims to explore three different cell culture methods (traditionally-used tissue culture plates (TCP), 3-D printed bioscaffolds in a perfusion system (P), and microcarriers in dynamic spinner flask conditions (M)) to scale-up the production of MSC-EVs across four different biological donors and rigorously investigate EV yield, size, shape, and content. Methods: MSCs were isolated from the iliac crest of four different Yucatan minipigs using heparinized syringes, and cells were expanded to passage four, at which point they were seeded onto the respective cell culture methods. EVs were collected from conditioned medium (CM) via differential ultracentrifugation. EV size, distribution, yield, and protein concentration were studied using Nanoparticle Tracking Analysis (NTA) and microBCA assays. Results: Both perfusion bioreactor and spinner flask systems enabled sustained maintenance of large numbers of cells. Across biological donors and fabrication methods, modes remained within 50-150 nm and were not statistically different. Microcarrier-based spinner flasks and perfusion bioreactor set-ups both improved EV yield, up to 6 times in efficiency. Ongoing research focuses on examining differences in EV content across biological donors using RNA-sequencing and proteomics.

Cell culture (methods in neurosciences, vol. 2)

1991 ◽  
Vol 14 (10) ◽  
pp. 474-475
Author(s):  
Rae Nishi
Keyword(s):  
Cell Culture ◽  
Culture Methods ◽  
Cell Culture Methods

Accelerated ISAV replication detection by cell culture methods combined with time-monitoring RT-qPCR

2018 ◽  
Vol 42 (2) ◽  
pp. 257-267
Author(s):  
Jean-René Arseneau ◽  
Chantal Gautreau ◽  
Linda Boston ◽  
Michel L. Goguen ◽  
Mark Laflamme
Keyword(s):  
Cell Culture ◽  
Culture Methods ◽  
Cell Culture Methods
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