Gonadotropin Action in Isolated Ovarian Luteal Cells: The Intermediate Role of Adenosine 3′, 5′ Cyclic Monophosphate in Hormonal Stimulation of Progesterone Synthesis

The role of adenosine 3',5'-cyclic monophosphate (cAMP) and protein phosphorylation during beta-adrenergic receptor stimulation of bovine tracheal gland serous cells was investigated in vitro. Isoproterenol, a beta-adrenergic agonist, increased the secretion of 35S-labeled molecules. Intracellular cAMP levels were increased within 1 min after stimulation of bovine tracheal gland serous cells with isoproterenol. The dose-response relationship for isoproterenol-stimulated generation of cAMP correlated with the dose-response relationship for isoproterenol-stimulated secretion of 35S-labeled molecules. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine potentiated both isoproterenol-evoked secretion of 35S-labeled molecules and the production of intracellular cAMP, and the beta-adrenergic receptor antagonist propranolol completely blocked both effects. The secretory response of the cells to isoproterenol could be mimicked by the cAMP analogues 8-bromoadenosine 3',5'-cyclic monophosphate and dibutyryl adenosine 3',5'-cyclic monophosphate. Activity of cAMP-dependent kinase was measured in soluble and particulate cell extracts. cAMP effected the state of phosphorylation of proteins associated with the soluble but not the particulate fraction. These studies are consistent with the hypothesis that beta-adrenergic stimulation of secretion from bovine tracheal gland serous cells occurs via a cAMP-mediated pathway and that one of the molecular events in this pathway is cAMP-dependent protein phosphorylation.

Download Full-text

Regulation of total mitochondrial Ca2+ in perfused liver is independent of the permeability transition pore

AJP Cell Physiology ◽

10.1152/ajpcell.1999.276.6.c1297 ◽

1999 ◽

Vol 276 (6) ◽

pp. C1297-C1302 ◽

Cited By ~ 19

Author(s):

Ove Eriksson ◽

Piero Pollesello ◽

Erika Geimonen

Keyword(s):

Permeability Transition Pore ◽

Permeability Transition ◽

Mitochondrial Content ◽

Hormonal Stimulation ◽

Intact Cells ◽

Mitochondrial Energy Metabolism ◽

Isolated Mitochondria ◽

Rat Livers ◽

Stimulation Of

Triggering of the permeability transition pore (PTP) in isolated mitochondria causes release of matrix Ca2+, ions, and metabolites, and it has been proposed that the PTP mediates mitochondrial Ca2+ release in intact cells. To study the role of the PTP in mitochondrial energy metabolism, the mitochondrial content of Ca2+, Mg2+, ATP, and ADP was determined in hormonally stimulated rat livers perfused with cyclosporin A (CsA). Stimulation of livers perfused in the absence of CsA with glucagon and phenylephrine induced an extensive uptake of Ca2+, Mg2+, and ATP plus ADP by the mitochondria, followed by a release on omission of hormones. In the presence of CsA, the PTP was fully inhibited, but neither the hormone-induced uptake of Ca2+, ATP, or ADP by mitochondria nor their release after washout of hormones was significantly changed. We conclude that the regulation of sustained changes in mitochondrial Ca2+content induced by hormonal stimulation is independent of the PTP.

Download Full-text

Review of data on the role of cortisol at the final stages of spawning migration and the reproductive cycle in sturgeons (Acipenseridae)

Proceedings of the Zoological Institute RAS ◽

10.31610/trudyzin/2019.323.4.412 ◽

2019 ◽

Vol 323 (4) ◽

pp. 412-428

Author(s):

L.V. Bayunova

Keyword(s):

Reproductive Cycle ◽

Coelomic Fluid ◽

Enzyme Linked Immunosorbent Assay ◽

Hormonal Stimulation ◽

Acipenser Gueldenstaedtii ◽

Anadromous Migration ◽

Interrenal Gland ◽

Acipenser Stellatus ◽

Stimulation Of

This review presents data on the role of cortisol (F) at the final stages of the reproductive cycle in migratory sturgeons (Acipenseridae) of the Volga-Caspian region: Russian sturgeon (Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833), stellate sturgeon (Acipenser stellatus Pallas, 1771) and beluga (Huso huso (Linnaeus, 1758) obtained using a special variant of enzyme-linked immunosorbent assay (ELISA), which allowed us to compare the indicators of different years. The dynamics of F level in blood serum (BS) and the state of the interrenal gland (IG) in sturgeons were studied during feeding in the sea and at the beginning of the anadromous migration, as well as at the reproduction in the conditions of hatcheries. Higher levels of F in BS and high functional activity of IG are characteristic of sturgeon migrants when entering the river compared with data for fish during feeding in the sea. Reservation of sturgeon breeders at the hatchery led to decrease of the F serum level; and hormonal stimulation of maturation caused an increase in this indicator. Data on the F content in oocytes and abdominal (coelomic) fluid during maturation of female sturgeon are also presented. The F “profiles” were evaluated in sturgeon breeders under stress impacts that are unavoidable at the conditions of hatchery enterprises, as well as under experimentally simulated stress impacts. Peculiarities of the interaction of the hypothalamic-pituitary-gonadal and hypothalamic-pituitary-interrenal axes in sturgeons during reservation at hatcheries and during hormonal stimulation of fish maturation are discussed. Particular attention is paid to the compilation of data contained in the works performed in the late 90s – early 2000s under the guidance of Dr. Sc., prof. I.A. Barannikova (1926–2017), to whose memory the review is dedicated.

Download Full-text

Hormonal stimulation of mitochondrial glutaminase. Effects of vasopressin, angiotensin II, adrenaline and glucagon

Biochemical Journal ◽

10.1042/bj2100957 ◽

1983 ◽

Vol 210 (3) ◽

pp. 957-960 ◽

Cited By ~ 33

Author(s):

S Corvera ◽

J A García-Sáinz

Keyword(s):

Angiotensin Ii ◽

Hormonal Stimulation ◽

Hormonal Modulation ◽

Glutaminase Activity ◽

Stimulation Of

Adrenaline (through alpha 1-adrenoceptors), vasopressin and angiotensin II stimulate mitochondrial glutaminase activity. This stimulation probably contributes to the ureogenic effect of these hormones. The activity of the enzyme is sensitive to Ca2+ depletion. A role of Ca2+ in hormonal modulation of glutaminase activity is suggested.

Download Full-text