Stimulation of oxytocin and progesterone production by bovine granulosa and luteal cells in vitro

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S103-S104 ◽  
Author(s):  
D. SCHAMS ◽  
R. KOLL
Keyword(s):  
Luteal Cells ◽  
Progesterone Production ◽  
Stimulation Of

scholarly journals Serotonin-induced stimulation of progesterone production by cow luteal cells in vitro

Reproduction ◽  
1986 ◽  
Vol 76 (1) ◽  
pp. 231-238 ◽  
Author(s):  
P. J. Battista ◽  
W. A. Condon
Keyword(s):  
Luteal Cells ◽  
Progesterone Production ◽  
Stimulation Of

Contrasting effects of prolactin on luteal and follicular steroidogenesis

1985 ◽  
Vol 104 (2) ◽  
pp. 241-250 ◽  
Author(s):  
B. Kalison ◽  
M. L. Warshaw ◽  
G. Gibori
Keyword(s):  
Luteal Cell ◽  
Corpora Lutea ◽  
Aromatase Activity ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Progesterone Secretion ◽  
Hypophysectomized Rats ◽  
Stimulation Of

ABSTRACT To determine whether prolactin affects both luteal and follicular production of testosterone and oestradiol, pseudopregnant rats, either intact or hypophysectomized on day 8, were injected daily between days 8 and 9 with 1·5 i.u. human chorionic gonadotrophin (hCG), 250 μg prolactin or a combination of both. Control rats were given vehicle. On day 9, blood was obtained from the ovarian vein and corpora lutea and follicles were isolated and incubated in vitro for 2 h. Administration of hCG to intact rats increased ovarian secretion of testosterone and oestradiol dramatically, but did not affect progesterone secretion. Hypophysectomy on day 8 of pseudopregnancy was followed by a drop in ovarian steroid secretion. Prolactin treatment of hypophysectomized rats markedly enhanced progesterone production but had no stimulatory effect on either testosterone or oestradiol. In contrast, hCG dramatically enhanced ovarian secretion of both testosterone and oestradiol without affecting progesterone secretion. Prolactin administered together with hCG antagonized the stimulation of both testosterone and oestradiol secretion by hCG, yet increased progesterone production. When the specific effects of hCG and prolactin administration on follicles and corpora lutea were studied separately, it was found that hCG treatment in vivo greatly stimulated testosterone and oestradiol production by both tissues in vitro. Since hCG only marginally affected aromatase activity in the follicle, had no effect on aromatase activity in luteal cells and did not increase progesterone synthesis, it appears that hCG acts to increase the formation of androgen substrate for oestradiol biosynthesis. Prolactin, administered with or without hCG, inhibited both basal and hCG-stimulated testosterone and oestradiol synthesis by the follicle. In sharp contrast to its inhibitory effect on follicular production of steroids, prolactin appears to be essential for LH stimulation of testosterone and oestradiol by the corpus luteum. In the absence of prolactin, luteal cells gradually ceased to respond to LH and decreased their output of testosterone and oestradiol. Prolactin administration to hypophysectomized rats did not affect luteal cell production of either steroid. However, corpora lutea of rats treated with prolactin responded to the hCG challenge with an increase in testosterone and oestradiol synthesis. In summary, results of this investigation demonstrate that prolactin affects follicular and luteal production of testosterone and oestradiol in opposite ways. It acts on the follicle to inhibit both basal and LH-stimulated production of testosterone and oestradiol, yet it markedly enhances LH stimulation of testosterone and oestradiol synthesis by luteal cells. J. Endocr. (1985) 104, 241–250

Tokishakuyakusan Stimulates Progesterone and Estradiol-17 β Production by Rat Granulosa Cells and Progesterone, Testosterone and Estradiol-17 β by the Residual Portion of the Follicle in Vitro

1991 ◽  
Vol 19 (02) ◽  
pp. 155-161 ◽  
Author(s):  
Satoshi Usuki
Keyword(s):  
Granulosa Cells ◽  
Cell Level ◽  
Testosterone Production ◽  
Progesterone Production ◽  
Preovulatory Follicles ◽  
Immature Rats ◽  
Serum Gonadotropin ◽  
Tissue Compartments ◽  
Stimulation Of

To examine the possible effects of Tokishakuyakusan (TS) on steroidogenesis by preovulatory follicles at the cell level, the expressed granulosa cells and remaining portion of follicles from pregnant mare's serum gonadotropin (PMS)-treated immature rats were incubated in vitro with increasing concentrations of TS for 3 h. TS significantly stimulated progesterone and estradiol-17 b production, with a predominant stimulation of progesterone, by the expressed granulosa cells, while testosterone production was not stimulated. In the remaining portion of the follicle, TS also significantly stimulated progesterone, testosterone and estradiol-17 b production. Similar to the effect produced by granulosa cells, the stimulatory effect of TS was stronger on progesterone than on testosterone and estradiol-17 b production. These results suggest that TS has a potent, direct stimulatory effect on steroidogenesis, especially progesterone production, by constituent tissue compartments of rat preovulatory follicles in vitro.

In. vitro evaluation of corpus luteum function of cycling and pregnant rhesus monkeys: Progesterone production by dispersed luteal cells

Steroids ◽  
1976 ◽  
Vol 27 (4) ◽  
pp. 543-551 ◽  
Author(s):  
Richard L. Stouffer ◽  
Wilbert E. Nixon ◽  
Bela J. Gulyas ◽  
David.K. Johnson ◽  
Gary D. Hodgen
Keyword(s):  
Corpus Luteum ◽  
Rhesus Monkeys ◽  
In Vitro Evaluation ◽  
Luteal Cells ◽  
Progesterone Production

scholarly journals Oxytocin and vasopressin have no effect on progesterone production and cyclic AMP accumulation by rat luteal cells in vitro

Reproduction ◽  
1984 ◽  
Vol 72 (1) ◽  
pp. 137-141 ◽  
Author(s):  
A. K. Mukhopadhyay ◽  
A. Kumar ◽  
R. Tams ◽  
H. G. Bohnet ◽  
F. A. Leidenberger
Keyword(s):  
Cyclic Amp ◽  
Luteal Cells ◽  
Progesterone Production ◽  
Cyclic Amp Accumulation

Effects of prolactin on steroid production by human luteal cells in vitro

1983 ◽  
Vol 96 (3) ◽  
pp. 499-503 ◽  
Author(s):  
G. J. S. Tan ◽  
J. S. G. Biggs
Keyword(s):  
Menstrual Cycle ◽  
Ovarian Function ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Short Term ◽  
Steroid Production ◽  
Luteal Cells ◽  
Progesterone Production

The effects of prolactin on steroidogenesis were studied in dispersed luteal cells prepared from human corpora lutea of the menstrual cycle. Prolactin, at concentrations of 0·1–1000 ng/ml, had no effect on progesterone production by luteal cells during short-term incubation (3 h). However, in two out of five corpora lutea, higher concentrations of prolactin (100 and 1000 ng/ml) significantly reduced the oestradiol-17β production induced by human chorionic gonadotrophin (hCG; 10 i.u./ml); lower doses of prolactin had little effect. In the remaining corpora lutea, prolactin failed to affect either basal or hCG-induced production of oestradiol-17β. These results are discussed in relation to the mechanism by which prolactin influences human ovarian function.

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