Methodologies to Study Channel-Mediated Ion Fluxes in Membrane Vesicles

1986 ◽  
pp. 127-140 ◽  
Author(s):  
Ana Maria Garcia
Keyword(s):  
Membrane Vesicles ◽  
Ion Fluxes

[17] Measurement of ion fluxes in membrane vesicles using rapid-reaction methods

1989 ◽  
pp. 301-313
Author(s):  
Herbert S. Chase ◽  
Mark D. Gelernt ◽  
Marc C. Debell
Keyword(s):  
Membrane Vesicles ◽  
Ion Fluxes ◽  
Rapid Reaction

Acetylcholine receptor-controlled ion fluxes in membrane vesicles investigated by fast reaction techniques

Nature ◽  
1979 ◽  
Vol 282 (5736) ◽  
pp. 329-331 ◽  
Author(s):  
George P. Hess ◽  
Derek J. Cash ◽  
Hitoshi Aoshima
Keyword(s):  
Acetylcholine Receptor ◽  
Membrane Vesicles ◽  
Ion Fluxes ◽  
Fast Reaction

Nuclear Envelope Dynamics During Mitosis

1988 ◽  
Vol 46 ◽  
pp. 224-225
Author(s):  
Brian Burke
Keyword(s):  
Nuclear Envelope ◽  
Membrane Vesicles ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Animal Cells ◽  
Interphase Chromosome ◽  
Lamin B ◽  
Chromosome Architecture ◽  
Complex Membrane ◽  
Pore Complexes

The nuclear envelope is a complex membrane structure that forms the boundary of the nuclear compartment in eukaryotes. It regulates the passage of macromolecules between the two compartments and may be important for organizing interphase chromosome architecture. In interphase animal cells it forms a remarkably stable structure consisting of a double membrane ouerlying a protein meshwork or lamina and penetrated by nuclear pore complexes. The latter form the channels for nucleocytoplasmic exchange of macromolecules, At the onset of mitosis, however, it rapidly disassembles, the membranes fragment to yield small vesicles and the lamina, which is composed of predominantly three polypeptides, lamins R, B and C (MW approx. 74, 68 and 65 kDa respectiuely), breaks down. Lamins B and C are dispersed as monomers throughout the mitotic cytoplasm, while lamin B remains associated with the nuclear membrane vesicles.

Electrophysiological Characterization of Transport Across Outer Membrane Channels from Gram-Negative Bacteria in Their Native Environment

2019 ◽  
Author(s):  
Jiajun Wang ◽  
Rémi Terrasse ◽  
Jayesh Arun Bafna ◽  
Lorraine Benier ◽  
Mathias Winterhalter
Keyword(s):  
Outer Membrane ◽  
Lipid Membrane ◽  
Membrane Vesicles ◽  
Outer Membrane Vesicles ◽  
Multi Drug Resistance ◽  
Gram Negative Bacteria ◽  
Membrane Channels ◽  
Gram Negative ◽  
Electrophysiological Characterization

Multi-drug resistance in Gram-negative bacteria is often associated with low permeability of the outer membrane. To investigate the role of membrane channels in the uptake of antibiotics, we extract, purify and reconstitute them into artificial planar membranes. To avoid this time-consuming procedure, here we show a robust approach using fusion of native outer membrane vesicles (OMV) into planar lipid bilayer which moreover allows also to some extend the characterization of membrane protein channels in their native environment. Two major membrane channels from <i>Escherichia coli</i>, OmpF and OmpC, were overexpressed from the host and the corresponding OMVs were collected. Each OMV fusion revealed surprisingly single or only few channel activities. The asymmetry of the OMV´s translates after fusion into the lipid membrane with the LPS dominantly present at the side of OMV addition. Compared to conventional reconstitution methods, the channels fused from OMVs containing LPS have similar conductance but a much broader distribution. The addition of Enrofloxacin on the LPS side yields somewhat higher association (<i>k<sub>on</sub></i>) and lower dissociation (<i>k<sub>off</sub></i>) rates compared to LPS-free reconstitution. We conclude that using outer membrane vesicles is a fast and easy approach for functional and structural studies of membrane channels in the native membrane.

PLATELETS RELEASE MEMBRANE VESICLES CARRYING THE MATURE microRNA-221 AND ACTIVATED CASPASE-3 DURING THE PLATELET CONCENTRATE STORAGE

Tsitologiya ◽  
2018 ◽  
Vol 60 (7) ◽  
pp. 563-566
Author(s):  
V. V. Kishenko ◽  
◽  
K. A. Kondratov ◽  
V. Yu. Mikhailovskii ◽  
S. V. Sidorkevich ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Membrane Vesicles ◽  
Platelet Concentrate ◽  
Mature Microrna
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