Mechanisms of NAD Action in Regulation of Renal Brush Border Membrane Transport of Phosphate

1986 ◽  
pp. 59-66 ◽  
Author(s):  
Stephen A. Kempson ◽  
Thomas P. Dousa
Keyword(s):  
Membrane Transport ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Renal Brush Border Membrane ◽  
Renal Brush Border

Diuretic effects on renal brush border membrane transport and metabolism

Life Sciences ◽  
1985 ◽  
Vol 37 (18) ◽  
pp. 1675-1681 ◽  
Author(s):  
James Egel ◽  
James Pfanstiel ◽  
Jules B. Puschett
Keyword(s):  
Membrane Transport ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Renal Brush Border Membrane ◽  
Renal Brush Border

Low-affinity transport of pyroglutamyl-histidine in renal brush-border membrane vesicles

1989 ◽  
Vol 257 (5) ◽  
pp. C971-C975 ◽  
Author(s):  
H. A. Skopicki ◽  
K. Fisher ◽  
D. Zikos ◽  
G. Flouret ◽  
D. R. Peterson
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Luminal Membrane ◽  
Renal Brush Border Membrane ◽  
Proximal Tubular ◽  
Renal Brush Border

These studies were performed to determine if a low-affinity carrier is present in the luminal membrane of proximal tubular cells for the transport of the dipeptide, pyroglutamyl-histidine (pGlu-His). We have previously described the existence of a specific, high-affinity, low-capacity [transport constant (Kt) = 9.3 X 10(-8) M, Vmax = 6.1 X 10(-12) mol.mg-1.min-1] carrier for pGlu-His in renal brush-border membrane vesicles. In the present study, we sought to demonstrate that multiple carriers exist for the transport of a single dipeptide by determining whether a low-affinity carrier also exists for the uptake of pGlu-His. Transport of pGlu-His into brush-border membrane vesicles was saturable over the concentration range of 10(-5)-10(-3) M, yielding a Kt of 6.3 X 10(-5) M and a Vmax of 2.2 X 10(-10) mol.mg-1.min-1. Uptake was inhibited by the dipeptides glycyl-proline, glycyl-sarcosine, and carnosine but not by the tripeptide pyroglutamyl-histidyl-prolinamide. We conclude that 1) pGlu-His is transported across the luminal membrane of the proximal tubule by multiple carriers and 2) the lower affinity carrier, unlike the higher affinity carrier, is nonspecific with respect to other dipeptides.

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