The Role of ATP in Chromosome Replication Studied in Toluenized Escherichia coli

1974 ◽  
pp. 23-35 ◽  
Author(s):  
Patrick Forterre ◽  
Masamichi Kohiyama
Keyword(s):  
Escherichia Coli ◽  
Chromosome Replication

scholarly journals Analysis of Protein Synthesis Rates after Initiation of Chromosome Replication in Escherichia coli

1999 ◽  
Vol 181 (20) ◽  
pp. 6292-6299 ◽  
Author(s):  
Dorothée Bechtloff ◽  
Björn Grünenfelder ◽  
Thomas Åkerlund ◽  
Kurt Nordström
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chromosome Replication ◽  
Peptide Mass ◽  
Initiation Of Replication ◽  
Mass Mapping ◽  
R1 Replicon

ABSTRACT The aim of this study was to investigate whether the synthesis rates of some proteins change after the initiation of replication inEscherichia coli. An intR1 strain, in which chromosome replication is under the control of an R1 replicon integrated into an inactivated oriC, was used to synchronize chromosome replication, and the rates of protein synthesis were analyzed by two-dimensional polyacrylamide gel electrophoresis of pulse-labeled proteins. Computerized image analysis was used to search for proteins whose expression levels changed at least threefold after initiation of a single round of chromosome replication, which revealed 7 out of about 1,000 detected proteins. The various synthesis rates of three of these proteins turned out to be caused by unbalanced growth and the synthesis of one protein was suppressed in theintR1 strain. The rates of synthesis of the remaining three could be correlated only to the synchronous initiation of replication. These three proteins were analyzed by peptide mass mapping and appeared to be the products of the dps, gapA, andpyrI genes. Thus, the expression of the vast majority of proteins is not influenced by the state of chromosome replication, and a possible role of the replication-associated expression changes of the three identified proteins in the cell cycle is not clear.

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